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Recombinant Human CCL14a/HCC-1 (aa 28-93) Protein

Catalog #: 1578-HC
1 Citation Datasheet / COA / SDS
Newer Version Available: 324-HC
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Discontinued Product

1578-HC has been discontinued and is replaced by 324-HC.

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Recombinant Human CCL14a/HCC-1 (aa 28-93) Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED50 for this effect is 0.15-0.75 ng/mL.
Source
E. coli-derived human CCL14a/HCC-1 protein
MHuman CCL14a/HCC-1
(Gly28-Asn93)
Accession # Q16627		WIGDPS
N-terminusC-terminus
Accession #
Q16627
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
7.9 kDa

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1578-HC (with carrier)

Product Datasheet
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1578-HC/CF (carrier free)

Discontinued Product

1578-HC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CCL14a/HCC-1

HCC-1 (Hemofiltrate CC Chemokine-1) was originally isolated from the hemofiltrate of human patients with chronic renal failure (1). It belongs to the CC chemokine superfamily and has been designated CCL14a. HCC-1/CCL14a cDNA encodes a 93 amino acid (aa) residue precursor with a 19 aa signal peptide that is cleaved to form the 74 aa mature secreted proprotein. By alternative splicing, a second longer isoform named HCC-3/CCL14b, which includes sequences from exon 3, also exists (2). HCC-1/CCL14a is expressed constitutively in various normal tissues including spleen, liver, muscle, gut and bone marrow. It circulates at nanomolar concentrations in human plasma. Different post-translationally modified HCC-1/CCL14a, including O-glycosylated and N-terminally truncated variants of HCC-1/CCL14a, have been identified (3, 4). Whereas the 74 aa mature propeptide is a weak CCR1 agonist, the proteolytically processed truncated HCC-1/CCL14a (aa 28 - 93) is a highly potent agonist of CCR1, CCR5 and to a lesser extent, CCR3. HCC-1/CCL14a (aa 28 - 93) promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1. Activation of the HCC-1/CCL14a propeptide to active peptide is mediated by the urokinase type plasminogen activator or plasmin (5).

References
  1. Schulz-Knappe, P. et al. (1996) J. Exp. Med. 183:295.
  2. Forssmanns, U. et al. (2001) J. Leukocyte Biology 70:357.
  3. Richter, R. et al. (2000) Biochemistry 39:10799.
  4. Munch, J. et al. (2002) Antimicrob. Agents Chemother. 46:982.
  5. Vakili, J. et al. (2001) J. Immunol. 167:3406.
Entrez Gene IDs
6358 (Human)
Alternate Names
C-C motif chemokine 14;CC-1;CC-3;CKB1;HCC-1;HCC-1(1-74);HCC-1/HCC-3;HCC-3;MCIF;NCC2;NCC-2;SCYA14;SCYL2;SY14; CCL14; CCL14a; HCC1; HCC-1

Citation for Recombinant Human CCL14a/HCC-1 (aa 28-93) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Possible Roles of CC- and CXC-Chemokines in Regulating Bovine Endometrial Function during Early Pregnancy
    Authors: R Sakumoto, KG Hayashi, S Fujii, H Kanahara, M Hosoe, T Furusawa, K Kizaki
    Int J Mol Sci, 2017-03-31;18(4):.
    Species: Bovine
    Sample Types: Whole Tissue
    Applications: Bioassay

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