Recombinant Human Carboxylesterase 1/CES1 Protein, CF
Recombinant Human Carboxylesterase 1/CES1 Protein, CF Summary
Product Specifications
His19-Glu563, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4920-CE
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Assay Procedure
- Assay Buffer: 50 mM Tris, pH 7.5
- Recombinant Human Carboxylesterase 1/CES1 (rhCES1) (Catalog # 4920-CE)
- Substrate: 4-Nitrophenyl acetate (4-NPA) (Sigma, Catalog # N-8130), 100 mM stock in Acetone
- 96-well Clear Plate (Costar, Catalog # 92592)
- Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rhCES1 to 0.8 ng/µL in Assay Buffer.
- Dilute Substrate to 2 mM in deionized water.
- In a plate load 50 μL of rhCES1 to wells and start the reaction by adding 50 µL of 2 mM Substrate. Include a Substrate Blank containing 50 µL Substrate and 50 µL Assay Buffer.
- Read at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 4-Nitrophenol (Sigma, Catalog # 241326).
Per Well:- rhCES1: 0.040 µg
- Substrate: 1 mM
Reconstitution Calculator
Background: Carboxylesterase 1/CES1
Carboxylesterase 1 is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). They have broad substrate specificity ranging from small molecule esters such as phenylester to long chain fatty acid esters and thioesters. They play a major role as determinants of pharmacokinetic behavior for most therapeutic agents containing an ester. By de-esterification, they can activate or inactivate the agents. They also participate in detoxification of drugs such as cocaine and heroin in serum and liver. The resulting de-esterified metabolites are secreted out in urine. They can also detoxify organophosphate and carbamate analogues used in agrochemicals or chemical nerve agents, such as malathion, sarin, tabun, and VX. In addition to the hydrolytic activity, they can perform transesterification, a reaction important for cholesterol homeostasis. Carboxylesterase deficiency may be associated with non-Hodgkin lymphoma or B-cell lymphocytic leukemia. CES-1 shares the serine hydrolase fold observed in other esterases (3). CES-1 possesses an endoplasmic reticulum retention signal (HIEL) at its C-terminus. The purified rhCES-1 lacks this signal, resulting in its secretion.
- Redinbo, M. R. and Potter, P.M. (2005) Drug Discovery Today 10:313.
- Satoh, T. and Hosokawa, M. (2006) Chem. Biol. Interactions 162:195.
- Fleming, C. D. et al. (2007) Biochemistry 46:5603.
Citations for Recombinant Human Carboxylesterase 1/CES1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Functions of human olfactory mucus and age-dependent changes
Authors: T Shirai, D Takase, J Yokoyama, K Nakanishi, C Uehara, N Saito, A Kato-Namba, K Yoshikawa
Scientific Reports, 2023-01-18;13(1):971.
Species: Human
Sample Types: Saliva
Applications: Bioassay -
Discovery and molecular basis of subtype-selective cyclophilin inhibitors
Authors: AA Peterson, AM Rangwala, MK Thakur, PS Ward, C Hung, IR Outhwaite, AI Chan, DL Usanov, VK Mootha, MA Seeliger, DR Liu
Nature Chemical Biology, 2022-09-26;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Systematic identification of biomarker-driven drug combinations to overcome resistance
Authors: MG Rees, L Brenan, M do Carmo, P Duggan, B Bajrami, M Arciprete, A Boghossian, E Vaimberg, SJ Ferrara, TA Lewis, D Rosenberg, T Sangpo, JA Roth, VK Kaushik, F Piccioni, JG Doench, DE Root, CM Johannesse
Nature Chemical Biology, 2022-03-24;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay -
Activation of Tenofovir Alafenamide and Sofosbuvir in the Human Lung and Its Implications in the Development of Nucleoside/Nucleotide Prodrugs for Treating SARS-CoV-2 Pulmonary Infection
Authors: J Li, S Liu, J Shi, HJ Zhu
Pharmaceutics, 2021-10-11;13(10):.
Species: Human
Sample Types: Whole Tissue
Applications: Enzyme Activity -
Hydrolytic Fate of 3/15-Acetyldeoxynivalenol in Humans: Specific Deacetylation by the Small Intestine and Liver Revealed Using in Vitro and ex Vivo Approaches
Authors: el H Ajandouz, S Berdah, V Moutardier, T Bege, DJ Birnbaum, J Perrier, E Di Pasqual, M Maresca
Toxins (Basel), 2016-07-28;8(8):.
Species: Human
Sample Types: Complex Sample Type
Applications: Bioassay -
Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977.
Authors: Murakami E, Tolstykh T, Bao H, Niu C, Steuer HM, Bao D, Chang W, Espiritu C, Bansal S, Lam AM, Otto MJ, Sofia MJ, Furman PA
J. Biol. Chem., 2010-08-26;285(45):34337-47.
Species: Human
Sample Types: Small Molecule
Applications: Enzyme Assay
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