Should I use Recombinant Human CCL2/MCP-1 Protein formulated with carrier BSA (Catalog # 279-MC) or the Recombinant Human CCL2/MCP-1 Protein (Catalog # 279-MC/CF) that is carrier-free (no BSA) for my in vivo experiments?

Name: Recombinant Human CCL2/MCP-1 Protein
Brand: R&D Systems
SKU: 279-MC
Rating: 4.7 (9 reviews)

For in vivo aplications, we would recommend using the carrier-free Recombinant Human CCL2/MCP-1 Protein (Catalog # 279-MC/CF). The injection of a carrier protein such as BSA can cause side effects and sometimes death to the experimental animal. For more information regarding use of the protein for in vivo applications, we would recommend consulting the Citations tab on the product-specific page.

Recombinant Human CCL2/MCP-1 Protein

Catalog #: 279-MC
97 Citations 9 Reviews Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
279-MC-001MG/CF
279-MC-010/CF
279-MC-050/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
279-MC-010
279-MC-050

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Recombinant Human CCL2/MCP-1 Protein Bioactivity

3 Images

All CCL2/JE/MCP-1 Proteins and Enzymes

Product Details

Citations (97)

FAQs

Reviews (9)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CCL2/MCP-1 Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED₅₀ for this effect is 5‑30 ng/mL.

Source

E. coli-derived human CCL2/JE/MCP-1 protein
Gln24-Thr99

Accession #

P13500.1

N-terminal Sequence
Analysis

Gln24

Predicted Molecular Mass

8.7 kDa

SDS-PAGE

10 kDa, reducing conditions

Product Datasheets

279-MC (with carrier)

Product Datasheet

COA

SDS

279-MC/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

279-MC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

279-MC/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human CCL2/ MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A. The ED₅₀ for this effect is 5-30 ng/mL.

SDS-PAGE

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1 μg/lane of Recombinant Human CCL2/MCP-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 10 kDa.

Mass Spectrometry

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MALDI-TOF analysis of Recombinant Human CCL2/MCP-1. The major peak corresponds to the calculated molecular mass, 8679 Da. The minor peak at 8887 is a matrix-associated artifact of the MALDI-TOF.

Reconstitution Calculator

Background: CCL2/JE/MCP-1

CCL2, also called monocyte chemotactic protein‑1 (MCP‑1) or JE, is a member of the C‑C or beta chemokine family that is best known as a chemotactic agent for mononuclear cells (1, 2). Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a 23 aa signal peptide and a 76 aa mature protein (2). Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acid‑modified glutamine, occurs naturally by metalloproteinase cleavage and down‑regulates activity but not receptor binding (3). CCL2 may form multiple bands from 8.7‑13.5 kDa on SDS‑PAGE due to non‑covalent dimerization and variable carbohydrate content (3). Mature human CCL2 shares 78‑79% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 aa and shares only ~56% aa identity within the common region. Human CCL2 can, however, induce a response in murine cells (4). Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source (1, 2). In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor, CCR2 (2). Additionally, it promotes Th2 polarization in CD4⁺ T cells (5). CCL2‑mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma (6, 7).

References

Yoshimura, T. et al. (1989) FEBS Lett. 244:487.
Deshmane, S.L. et al. (2009) J. Interferon Cytokine Res. 29:313.
Proost, P. et al.(1998) J. Immunol. 160:4034.
Ju Lee, H. et al. (2015) J. Immunol. 194:3634.
Luther, S.A. and J.G. Cyster (2001) Nat. Immunol. 2:102.
Daly, C. et al. (2003) Microcirculation 10:247.
Aukrust, P. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1909.

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Alternate Names

C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

Related Research Areas

Citations for Recombinant Human CCL2/MCP-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

97 Citations: Showing 1 - 10
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Nanoparticles of Lactiplantibacillus plantarum K8 Reduce Staphylococcus aureus Respiratory Infection and Tumor Necrosis Factor Alpha- and Interferon Gamma-Induced Lung Inflammation
Authors: Hong, J;Son, M;Sin, J;Kim, H;Chung, DK;
Nutrients
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Control
Luteolin ameliorates DSS-induced colitis in mice via suppressing macrophage activation and chemotaxis
Authors: Xue, L;Jin, X;Ji, T;Li, R;Zhuge, X;Xu, F;Quan, Z;Tong, H;Yu, W;
International immunopharmacology
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Inflammation of Dry Eye Syndrome: A Cellular Study of the Epithelial and Macrophagic Involvement of NFAT5 and RAGE
Authors: Henrioux, F;Navel, V;Belville, C;Charnay, C;Antoine, A;Chiambaretta, F;Sapin, V;Blanchon, L;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Prognostic impact and gene expression analysis of peri-tumoral alveolar macrophage in resected lung adenocarcinoma
Authors: Kagawa, Y;Nakai, T;Taki, T;Hashimoto, H;Tanaka, Y;Sakai, T;Shibata, Y;Izumi, H;Nosaki, K;Udagawa, H;Zenke, Y;Matsumoto, S;Yoh, K;Miyazaki, S;Watanabe, R;Kojima, M;Sakashita, S;Sakamoto, N;Tsuboi, M;Goto, K;Ishii, G;
Cancer science
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells
Authors: F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber
bioRxiv : the preprint server for biology, 2023-02-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function
Authors: Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan
PLoS ONE, 2023-01-20;18(1):e0280590.
Species: Human
Sample Types: Transfected Whole Cells
Applications: Bioassay, Surface Plasmon Resonance Micr
GPSM1 impairs metabolic homeostasis by controlling a pro-inflammatory pathway in macrophages
Authors: J Yan, Y Zhang, H Yu, Y Zong, D Wang, J Zheng, L Jin, X Yu, C Liu, Y Zhang, F Jiang, R Zhang, X Fang, T Xu, M Li, J Di, Y Lu, X Ma, J Zhang, W Jia, C Hu
Nature Communications, 2022-11-25;13(1):7260.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer
Authors: M Tobiume, A Mitsuhashi, A Saijo, H Ogino, T Afroj, H Ogawa, H Goto, S Sato, A Abe, K Haji, R Ozaki, H Takizawa, Y Nishioka
Oncology Letters, 2022-09-30;24(5):417.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Early Emergence of 5' Terminally Deleted Coxsackievirus-B3 RNA Forms Is Associated with Acute and Persistent Infections in Mouse Target Tissues
Authors: D Callon, AL Lebreil, N Bouland, C Fichel, P Fornès, L Andreolett, F Berri
Vaccines, 2022-07-28;10(8):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Chronic Low Dose Morphine Does Not Alter Two In Vitro BBB Models
Authors: J Marino, ME Maubert, JM Lawrence, B Wigdahl, MR Nonnemache
Brain sciences, 2022-07-06;12(7):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Inhibition of Vascular Endothelial Cadherin Cleavage Prevents Elastic Fiber Alterations and Atherosclerosis Induced by Intermittent Hypoxia in the Mouse Aorta
Authors: O Harki, S Bouyon, M Sallé, A Arco-Hierv, E Lemarié, A Demory, C Chirica, I Vilgrain, JL Pépin, G Faury, A Briançon-M
International Journal of Molecular Sciences, 2022-06-24;23(13):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

HMGA1 Promotes Macrophage Recruitment via Activation of NF-kappaB-CCL2 Signaling in Hepatocellular Carcinoma
Authors: J Chen, K Ji, L Gu, Y Fang, M Pan, S Tian
Oncogene, 2022-06-22;2022(0):4727198.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Immune Regulation of Mammary Fibroblasts and the Impact of Mammographic Density
Authors: M Archer, P Dasari, D Walsh, KL Britt, A Evdokiou, WV Ingman
Journal of Clinical Medicine, 2022-02-02;11(3):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Microglia-Derived Olfactomedin-like 3 Promotes Pro-Tumorigenic Microglial Function and Malignant Features of Glioma Cells
Authors: RG Toedebusch, CA Lucchesi, ET Debebe, LA Wittenburg, X Chen, CM Toedebusch
International Journal of Molecular Sciences, 2021-12-02;22(23):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors
Authors: L Déry, G Charest, B Guérin, M Akbari, D Fortin
International Journal of Molecular Sciences, 2021-11-10;22(22):.
Species: Human, Rat
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo

Cytokine profile and cytoskeletal changes after herpes simplex virus type 1 infection in human trabecular meshwork cells
Authors: JA Choi, HH Ju, JE Kim, J Lee, D Jee, CK Park, SY Paik
Journal of Cellular and Molecular Medicine, 2021-09-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Mimicking of Blood Flow Results in a Distinct Functional Phenotype in Human Non-Adherent Classical Monocytes
Authors: E Wirthgen, M Hornschuh, IM Wrobel, C Manteuffel, J Däbritz
Biology, 2021-08-04;10(8):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Role of MCP-1 and IL-8 in viral anterior uveitis, and contractility and fibrogenic activity of trabecular meshwork cells
Authors: J Lee, JA Choi, HH Ju, JE Kim, SY Paik, PV Rao
Scientific Reports, 2021-07-22;11(1):14950.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Melanoma Associated Chitinase 3-Like 1 Promoted Endothelial Cell Activation and Immune Cell Recruitment
Authors: G Ramos-Espi, Y Wang, JM Brandner, SW Schneider, C Gorzelanny
International Journal of Molecular Sciences, 2021-04-10;22(8):.
Species: Human
Sample Types: Protein
Applications: Bioassay

Hiltonol Cocktail Kills Lung Cancer Cells by Activating Cancer-Suppressors, PKR/OAS, and Restraining the Tumor Microenvironment
Authors: SC Chang, BX Zhang, EC Su, WC Wu, TH Hsieh, AM Salazar, YK Lin, JL Ding
International Journal of Molecular Sciences, 2021-02-05;22(4):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularization
Authors: X Liu, A Guo, Y Tu, W Li, L Li, W Liu, Y Ju, Y Zhou, A Sang, M Zhu
Cell Death Dis, 2020-11-27;11(11):1016.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Maresin 1 attenuates pro-inflammatory activation induced by &beta-amyloid and stimulates its uptake
Authors: Y Wang, A Leppert, S Tan, B van der Ga, N Li, M Schultzber, E Hjorth
J Cell Mol Med, 2020-11-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

CXCR4 inhibition in human pancreatic and colorectal cancers induces an integrated immune response
Authors: D Biasci, M Smoragiewi, CM Connell, Z Wang, Y Gao, JED Thaventhir, B Basu, L Magiera, TI Johnson, L Bax, A Gopinathan, C Isherwood, FA Gallagher, M Pawula, I Hudecova, D Gale, N Rosenfeld, P Barmpounak, EC Popa, R Brais, E Godfrey, F Mir, FM Richards, DT Fearon, T Janowitz, DI Jodrell
Proc Natl Acad Sci U S A, 2020-10-30;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culture
Authors: S Di Blasio, GF van Wigche, A Becker, A van Duffel, M Gorris, K Verrijp, I Stefanini, GJ Bakker, M Bloemendal, A Halilovic, A Vasaturo, G Bakdash, SV Hato, JHW de Wilt, J Schalkwijk, IJM de Vries, JC Textor, EH van den Bo, M Tazzari, CG Figdor
Nat Commun, 2020-06-02;11(1):2749.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Midkine activation of CD8+ T cells establishes a neuron-immune-cancer axis responsible for low-grade glioma growth
Authors: X Guo, Y Pan, M Xiong, S Sanapala, C Anastasaki, O Cobb, S Dahiya, DH Gutmann
Nat Commun, 2020-05-01;11(1):2177.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands
Authors: D Vreeken, CS Bruikman, SML Cox, H Zhang, R Lalai, A Koudijs, AJ van Zonnev, GK Hovingh, JM van Gils
J. Leukoc. Biol., 2020-04-26;0(0):.
Species: Human
Sample Types: Whole Cell
Applications: Cell Culture

Tanshinone IIA Alleviates CCL2-Induced Leaning memory and Cognition Impairment in Rats: A Potential Therapeutic Approach for HIV-Associated Neurocognitive Disorder
Authors: YJ Liao, JM Chen, JY Long, YJ Zhou, BY Liang, Y Zhou
Biomed Res Int, 2020-02-22;2020(0):2702175.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Wnt5a/CaMKII/ERK/CCL2 axis is required for tumor-associated macrophages to promote colorectal cancer progression
Authors: Q Liu, J Song, Y Pan, D Shi, C Yang, S Wang, B Xiong
Int. J. Biol. Sci., 2020-02-04;16(6):1023-1034.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Targeting FROUNT with disulfiram suppresses macrophage accumulation and its tumor-promoting properties
Authors: Y Terashima, E Toda, M Itakura, M Otsuji, S Yoshinaga, K Okumura, FHW Shand, Y Komohara, M Takeda, K Kokubo, MC Chen, S Yokoi, H Rokutan, Y Kofuku, K Ohnishi, M Ohira, T Iizasa, H Nakano, T Okabe, H Kojima, A Shimizu, S Kanegasaki, MR Zhang, I Shimada, H Nagase, H Terasawa, K Matsushima
Nat Commun, 2020-01-30;11(1):609.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Monocyte chemoattractant protein?1 promotes the proliferation, migration and differentiation potential of fibroblast?like synoviocytes via the PI3K/P38 cellular signaling pathway
Authors: X Tong, H Zeng, P Gu, K Wang, H Zhang, X Lin
Mol Med Rep, 2020-01-29;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture

PAK4 Kinase Activity Plays a Crucial Role in the Podosome Ring of Myeloid Cells
Authors: E Foxall, A Staszowska, LM Hirvonen, M Georgouli, M Ciccioli, A Rimmer, L Williams, Y Calle, VS Moreno, S Cox, GE Jones, CM Wells
Cell Rep, 2019-12-10;29(11):3385-3393.e6.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Circulating levels of ATP is a biomarker of HIV cognitive impairment
Authors: S Velasquez, L Prevedel, S Valdebenit, AM Gorska, M Golovko, N Khan, J Geiger, EA Eugenin
EBioMedicine, 2019-12-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CCL2 promotes macrophages-associated chemoresistance via MCPIP1 dual catalytic activities in multiple myeloma
Authors: R Xu, Y Li, H Yan, E Zhang, X Huang, Q Chen, J Chen, J Qu, Y Liu, J He, Q Yi, Z Cai
Cell Death Dis, 2019-10-14;10(10):781.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Placental chemokine compartmentalisation: A novel mammalian molecular control mechanism
Authors: KM Lee, GJ Wilson, M Pingen, A Fukuoka, CAH Hansell, R Bartolini, L Medina-Rui, GJ Graham
PLoS Biol., 2019-05-29;17(5):e3000287.
Species: Mouse, Transgenic Mouse
Sample Types: In Vivo
Applications: In Vivo

Effects of GADL1 overexpression on cell migration and the associated morphological changes
Authors: TN Wu, CK Chen, IC Liu, LS Wu, AT Cheng
Sci Rep, 2019-03-28;9(1):5298.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Intermittent Hypoxia Enhances THP-1 Monocyte Adhesion and Chemotaxis and Promotes M1 Macrophage Polarization via RAGE
Authors: J Zhou, W Bai, Q Liu, J Cui, W Zhang
Biomed Res Int, 2018-10-08;2018(0):1650456.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Long noncoding RNA LERFS negatively regulates rheumatoid synovial aggression and proliferation
Authors: Y Zou, S Xu, Y Xiao, Q Qiu, M Shi, J Wang, L Liang, Z Zhan, X Yang, N Olsen, SG Zheng, H Xu
J. Clin. Invest., 2018-09-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Estrogen Deficiency Promotes Cerebral Aneurysm Rupture by Upregulation of Th17 Cells and Interleukin-17A Which Downregulates E-Cadherin
Authors: BL Hoh, K Rojas, L Lin, HZ Fazal, S Hourani, KW Nowicki, MB Schneider, K Hosaka
J Am Heart Assoc, 2018-04-13;7(8):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Sulforaphane rescues amyloid-? peptide-mediated decrease in MerTK expression through its anti-inflammatory effect in human THP-1 macrophages
Authors: KA Jhang, JS Park, HS Kim, YH Chong
J Neuroinflammation, 2018-03-12;15(1):75.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C-C motif chemokine ligand 2 induces proliferation and prevents lipopolysaccharide-induced inflammatory responses in bovine mammary epithelial cells
Authors: C Yang, W Lim, H Bae, FW Bazer, G Song
J. Dairy Sci., 2018-02-14;0(0):.
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay

Decellularization and Solubilization of Porcine Liver for Use as a Substrate for Porcine Hepatocyte Culture: Method Optimization and Comparison
Authors: RE Coronado, M Somaraki-C, S Natesan, RJ Christy, JL Ong, GA Halff
Cell Transplant, 2017-12-01;26(12):1840-1854.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Matrix degradability controls multicellularity of 3D cell migration
Authors: B Trappmann, BM Baker, WJ Polacheck, CK Choi, JA Burdick, CS Chen
Nat Commun, 2017-08-29;8(1):371.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

miRNA-1246 induces pro-inflammatory responses in mesenchymal stem/stromal cells by regulating PKA and PP2A
Authors: A Bott, N Erdem, S Lerrer, A Hotz-Wagen, C Breunig, K Abnaof, A Wörner, H Wilhelm, E Münsterman, A Ben-Baruch, S Wiemann
Oncotarget, 2017-07-04;8(27):43897-43914.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

p115 RhoGEF activates the Rac1 GTPase signaling cascade in MCP1-induced vascular smooth muscle cell migration and proliferation
Authors: NK Singh, J Janjanam, GN Rao
J. Biol. Chem., 2017-06-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Antibody blockade of CLEC12A delays EAE onset and attenuates disease severity by impairing myeloid cell CNS infiltration and restoring positive immunity
Authors: D Sagar, NP Singh, R Ginwala, X Huang, R Philip, M Nagarkatti, P Nagarkatti, K Neumann, J Ruland, AM Andrews, SH Ramirez, ZK Khan, P Jain
Sci Rep, 2017-06-02;7(1):2707.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hyperglycemia-induced Renal P2X7 Receptor Activation Enhances Diabetes-related Injury
Authors: RI Menzies, JWR Booth, JJ Mullins, MA Bailey, FWK Tam, JT Norman, RJ Unwin
EBioMedicine, 2017-04-20;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA (Standard)

Secreted Ectodomain of SIGLEC-9 and MCP-1 Synergistically Improve Acute Liver Failure in Rats by Altering Macrophage Polarity
Authors: T Ito, M Ishigami, Y Matsushita, M Hirata, K Matsubara, T Ishikawa, H Hibi, M Ueda, Y Hirooka, H Goto, A Yamamoto
Sci Rep, 2017-03-08;7(0):44043.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Aberrant SYK kinase signaling is essential for tumorigenesis induced by TSC2 inactivation
Authors: Y Cui, WK Steagall, A Lamattina, G Pacheco-Ro, M Stylianou, P Kidambi, B Stump, F Golzarri, IO Rosas, C Priolo, EP Henske, J Moss, S El-Chemaly
Cancer Res, 2017-02-15;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases
Cell Death Dis, 2016-12-29;7(12):e2565.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Macrophages Enhance Migration in Inflammatory Breast Cancer Cells via RhoC GTPase Signaling
Sci Rep, 2016-12-19;6(0):39190.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study
Authors: Benjamin Malard
Artif Organs, 2016-12-02;0(0):.
Species: Human
Sample Types: Plasma
Applications: Hemofiltration Procedure

Cytokine-Like 1 Regulates Cardiac Fibrosis via Modulation of TGF-? Signaling
PLoS ONE, 2016-11-11;11(11):e0166480.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

PARP inhibition in leukocytes diminishes inflammation via effects on integrins/cytoskeleton and protects the blood-brain barrier
J Neuroinflammation, 2016-09-27;13(1):254.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

The Effect of MCP-1/CCR2 on the Proliferation and Senescence of Epidermal Constituent Cells in Solar Lentigo
Authors: Woo Jin Lee
Int J Mol Sci, 2016-06-15;17(6):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression
Authors: RG de Bruin, L Shiue, J Prins, HC de Boer, A Singh, WS Fagg, JM van Gils, JM Duijs, S Katzman, AO Kraaijevel, S Böhringer, WY Leung, SM Kielbasa, JP Donahue, PH van der Za, R Sijbom, CM van Alem, I Bot, C van Kooten, JW Jukema, H Van Esch, T Rabelink
Nat Commun, 2016-03-31;7(0):10846.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Serum chemokine (CC motif) ligand 2 level as a diagnostic, predictive, and prognostic biomarker for prostate cancer.
Authors: Kouji Izumi, Atsushi Mizokami, Hsiu-Ping Lin, Hui-Min Ho, Hiroaki Iwamoto, Aerken Maolake, Ariunbold Natsagdor, Yasuhide Kitagawa, Yoshifumi Kadono, Hiroshi Miyamoto, Chiung-Kuei Huang, Mikio Namiki, Wen-Jye Lin
Oncotarget, 2016-02-16;0(0):1949-2553.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Long-acting progestin-only contraceptives impair endometrial vasculature by inhibiting uterine vascular smooth muscle cell survival.
Authors: Kayisli U, Basar M, Guzeloglu-Kayisli O, Semerci N, Atkinson H, Shapiro J, Summerfield T, Huang S, Prelle K, Schatz F, Lockwood C
Proc Natl Acad Sci U S A, 2015-04-06;112(16):5153-8.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Mesenchymal stem/stromal cells protect against autoimmunity via CCL2-dependent recruitment of myeloid-derived suppressor cells.
Authors: Lee H, Ko J, Jeong H, Ko A, Kim M, Wee W, Yoon S, Oh J
J Immunol, 2015-03-13;194(8):3634-45.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Buprenorphine decreases the CCL2-mediated chemotactic response of monocytes.
Authors: Carvallo L, Lopez L, Che F, Lim J, Eugenin E, Williams D, Nieves E, Calderon T, Madrid-Aliste C, Fiser A, Weiss L, Angeletti R, Berman J
J Immunol, 2015-02-25;194(7):3246-58.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S100A9 induced inflammatory responses are mediated by distinct damage associated molecular patterns (DAMP) receptors in vitro and in vivo.
Authors: Chen, Bo, Miller, Allison, Rebelatto, Marlon, Brewah, Yambasu, Rowe, Daniel C, Clarke, Lori, Czapiga, Meggan, Rosenthal, Kim, Imamichi, Tomozumi, Chen, Yan, Chang, Chew-Shu, Chowdhury, Partha S, Naiman, Brian, Wang, Yue, Yang, De, Humbles, Alison A, Herbst, Ronald, Sims, Gary P
PLoS ONE, 2015-02-23;10(2):e0115828.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells.
Authors: Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M
Invest Ophthalmol Vis Sci, 2015-01-20;56(2):1051-62.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Chemokine-coupled beta2 integrin-induced macrophage Rac2-Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis.
Authors: Morrison A, Yarovinsky T, Young B, Moraes F, Ross T, Ceneri N, Zhang J, Zhuang Z, Sinusas A, Pardi R, Schwartz M, Simons M, Bender J
J Exp Med, 2014-09-01;211(10):1957-68.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Technical advance: live-imaging analysis of human dendritic cell migrating behavior under the influence of immune-stimulating reagents in an organotypic model of lung.
Authors: Nguyen Hoang A, Chen P, Bjornfot S, Hogstrand K, Lock J, Grandien A, Coles M, Svensson M
J Leukoc Biol, 2014-06-04;96(3):481-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Characterization of platelet-monocyte complexes in HIV-1-infected individuals: possible role in HIV-associated neuroinflammation.
Authors: Singh, Meera V, Davidson, Donna C, Jackson, Joseph W, Singh, Vir B, Silva, Jharon, Ramirez, Servio H, Maggirwar, Sanjay B
J Immunol, 2014-04-11;192(10):4674-84.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.
Authors: Italiani, Paola, Mazza, Emilia M, Lucchesi, Davide, Cifola, Ingrid, Gemelli, Claudia, Grande, Alexis, Battaglia, Cristina, Bicciato, Silvio, Boraschi, Diana
PLoS ONE, 2014-02-03;9(2):e87680.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CD14(hi)HLA-DR(dim) macrophages, with a resemblance to classical blood monocytes, dominate inflamed mucosa in Crohn's disease.
Authors: Thiesen S, Janciauskiene S, Uronen-Hansson H, Agace W, Hogerkorp C, Spee P, Hakansson K, Grip O
J Leukoc Biol, 2013-11-08;95(3):531-41.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Novel role for p21-activated kinase 2 in thrombin-induced monocyte migration.
Authors: Gadepalli R, Kotla S, Heckle M, Verma S, Singh N, Rao G
J Biol Chem, 2013-09-11;288(43):30815-31.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Immunogenic calreticulin exposure occurs through a phylogenetically conserved stress pathway involving the chemokine CXCL8.
Authors: Sukkurwala A, Martins I, Wang Y, Schlemmer F, Ruckenstuhl C, Durchschlag M, Michaud M, Senovilla L, Sistigu A, Ma Y, Vacchelli E, Sulpice E, Gidrol X, Zitvogel L, Madeo F, Galluzzi L, Kepp O, Kroemer G
Cell Death Differ, 2013-06-21;21(1):59-68.

Nuclear factor of activated T cells c1 mediates p21-activated kinase 1 activation in the modulation of chemokine-induced human aortic smooth muscle cell F-actin stress fiber formation, migration, and proliferation and injury-induced vascular wall remodeling.
Authors: Kundumani-Sridharan, Venkates, Singh, Nikhlesh, Kumar, Sanjay, Gadepalli, Ravisekh, Rao, Gadipart
J Biol Chem, 2013-06-04;288(30):22150-62.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

The novel role of IL-7 ligation to IL-7 receptor in myeloid cells of rheumatoid arthritis and collagen-induced arthritis.
Authors: Chen Z, Kim S, Chamberlain N, Pickens S, Volin M, Volkov S, Arami S, Christman J, Prabhakar B, Swedler W, Mehta A, Sweiss N, Shahrara S
J Immunol, 2013-04-19;190(10):5256-66.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
Authors: van Gils, Janine M, Ramkhelawon, Bhama, Fernandes, Luciana, Stewart, Merran C, Guo, Liang, Seibert, Tara, Menezes, Gustavo, Cara, Denise C, Chow, Camille, Kinane, T Bernar, Fisher, Edward A, Balcells, Mercedes, Alvarez-Leite, Jacqueli, Lacy-Hulbert, Adam, Moore, Kathryn
Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):911-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Xanthine oxidoreductase promotes the inflammatory state of mononuclear phagocytes through effects on chemokine expression, peroxisome proliferator-activated receptor-gamma sumoylation, and HIF-1alpha.
Authors: Gibbings S, Elkins ND, Fitzgerald H, Tiao J, Weyman ME, Shibao G, Fini MA, Wright RM
J. Biol. Chem., 2010-11-08;286(2):961-75.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Enhanced tumor trafficking of GD2 chimeric antigen receptor T cells by expression of the chemokine receptor CCR2b.
Authors: Craddock J, Lu A, Bear A, Pule M, Brenner M, Rooney C, Foster A
J Immunother, 2010-10-01;33(8):780-8.
Species: Human
Sample Types: Whole Cells
Applications: CAR-T (Bioassay)

A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010-06-08;56(0):1320.
Applications: ELISA (Standard)

IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
Authors: Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
J. Immunol., 2009-12-21;184(3):1642-52.
Species: Human
Sample Types: Carbohydrates
Applications: Bioassay

CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B.
Authors: Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M
Immunology, 2009-12-02;129(4):536-46.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

IL-17 induces monocyte migration in rheumatoid arthritis.
Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
J. Immunol., 2009-03-15;182(6):3884-91.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
J. Immunol., 2008-12-01;181(11):8053-67.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
J. Leukoc. Biol., 2008-06-30;84(4):1109-19.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
Authors: Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
Blood, 2008-05-14;112(3):493-503.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes.
Authors: Burt D, Salvidio G, Tarabra E, Barutta F, Pinach S, Dentelli P, Camussi G, Perin PC, Gruden G
Am. J. Pathol., 2007-11-30;171(6):1789-99.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC.
Authors: Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T
Arterioscler. Thromb. Vasc. Biol., 2007-11-08;28(1):135-41.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clin. Chem., 2007-11-01;53(11):2010-2.
Applications: ELISA (Standard)

Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007-10-22;329(1):214-8.
Applications: ELISA (Standard)

Ultrasensitive flow-based immunoassays using single-molecule counting.
Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clin. Chem., 2007-09-21;53(11):1990-5.
Applications: ELISA (Standard)

Tumor-derived chemokine MCP-1/CCL2 is sufficient for mediating tumor tropism of adoptively transferred T cells.
Authors: Brown CE, Vishwanath RP, Aguilar B, Starr R, Najbauer J, Aboody KS, Jensen MC
J. Immunol., 2007-09-01;179(5):3332-41.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
Biochem. Biophys. Res. Commun., 2007-05-02;358(2):528-33.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CD16+ monocyte-derived macrophages activate resting T cells for HIV infection by producing CCR3 and CCR4 ligands.
Authors: Ancuta P, Autissier P, Wurcel A, Zaman T, Stone D, Gabuzda D
J. Immunol., 2006-05-15;176(10):5760-71.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Novel antiviral activity of chemokines.
Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
Virology, 2006-04-17;350(2):484-92.
Species: Virus
Sample Types: Virus, Whole Cells
Applications: Binding Assay, Bioassay

Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation.
Authors: Nguyen AN, Stebbins EG, Henson M, O&apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS
Exp. Cell Res., 2006-04-04;312(10):1909-23.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

HIV-1 tat protein induces a migratory phenotype in human fetal microglia by a CCL2 (MCP-1)-dependent mechanism: possible role in NeuroAIDS.
Authors: Eugenin EA, Dyer G, Calderon TM, Berman JW
Glia, 2005-03-01;49(4):501-10.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
J. Immunol., 2004-12-01;173(11):6592-602.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

trans fatty acids and systemic inflammation in heart failure.
Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
Applications: ELISA (Standard)

Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2.
Authors: Traves SL, Smith SJ, Barnes PJ, Donnelly LE
J. Leukoc. Biol., 2004-05-20;76(2):441-50.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
Blood, 2004-03-25;104(2):314-20.
Applications: ELISA (Standard)

Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain.
Authors: Banisadr G, Queraud-Lesaux F, Boutterin MC, Pelaprat D, Zalc B, Rostene W, Haour F, Parsadaniantz SM
J. Neurochem., 2002-04-01;81(2):257-69.
Species: Rat
Sample Types: Whole Tissue
Applications: Binding Assay

FAQs

Should I use Recombinant Human CCL2/MCP-1 Protein formulated with carrier BSA (Catalog # 279-MC) or the Recombinant Human CCL2/MCP-1 Protein (Catalog # 279-MC/CF) that is carrier-free (no BSA) for my in vivo experiments?
- For in vivo aplications, we would recommend using the carrier-free Recombinant Human CCL2/MCP-1 Protein (Catalog # 279-MC/CF). The injection of a carrier protein such as BSA can cause side effects and sometimes death to the experimental animal. For more information regarding use of the protein for in vivo applications, we would recommend consulting the Citations tab on the product-specific page.

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