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Recombinant Human CCL15/MIP-1 delta/LKN-1 (92 aa) Protein

Catalog #: 363-MG
3 Citations Datasheet / COA / SDS
Newer Version Available: 628-LK
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Discontinued Product

363-MG has been discontinued and is replaced by 628-LK.

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Citations (3)
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Recombinant Human CCL15/MIP-1 delta/LKN-1 (92 aa) Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract THP‑1 human acute monocytic leukemia cells. The ED50 for this effect is 0.2‑0.8 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED50 for this effect is 2-8 ng/mL.
Source
E. coli-derived human CCL15/MIP-1 delta protein
Gln22-Ile113
Accession #
Q16663.2
N-terminal Sequence
Analysis
Gln22
Predicted Molecular Mass
10 kDa

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363-MG (with carrier)

Product Datasheet
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363-MG/CF (carrier free)

Discontinued Product

363-MG

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CCL15/MIP-1 delta

CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-1δ is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

References
  1. Wang, W. et al. (1998) J. Clinical Immunol. 18:214
Entrez Gene IDs
6359 (Human)
Alternate Names
CCL15; chemokine (C-C motif) ligand 15; Chemokine CC-2; HCC-2; HCC-2Lkn-1; Leukotactin 1; Leukotactin-1; LKN1; LKN-1; Macrophage inflammatory protein 5; MIP1 delta; MIP-1 delta; MIP5; MIP-5; MIP-5MIP-1d; Mrp-2b; NCC-3; NCC-3MIP-1D; NCC3MRP-2B; new CC chemokine 3; SCYA15member 15; Small-inducible cytokine A15

Citations for Recombinant Human CCL15/MIP-1 delta/LKN-1 (92 aa) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008-12-01;181(11):8053-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Differential chemokine activation of CC chemokine receptor 1-regulated pathways: ligand selective activation of Galpha 14-coupled pathways.
    Authors: Tian Y, New DC, Yung LY, Allen RA, Slocombe PM, Twomey BM, Lee MM, Wong YH
    Eur. J. Immunol., 2004-03-01;34(3):785-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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