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Recombinant Human Cadherin-11 (CAD-11) Fc Chimera, CF

Catalog #: 1790-CA
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1790-CA-050
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Cadherin-11 (CAD-11) Fc Chimera, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of MDA‑MB‑231 human breast cancer cells. When 7.5 x 104 cells/well are added to Recombinant Human Cadherin-11 Fc Chimera coated plates (20 µg/mL with 100 µL/well), ≥50% of cells will adhere after 1 hour at 37 °C.
Source
Mouse myeloma cell line, NS0-derived human Cadherin-11 protein
Human Cadherin-11
Phe-23-Thr617 (pro) &
Gly54-Thr617 (mature)
Accession # AAA35622		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
AAA35622
N-terminal Sequence
Analysis
Phe23 (pro) & Gly54 (mature)
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
93 kDa (pro), 90 kDa (mature)
SDS-PAGE
110-120 kDa, reducing conditions

Product Datasheets

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1790-CA

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1790-CA

Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cadherin-11

Cadherin-11, also known as OB-Cadherin, is a 120 kDa member of the classical Cadherin family of calcium-dependent homophilic adhesion proteins. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Cadherin-11 is expressed in embryonic mesodermal tissues and contributes to the morphogenesis of the nervous and skeletal systems (2-4). It is expressed on osteoblasts in the adult where it promotes the differentiation of both osteoblasts and chondrocytes (5). Cadherin-11 is up‑regulated on breast cancer and prostate cancer cells which preferentially metastasize to bone (6, 7). It facilitates this metastasis via homophilic adhesion to bone marrow stroma and osteoblast-expressed Cadherin-11 (6‑8). In the synovium, Cadherin‑11 supports adhesion between synoviocytes but promotes cell invasion in synovitis and rheumatoid arthritis (9, 10). Its up‑regulation in the vasculature following injury contributes to intimal hyperplasia by inducing smooth muscle cell migration and proliferation (11). In the nervous system, Cadherin-11 interacts with FGF R1 to promote neurite extension from spinal cord explants (12). Mature human Cadherin‑11 consists of a 564 amino acid (aa) extracellular domain (ECD) with five tandem Cadherin repeats, a 23 aa transmembrane segment, and a 156 aa cytoplasmic domain (13, 14). Within the ECD, human Cadherin-11 shares 97% and 98% aa sequence identity with mouse and rat Cadherin‑11, respectively. An 80 kDa portion of the Cadherin‑11 ECD can be shed by proteolytic cleavage, and this fragment competes with the full length molecule for cell adhesion (3, 15). Alternate splicing of human Cadherin-11 generates an 85 kDa isoform with substituted transmembrane and cytoplasmic regions (14, 15).

References
  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Kimura, Y. et al. (1995) Dev. Biol. 169:347.
  3. McCusker, C. et al. (2009) Mol. Biol. Cell 20:78.
  4. Clendenon, S.G. et al. (2009) Dev. Dyn. 238:1909.
  5. Kii, I. et al. (2004) J. Bone Mineral Res. 19:1840.
  6. Tamura, D. et al. (2008) Int. J. Oncol. 33:17.
  7. Chu, K. et al. (2008) Mol. Cancer Res. 6:1259.
  8. Huang, C.-F. et al. (2010) Cancer Res. 70:4580.
  9. Valencia, X. et al. (2004) J. Exp. Med. 200:1673.
  10. Kiener, H.P. et al. (2009) Arthritis Rheum. 60:1305.
  11. Monahan, T.S. et al. (2007) J. Vasc. Surg. 45:581.
  12. Boscher, C. and R.-M. Mege (2008) Cell. Signal. 20:1061.
  13. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
  14. Okazaki, M. et al. (1994) J. Biol. Chem. 269:12092.
  15. Kawaguchi, J. et al. (1999) J. Bone Mineral Res. 14:764.
Entrez Gene IDs
1009 (Human); 12552 (Mouse)
Alternate Names
CAD11OSF-4; cadherin 11, type 2, OB-cadherin (osteoblast); Cadherin11; Cadherin-11; CDH11; CDHOB; OB; OB-Cadherin; Osteoblast cadherin

Citations for Recombinant Human Cadherin-11 (CAD-11) Fc Chimera, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cadherin-11 promotes the metastasis of prostate cancer cells to bone.
    Authors: Chu K, Cheng CJ, Ye X, Lee YC, Zurita AJ, Chen DT, Yu-Lee LY, Zhang S, Yeh ET, Hu MC, Logothetis CJ, Lin SH
    Mol. Cancer Res., 2008-08-01;6(8):1259-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  2. Fibrogenic fibroblasts increase intercellular adhesion strength by reinforcing individual OB-cadherin bonds.
    Authors: Pittet P, Lee K, Kulik AJ, Meister JJ, Hinz B
    J. Cell. Sci., 2008-02-26;121(0):877-86.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Binding Assay

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