Recombinant Human CART Protein, CF Summary
Product Specifications
Ala37-Leu116
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10974-CO
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl. |
| Reconstitution | Reconstitute at 500 μg/mL in 4 mM HCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Human CART Protein (Catalog # 10974-CO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 8-10 kDa under reducing conditions.
Reconstitution Calculator
Background: CART
Cocaine and amphetamine regulated transcript, also known as CART, is a neuropeptide protein encoded in human by the CARPT gene (1, 2) and is expressed in brain regions associated with reward such as the nucleus accumbens, also expressed in retinal cells, in pituitary glands, adrenal medulla and pancreas (3, 4). CART peptides can function as neurotransmitters and hormones, promote neuronal development, and are involved in regulating many processes, including food intake, maintenance of body weight, reward and addiction, stress response, psychostimulant effects, and endocrine function (5-7). CART production is up-regulated by CREB (8) a protein thought to be involved with the development of drug addiction, thus CART may be an important therapeutic target in the treatment of stimulant abuse (9). More recently, studies show that CART also has a wide variety of functions including bone remodeling, depression, anxiety, learning and memory as well as physiological processes such osmoregulation and blood pressure (10, 11). CART treatment promotes neuronal development and survival in vitro and in vivo (12-14).
- Douglas, J. and S. Daoud (1996) Gene 169:241.
- Kristensen, P. et al. (1998) Nature 303:72.
- Keller, P.A. et al. (2006) Peptides 27:1328.
- Jensen, P.B. et al. (1999) FEBS Lett 447:139.
- Rogge, G. et al. (2008) Nat. Rev. Neurosci. 9:747.
- ChengPeng, Y. et al. (2017) Front. Cell. Neurosci 11:244.
- Lau, J. and H. Herzog (2014) Front. Neurosci. 8:313.
- Rogge, G.A. et al. (2009) Brain Res. 1251:42.
- Fagergren, P. and Y. Hurd (2007) Physiol. Behav. 92:218.
- Ahmadian-Moghadam, H. et al. (2018) Peptides 110:56.
- Zhi, Y.O. and P. Gavan (2020) Brain Res. 1740:146852.
- Wu, B. et al. (2006) Biochem. Biophys. Res. 347:656.
- Jin, J.L. et al. (2014) Nature 5:10224.
- Sha, D.J. et al. (2013) Neuroreport. 24:698.
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