Recombinant Human Cadherin-7 (CAD-7) Protein, CF Summary
Product Specifications
The ED50 for this effect is 1.0-4.0 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Met1-Thr607, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6774-CA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Cadherin-7
Cadherin-7 is an approximately 115 kDa type I transmembrane protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Human Cadherin-7 is synthesized with a 27 amino acid (aa) signal peptide and a 20 aa N-terminal propeptide. The mature cell surface-expressed protein consists of a 738 amino acid (aa) extracellular domain (ECD) that contains five Cadherin repeats, a 21 aa transmembrane segment, and a 157 aa cytoplasmic domain (2, 3). Within the ECD, human Cadherin-7 shares 99% and 97% aa sequence identity with mouse and rat Cadherin-7, respectively. Cadherin-7 interacts homotypically and heterotypically with Cadherin-14 and more weakly with Cadherins-6, -9, and -12 (3, 4). Cellular adhesion mediated by Cadherin-7 is more weak than that mediated by E- or N-Cadherin, although it can be strengthed by Fibronectin binding to Integrins on the same cell (5, 6). Cadherin-7 is localized to discrete regions of the developing nervous system. In chick and mouse, it is expressed in the basal plate of the neural tube, migrating cranial motoneurons, and migrating neural crest cells (4, 7, 8), lateral regions of the hindbrain and migrating Purkinje cell precursors (8, 9), striatum, parahippocampal areas, and somatosensory cortex of the forebrain (10 - 12), neural retina and cochlea (13, 14). Cadherin-7 interactions promote motor axon growth and inhibit axonal branching, whereas Cadherin-6B promotes branching during cranial motorneuron development (7). Cadherin-7 performs a similar function during limb bud development during which it participates in the migration and condensation of mesenchymal cells (15). Cadherin-7 is overexpressed on primary melanoma cells where it binds melanoma inhibitory activity (MIA), a secreted melanoma cell protein that promotes tumor progression (16).
- Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
- Kools, P. et al. (2000) Genomics 68:283.
- Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
- Nakagawa, S. et al. (1995) Development 121:1321.
- Chu, Y.-S. et al. (2006) J. Biol. Chem. 281:2901.
- Martinez-Rico, C. et al. (2009) J. Cell Sci. 123:712.
- Barnes, S.H. et al. (2010) Development 137:805.
- Ju, M.J. et al. (2004) Neuroscience 128:785.
- Luo, J. et al. (2004) Mol. Cell. Neurosci. 25:138.
- Redies, C. et al. (2002) Brain Res. Bull. 57:489.
- Kovjanic, D. and C. Redies (2003) J. Comp. Neurol. 456:95.
- Krishna-K, K. et al. (2011) Neuroscience 175:37.
- Faulkner-Jones, B.E. et al. (1999) Mol. Cell. Neurosci. 14:1.
- Luo, J. et al. (2007) Dev. Dyn. 236:2331.
- Kim, D. et al. (2009) J. Cell. Physiol. 221:161.
- Winklmeier, A. et al. (2009) Cancer Sci. 100:261.
Citation for Recombinant Human Cadherin-7 (CAD-7) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Raludotatug Deruxtecan, a CDH6-Targeting Antibody-Drug Conjugate with a DNA Topoisomerase I Inhibitor DXd, is Efficacious in Human Ovarian and Kidney Cancer Models
Authors: Suzuki, H;Nagase, S;Saito, C;Takatsuka, A;Nagata, M;Honda, K;Kaneda, Y;Nishiya, Y;Honda, T;Ishizaka, T;Nakamura, K;Nakada, T;Abe, Y;Agatsuma, T;
Molecular cancer therapeutics
Species: N/A
Sample Types: Recombinant Protein
Applications: ELISA Capture
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human Cadherin-7 (CAD-7) Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Cadherin-7 (CAD-7) Protein, CF and earn rewards!
Have you used Recombinant Human Cadherin-7 (CAD-7) Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
