Recombinant Human BMP-8a Protein Summary
Product Specifications
Ala264-His402 & Val265-His402
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1073-BP
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 20 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1073-BP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: BMP-8a
BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7 (OP-1) (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs (2). BMPs play roles in regulating growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal, epithelial, hematopoetic, and neuronal cells.
There exist two highly related and closely linked genes, designated BMP-8a and -8b in mice and humans. For humans, the protein products of these two genes share 98% amino acid (aa) sequence identity in their pro- and mature regions. However in the mouse, the two proteins share 89% and 76% aa sequence homology in their pro- and mature regions, respectively (3). Mature human BMP-8a shares 91% and 70% aa sequence identity with mouse BMP-8a and -8b, respectively. Human BMP‑8a is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RTPR) between aa residues 263 and 264 to release a 139 aa carboxy‑terminal domain. Expression patterns of the BMP-8 genes indicate that they regulate aspects of cell proliferation and/or differentiation during spermatogenesis and formation of the placenta (3). BMP-8 is also highly expressed in osteosarcomas (4).
- Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
- Canalis, E. et al. (2003) Endocrine Rev. 24:218.
- Zhao, G-Q. et al. (1996) Mech. Dev. 57:159.
- Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.
Citations for Recombinant Human BMP-8a Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Formative pluripotent stem cells show features of epiblast cells poised for gastrulation
Authors: X Wang, Y Xiang, Y Yu, R Wang, Y Zhang, Q Xu, H Sun, ZA Zhao, X Jiang, X Wang, X Lu, D Qin, Y Quan, J Zhang, N Shyh-Chang, H Wang, N Jing, W Xie, L Li
Cell Research, 2021-02-19;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Genetic dissection of Nodal and Bmp signalling requirements during primordial germ cell development in mouse
Authors: AD Senft, EK Bikoff, EJ Robertson, I Costello
Nat Commun, 2019-03-06;10(1):1089.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming
Authors: AD Senft, I Costello, HW King, AW Mould, EK Bikoff, EJ Robertson
Cell Rep, 2018-08-21;24(8):1977-1985.e7.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
In vitro differentiation of human embryonic stem cells into ovarian follicle-like cells
Authors: D Jung, J Xiong, M Ye, X Qin, L Li, S Cheng, M Luo, J Peng, J Dong, F Tang, W Shen, MM Matzuk, K Kee
Nat Commun, 2017-06-12;8(0):15680.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies.
Authors: Dominguez, Antonia, Chiang, H Rosari, Sukhwani, Meena, Orwig, Kyle E, Reijo Pera, Renee A
Sci Rep, 2014-09-22;4(0):6432.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Brown fat determination and development from muscle precursor cells by novel action of bone morphogenetic protein 6.
Authors: Sharma A, Huard C, Vernochet C, Ziemek D, Knowlton K, Tyminski E, Paradis T, Zhang Y, Jones J, von Schack D, Brown C, Milos P, Coyle A, Tremblay F, Martinez R
PLoS ONE, 2014-03-21;9(3):e92608.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Generation of human induced pluripotent stem cells using epigenetic regulators reveals a germ cell-like identity in partially reprogrammed colonies.
Authors: Goyal, Akshi, Chavez, Shawn L, Reijo Pera, Renee A
PLoS ONE, 2013-12-12;8(12):e82838.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells.
Authors: Geens M, Sermon KD
J. Assist. Reprod. Genet., 2011-02-12;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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Is catalog #1073-BP recombinant human BMP-8a or BMP-8b?
R&D Systems recombinant human BMP-8a was originally sold with the name BMP-8b. When the product was first introduced, its sequence matched the sequence definition for BMP-8b. With the sequencing of the human genome, there has been revision in accessions numbers which has led to the designation of this protein as BMP-8a. Although there has been no change in this product, the name was updated for consistency with the sequence definition. The product description was updated to recombinant human BMP-8a in 2010 so publications prior to this time may refer to this product as BMP-8b.
Reviews for Recombinant Human BMP-8a Protein
Average Rating: 3 (Based on 2 Reviews)
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