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Recombinant Human C1qTNF11/C1qL4 Protein, CF

Catalog #: 9758-TN Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9758-TN-050
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Recombinant Human C1qTNF11/C1qL4 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL4 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Fc Chimera (Catalog # 9338-BA) that produces 50% of the optimal binding response is 2-10 μg/mL.
Source
E. coli-derived human C1qL4 protein
Gly105-Asp238, with an N-terminal Met and 6-His tag
Accession #
Q86Z23
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
16 kDa
SDS-PAGE
13 kDa, reducing conditions

Product Datasheets

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9758-TN

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9758-TN

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: C1qL4

C1qTNF11 (CTRP11), also known as C1qL4, is an approximately 29 kDa member of the C1qTNF family of secreted proteins (1, 2). Mature human C1qTNF11/C1qL4 contains two distinct domains: a collagen-like region and one C1q-like domain, and can form disulfide-linked heteromers with C1qTNF14/C1qL1 (3, 4). The C1qTNF11/C1qL4 gene is conserved in human, chimpanzee, dog, mouse, rat, zebrafish, and frog. Within the C1q-like domain, human C1qTNF11/C1qL4 shares 100% amino acid (aa) sequence identity with that of mouse, monkey and rat. C1qTNF11/C1qL4 is expressed predominantly in testis and adipocytes, and also at a lower level in skeletal muscle and kidney (4), hippocampus, and cerebral cortex (5). Similar to C1qTNF13/C1qL3, C1qTNF10/C1qL2, and C1qTNF14/C1qL1, C1qTNF11/C1qL4 binds to BAI3 in the cerebral cortex and on cerebellar Purkinje cells (5-8). C1qTNF11/C1qL4 binding to BAI3 induces the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (5, 7). Our in house assay shows that C1qTNF11/C1qL4 also binds to BAI-2.  In addition, C1qTNF11/C1qL4 has been reported to promote angiogenesis via activation of the ERK pathway in HUVEC cells mediated by BAI3 (9). Conversely, in adipocytes, C1qTNF11/C1qL4 treatment decreased ERK1/2 signaling and inhibited adipogenesis (4).

References
  1. Ghebrehiwet, B. et al. (2012) Front. Immunol. 3: 52.
  2. Yuzaki, M. (2010) Eur. J. Neurosci. 32:191.
  3. Ressl, S. et al. (2015) Structure 23:688.
  4. Wei, Z. et al. (2013) J. Biol. Chem. 288:10214.
  5. Sigoillot, S.M. et al. (2015) Cell Rep. 10:820.
  6. Lanoue, V. et al. (2013) Mol. Psychiatry 18:943.
  7. Kakegawa, W. et al. (2015) Neuron 85:316.
  8. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
  9. Liu, F. et al. (2017) Mol. Cell. Biochem. 424:57.
Long Name
Complement Component 1, Q Subcomponent-Like 4
Entrez Gene IDs
338761 (Human); 239659 (Mouse); 300220 (Rat)
Alternate Names
C1qL4; C1qTNF11; complement C1q-like protein 4; complement component 1, q subcomponent-like 4; CTRP11; MGC131708

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