Recombinant Human Cadherin-20 (CAD-20) Protein, CF

Recombinant Human Cadherin-20 (CAD-20) Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Cadherin-20

Cadherin-20 is an 82 kDa (predicted) type II classical cadherin from the cadherin superfamily and one of three Cadherin 7-like genes located in a cluster on chromosome 18 (1, 4). Cadherins are calcium-dependent intercellular glycoproteins containing five extracellular cadherin repeats of about 110 amino acids (aa) with typical conserved sequences (1 - 2). The extracellular cadherin domains bind Ca²⁺ in order for correct protein folding and normal functioning to take place (1). Human Cadherin-20 is synthesized as a precursor that contains a 34 aa signal sequence, a 25 aa propeptide, a 560 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 161 aa cytoplasmic region (SwissProt # Q9HBT6). The ECD contains five cadherin domains, two calcium-binding sites, and four potential sites for N-linked glycosylation. Mature human Caderin-20 is 98% and 97% aa identical to mature mouse and rat Cadherin-20, respectively. Cadherin-20 is expressed in placenta, adult brain, and fetal brain (1). In mice, during embryogenesis, Cadherin-20 was found to be synthesized by the forebrain, anterior neural ridge, developing visual system, primitive external granular layer of the cerebellum and a subset of neural crest cells likely to develop into melanoblasts (3). Cadherin-20 is a cell adhesion protein that preferentially interacts with itself in a homophilic manner in connecting cells (1). It thus may contribute to the sorting of heterogeneous cell types (3). In addition, since disturbance of cellular adhesion is a prerequisite for invasion and metastasis of tumor cells, cadherins are considered prime candidates for tumor suppressor genes (1).

Related Research Areas

• Adhesion Molecules
• Adhesion Molecules in Angiogenesis
• Cadherin Superfamily