Recombinant Human Cadherin-6/KCAD Fc Chimera Protein, CF
Recombinant Human Cadherin-6/KCAD Fc Chimera Protein, CF Summary
Product Specifications
Optimal concentration depends on cell type as well as the application or research objectives.
| Human Cadherin-6 (Thr22-Ala615 & Ser54-Ala615) Accession # P55285 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2715-CA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Cadherin-6/KCAD
Cadherin-6, also known as KCAD or K-Cadherin, is a 110-120 kDa type I transmembrane glycoprotein belonging to the classical Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Human Cadherin-6 is synthesized with an 18 amino acid (aa) signal peptide and a 35 aa N-terminal propeptide. The mature cell surface-expressed protein consists of a 562 amino acid (aa) extracellular domain (ECD) that contains five tandem Cadherin repeats, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (3). Within the ECD, human Cadherin-6 shares 96% and 98% aa sequence identity with mouse and rat Cadherin-10, respectively. Alternate splicing of the human Cadherin-6 mRNA generates additional isoforms with deletions in the extracellular region or a substituted and truncated cytoplasmic domain (4). It interacts homotypically as well as heterotypically with Cadherin-9 and more weakly with Cadherins-7, -10, and -14 (5, 6). Cadherin-6 is expressed primarily in the kidney and brain, both during development and in the adult (3, 7-9). It is enriched in renal proximal tubule epithelial cells and their precursors at sites of cell-cell contact as well as on basolateral membranes (8, 10, 11). In the nervous system, Cadherin-6 is neuronally expressed in discrete regions of the neural plate, neural crest cells that give rise to peripheral nerves, and the brain (7, 12). Cadherin-6 is additionally expressed in the developing retina, osteoclasts and their progenitors, ovarian epithelium, and endometrium during the follicular phase of the menstrual cycle (4, 13-15). It is also expressed in carcinomas of the kidney, ovary, and liver (3, 10, 11, 14).
- Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
- Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
- Shimoyama, Y. et al. (1995) Cancer Res. 55:2206.
- Mbalaviele, G. et al. (1998) J. Cell Biol. 141:1467.
- Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
- Shimoyama, Y. et al. (1999) J. Biol. Chem. 274:11987.
- Inoue, T. et al. (1998) Dev. Dyn. 211:338.
- Xiang Y.-Y. et al. (1994) Cancer Res. 54:3034.
- Cho, E.A. et al. (1998) Development 125:803.
- Paul, R. et al. (1997) Cancer Res. 57:2741.
- Shimazui, T. et al. (2000) Eur. Urol. 38:331.
- Inoue, T. et al. (1997) Dev. Biol. 183:183.
- Ruan, G. et al. (2006) Mech. Dev. 123:881.
- Sellar, G.C. et al. (2001) Cancer Res. 61:6977.
- Getsios, S. et al. (1998) Dev. Dyn. 211:238.
Citations for Recombinant Human Cadherin-6/KCAD Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Raludotatug Deruxtecan, a CDH6-Targeting Antibody-Drug Conjugate with a DNA Topoisomerase I Inhibitor DXd, is Efficacious in Human Ovarian and Kidney Cancer Models
Authors: Suzuki, H;Nagase, S;Saito, C;Takatsuka, A;Nagata, M;Honda, K;Kaneda, Y;Nishiya, Y;Honda, T;Ishizaka, T;Nakamura, K;Nakada, T;Abe, Y;Agatsuma, T;
Molecular cancer therapeutics
Species: N/A
Sample Types: Recombinant Protein
Applications: ELISA Capture -
Cadherin 6 has a functional role in platelet aggregation and thrombus formation.
Authors: Dunne E, Spring CM, Reheman A, Jin W, Berndt MC, Newman DK, Newman PJ, Ni H, Kenny D
Arterioscler. Thromb. Vasc. Biol., 2012-04-26;32(7):1724-31.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay
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