Home / CCL2/JE/MCP-1 / Recombinant Human CCL2/MCP-1 Protein

Recombinant Human CCL2/MCP-1 Protein

Catalog #: 279-MC
98 Citations 10 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
279-MC-001MG/CF
279-MC-010/CF
279-MC-050/CF

With Carrier

Catalog # Availability Size / Price Qty
279-MC-010
279-MC-050
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Recombinant Human CCL2/MCP-1 Protein Bioactivity
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Product Details
Citations (98)
FAQs
Reviews (10)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CCL2/MCP-1 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED50 for this effect is 5‑30 ng/mL.
Source
E. coli-derived human CCL2/JE/MCP-1 protein
Gln24-Thr99
Accession #
P13500.1
N-terminal Sequence
Analysis
Gln24
Predicted Molecular Mass
8.7 kDa
SDS-PAGE
10 kDa, reducing conditions

Product Datasheets

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279-MC (with carrier)

Product Datasheet
COA
SDS

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279-MC/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

279-MC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

279-MC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human CCL2/MCP-1 Protein Bioactivity View Larger

Recombinant Human CCL2/ MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A. The ED50 for this effect is 5-30 ng/mL.

SDS-PAGE Recombinant Human CCL2/MCP-1 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human CCL2/MCP-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 10 kDa.

Mass Spectrometry Recombinant Human CCL2/MCP-1 Protein Mass Spectrometry View Larger

MALDI-TOF analysis of Recombinant Human CCL2/MCP-1. The major peak corresponds to the calculated molecular mass, 8679 Da. The minor peak at 8887 is a matrix-associated artifact of the MALDI-TOF.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL2/JE/MCP-1

CCL2, also called monocyte chemotactic protein‑1 (MCP‑1) or JE, is a member of the C‑C or beta  chemokine family that is best known as a chemotactic agent for mononuclear cells (1, 2). Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a 23 aa signal peptide and a 76 aa mature protein (2). Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acid‑modified glutamine, occurs naturally by metalloproteinase cleavage and down‑regulates activity but not receptor binding (3). CCL2 may form multiple bands from 8.7‑13.5 kDa on SDS‑PAGE due to non‑covalent dimerization and variable carbohydrate content (3). Mature human CCL2 shares 78‑79% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 aa and shares only ~56% aa identity within the common region. Human CCL2 can, however, induce a response in murine cells (4). Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source (1, 2). In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor, CCR2 (2). Additionally, it promotes Th2 polarization in CD4+ T cells (5). CCL2‑mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma (6, 7).

References
  1. Yoshimura, T. et al. (1989) FEBS Lett. 244:487.
  2. Deshmane, S.L. et al. (2009) J. Interferon Cytokine Res. 29:313.
  3. Proost, P. et al.(1998) J. Immunol. 160:4034.
  4. Ju Lee, H. et al. (2015) J. Immunol. 194:3634.
  5. Luther, S.A. and J.G. Cyster (2001) Nat. Immunol. 2:102.
  6. Daly, C. et al. (2003) Microcirculation 10:247.
  7. Aukrust, P. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1909.
    Entrez Gene IDs
    6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
    Alternate Names
    C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

    Citations for Recombinant Human CCL2/MCP-1 Protein

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    98 Citations: Showing 1 - 10
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    1. CD14+CD16+ monocyte transmigration across the blood-brain barrier is associated with HIV-NCI despite viral suppression
      Authors: Veksler, V;Leon-Rivera, R;Fleysher, L;Gonzalez, J;Lopez, JA;Rubin, LH;Morgello, S;Berman, JW;
      JCI insight
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    2. Nanoparticles of Lactiplantibacillus plantarum K8 Reduce Staphylococcus aureus Respiratory Infection and Tumor Necrosis Factor Alpha- and Interferon Gamma-Induced Lung Inflammation
      Authors: Hong, J;Son, M;Sin, J;Kim, H;Chung, DK;
      Nutrients
      Species: Human
      Sample Types: Cell Culture Supernates
      Applications: ELISA Control
    3. Luteolin ameliorates DSS-induced colitis in mice via suppressing macrophage activation and chemotaxis
      Authors: Xue, L;Jin, X;Ji, T;Li, R;Zhuge, X;Xu, F;Quan, Z;Tong, H;Yu, W;
      International immunopharmacology
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Bioassay
    4. Inflammation of Dry Eye Syndrome: A Cellular Study of the Epithelial and Macrophagic Involvement of NFAT5 and RAGE
      Authors: Henrioux, F;Navel, V;Belville, C;Charnay, C;Antoine, A;Chiambaretta, F;Sapin, V;Blanchon, L;
      International journal of molecular sciences
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    5. Prognostic impact and gene expression analysis of peri-tumoral alveolar macrophage in resected lung adenocarcinoma
      Authors: Kagawa, Y;Nakai, T;Taki, T;Hashimoto, H;Tanaka, Y;Sakai, T;Shibata, Y;Izumi, H;Nosaki, K;Udagawa, H;Zenke, Y;Matsumoto, S;Yoh, K;Miyazaki, S;Watanabe, R;Kojima, M;Sakashita, S;Sakamoto, N;Tsuboi, M;Goto, K;Ishii, G;
      Cancer science
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    6. Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells
      Authors: F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber
      bioRxiv : the preprint server for biology, 2023-02-13;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    7. Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function
      Authors: Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan
      PLoS ONE, 2023-01-20;18(1):e0280590.
      Species: Human
      Sample Types: Transfected Whole Cells
      Applications: Bioassay, Surface Plasmon Resonance Micr
    8. GPSM1 impairs metabolic homeostasis by controlling a pro-inflammatory pathway in macrophages
      Authors: J Yan, Y Zhang, H Yu, Y Zong, D Wang, J Zheng, L Jin, X Yu, C Liu, Y Zhang, F Jiang, R Zhang, X Fang, T Xu, M Li, J Di, Y Lu, X Ma, J Zhang, W Jia, C Hu
      Nature Communications, 2022-11-25;13(1):7260.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    9. Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer
      Authors: M Tobiume, A Mitsuhashi, A Saijo, H Ogino, T Afroj, H Ogawa, H Goto, S Sato, A Abe, K Haji, R Ozaki, H Takizawa, Y Nishioka
      Oncology Letters, 2022-09-30;24(5):417.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    10. Early Emergence of 5' Terminally Deleted Coxsackievirus-B3 RNA Forms Is Associated with Acute and Persistent Infections in Mouse Target Tissues
      Authors: D Callon, AL Lebreil, N Bouland, C Fichel, P Fornès, L Andreolett, F Berri
      Vaccines, 2022-07-28;10(8):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    11. Chronic Low Dose Morphine Does Not Alter Two In Vitro BBB Models
      Authors: J Marino, ME Maubert, JM Lawrence, B Wigdahl, MR Nonnemache
      Brain sciences, 2022-07-06;12(7):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    12. Inhibition of Vascular Endothelial Cadherin Cleavage Prevents Elastic Fiber Alterations and Atherosclerosis Induced by Intermittent Hypoxia in the Mouse Aorta
      Authors: O Harki, S Bouyon, M Sallé, A Arco-Hierv, E Lemarié, A Demory, C Chirica, I Vilgrain, JL Pépin, G Faury, A Briançon-M
      International Journal of Molecular Sciences, 2022-06-24;23(13):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    13. HMGA1 Promotes Macrophage Recruitment via Activation of NF-kappaB-CCL2 Signaling in Hepatocellular Carcinoma
      Authors: J Chen, K Ji, L Gu, Y Fang, M Pan, S Tian
      Oncogene, 2022-06-22;2022(0):4727198.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    14. Immune Regulation of Mammary Fibroblasts and the Impact of Mammographic Density
      Authors: M Archer, P Dasari, D Walsh, KL Britt, A Evdokiou, WV Ingman
      Journal of Clinical Medicine, 2022-02-02;11(3):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    15. Microglia-Derived Olfactomedin-like 3 Promotes Pro-Tumorigenic Microglial Function and Malignant Features of Glioma Cells
      Authors: RG Toedebusch, CA Lucchesi, ET Debebe, LA Wittenburg, X Chen, CM Toedebusch
      International Journal of Molecular Sciences, 2021-12-02;22(23):.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Bioassay
    16. Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors
      Authors: L Déry, G Charest, B Guérin, M Akbari, D Fortin
      International Journal of Molecular Sciences, 2021-11-10;22(22):.
      Species: Human, Rat
      Sample Types: In Vivo, Whole Cells
      Applications: Bioassay, In Vivo
    17. Cytokine profile and cytoskeletal changes after herpes simplex virus type 1 infection in human trabecular meshwork cells
      Authors: JA Choi, HH Ju, JE Kim, J Lee, D Jee, CK Park, SY Paik
      Journal of Cellular and Molecular Medicine, 2021-09-01;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    18. Mimicking of Blood Flow Results in a Distinct Functional Phenotype in Human Non-Adherent Classical Monocytes
      Authors: E Wirthgen, M Hornschuh, IM Wrobel, C Manteuffel, J Däbritz
      Biology, 2021-08-04;10(8):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    19. Role of MCP-1 and IL-8 in viral anterior uveitis, and contractility and fibrogenic activity of trabecular meshwork cells
      Authors: J Lee, JA Choi, HH Ju, JE Kim, SY Paik, PV Rao
      Scientific Reports, 2021-07-22;11(1):14950.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    20. Melanoma Associated Chitinase 3-Like 1 Promoted Endothelial Cell Activation and Immune Cell Recruitment
      Authors: G Ramos-Espi, Y Wang, JM Brandner, SW Schneider, C Gorzelanny
      International Journal of Molecular Sciences, 2021-04-10;22(8):.
      Species: Human
      Sample Types: Protein
      Applications: Bioassay
    21. Hiltonol Cocktail Kills Lung Cancer Cells by Activating Cancer-Suppressors, PKR/OAS, and Restraining the Tumor Microenvironment
      Authors: SC Chang, BX Zhang, EC Su, WC Wu, TH Hsieh, AM Salazar, YK Lin, JL Ding
      International Journal of Molecular Sciences, 2021-02-05;22(4):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    22. Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularization
      Authors: X Liu, A Guo, Y Tu, W Li, L Li, W Liu, Y Ju, Y Zhou, A Sang, M Zhu
      Cell Death Dis, 2020-11-27;11(11):1016.
      Species: Human
      Sample Types: Whole Cells
      Applications: Cell Culture
    23. Maresin 1 attenuates pro-inflammatory activation induced by &beta-amyloid and stimulates its uptake
      Authors: Y Wang, A Leppert, S Tan, B van der Ga, N Li, M Schultzber, E Hjorth
      J Cell Mol Med, 2020-11-22;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Cell Culture
    24. CXCR4 inhibition in human pancreatic and colorectal cancers induces an integrated immune response
      Authors: D Biasci, M Smoragiewi, CM Connell, Z Wang, Y Gao, JED Thaventhir, B Basu, L Magiera, TI Johnson, L Bax, A Gopinathan, C Isherwood, FA Gallagher, M Pawula, I Hudecova, D Gale, N Rosenfeld, P Barmpounak, EC Popa, R Brais, E Godfrey, F Mir, FM Richards, DT Fearon, T Janowitz, DI Jodrell
      Proc Natl Acad Sci U S A, 2020-10-30;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    25. The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culture
      Authors: S Di Blasio, GF van Wigche, A Becker, A van Duffel, M Gorris, K Verrijp, I Stefanini, GJ Bakker, M Bloemendal, A Halilovic, A Vasaturo, G Bakdash, SV Hato, JHW de Wilt, J Schalkwijk, IJM de Vries, JC Textor, EH van den Bo, M Tazzari, CG Figdor
      Nat Commun, 2020-06-02;11(1):2749.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Bioassay
    26. Midkine activation of CD8+ T cells establishes a neuron-immune-cancer axis responsible for low-grade glioma growth
      Authors: X Guo, Y Pan, M Xiong, S Sanapala, C Anastasaki, O Cobb, S Dahiya, DH Gutmann
      Nat Commun, 2020-05-01;11(1):2177.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Cell Culture
    27. EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands
      Authors: D Vreeken, CS Bruikman, SML Cox, H Zhang, R Lalai, A Koudijs, AJ van Zonnev, GK Hovingh, JM van Gils
      J. Leukoc. Biol., 2020-04-26;0(0):.
      Species: Human
      Sample Types: Whole Cell
      Applications: Cell Culture
    28. Tanshinone IIA Alleviates CCL2-Induced Leaning memory and Cognition Impairment in Rats: A Potential Therapeutic Approach for HIV-Associated Neurocognitive Disorder
      Authors: YJ Liao, JM Chen, JY Long, YJ Zhou, BY Liang, Y Zhou
      Biomed Res Int, 2020-02-22;2020(0):2702175.
      Species: Rat
      Sample Types: In Vivo
      Applications: In Vivo
    29. Wnt5a/CaMKII/ERK/CCL2 axis is required for tumor-associated macrophages to promote colorectal cancer progression
      Authors: Q Liu, J Song, Y Pan, D Shi, C Yang, S Wang, B Xiong
      Int. J. Biol. Sci., 2020-02-04;16(6):1023-1034.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    30. Targeting FROUNT with disulfiram suppresses macrophage accumulation and its tumor-promoting properties
      Authors: Y Terashima, E Toda, M Itakura, M Otsuji, S Yoshinaga, K Okumura, FHW Shand, Y Komohara, M Takeda, K Kokubo, MC Chen, S Yokoi, H Rokutan, Y Kofuku, K Ohnishi, M Ohira, T Iizasa, H Nakano, T Okabe, H Kojima, A Shimizu, S Kanegasaki, MR Zhang, I Shimada, H Nagase, H Terasawa, K Matsushima
      Nat Commun, 2020-01-30;11(1):609.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Bioassay
    31. Monocyte chemoattractant protein?1 promotes the proliferation, migration and differentiation potential of fibroblast?like synoviocytes via the PI3K/P38 cellular signaling pathway
      Authors: X Tong, H Zeng, P Gu, K Wang, H Zhang, X Lin
      Mol Med Rep, 2020-01-29;0(0):.
      Species: Rat
      Sample Types: Whole Cells
      Applications: Cell Culture
    32. PAK4 Kinase Activity Plays a Crucial Role in the Podosome Ring of Myeloid Cells
      Authors: E Foxall, A Staszowska, LM Hirvonen, M Georgouli, M Ciccioli, A Rimmer, L Williams, Y Calle, VS Moreno, S Cox, GE Jones, CM Wells
      Cell Rep, 2019-12-10;29(11):3385-3393.e6.
      Species: Human
      Sample Types: Whole Cells
      Applications: Cell Culture
    33. Circulating levels of ATP is a biomarker of HIV cognitive impairment
      Authors: S Velasquez, L Prevedel, S Valdebenit, AM Gorska, M Golovko, N Khan, J Geiger, EA Eugenin
      EBioMedicine, 2019-12-03;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    34. CCL2 promotes macrophages-associated chemoresistance via MCPIP1 dual catalytic activities in multiple myeloma
      Authors: R Xu, Y Li, H Yan, E Zhang, X Huang, Q Chen, J Chen, J Qu, Y Liu, J He, Q Yi, Z Cai
      Cell Death Dis, 2019-10-14;10(10):781.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    35. Placental chemokine compartmentalisation: A novel mammalian molecular control mechanism
      Authors: KM Lee, GJ Wilson, M Pingen, A Fukuoka, CAH Hansell, R Bartolini, L Medina-Rui, GJ Graham
      PLoS Biol., 2019-05-29;17(5):e3000287.
      Species: Mouse, Transgenic Mouse
      Sample Types: In Vivo
      Applications: In Vivo
    36. Effects of GADL1 overexpression on cell migration and the associated morphological changes
      Authors: TN Wu, CK Chen, IC Liu, LS Wu, AT Cheng
      Sci Rep, 2019-03-28;9(1):5298.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    37. Intermittent Hypoxia Enhances THP-1 Monocyte Adhesion and Chemotaxis and Promotes M1 Macrophage Polarization via RAGE
      Authors: J Zhou, W Bai, Q Liu, J Cui, W Zhang
      Biomed Res Int, 2018-10-08;2018(0):1650456.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    38. Long noncoding RNA LERFS negatively regulates rheumatoid synovial aggression and proliferation
      Authors: Y Zou, S Xu, Y Xiao, Q Qiu, M Shi, J Wang, L Liang, Z Zhan, X Yang, N Olsen, SG Zheng, H Xu
      J. Clin. Invest., 2018-09-10;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    39. Estrogen Deficiency Promotes Cerebral Aneurysm Rupture by Upregulation of Th17 Cells and Interleukin-17A Which Downregulates E-Cadherin
      Authors: BL Hoh, K Rojas, L Lin, HZ Fazal, S Hourani, KW Nowicki, MB Schneider, K Hosaka
      J Am Heart Assoc, 2018-04-13;7(8):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    40. Sulforaphane rescues amyloid-? peptide-mediated decrease in MerTK expression through its anti-inflammatory effect in human THP-1 macrophages
      Authors: KA Jhang, JS Park, HS Kim, YH Chong
      J Neuroinflammation, 2018-03-12;15(1):75.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    41. C-C motif chemokine ligand 2 induces proliferation and prevents lipopolysaccharide-induced inflammatory responses in bovine mammary epithelial cells
      Authors: C Yang, W Lim, H Bae, FW Bazer, G Song
      J. Dairy Sci., 2018-02-14;0(0):.
      Species: Bovine
      Sample Types: Whole Cells
      Applications: Bioassay
    42. Decellularization and Solubilization of Porcine Liver for Use as a Substrate for Porcine Hepatocyte Culture: Method Optimization and Comparison
      Authors: RE Coronado, M Somaraki-C, S Natesan, RJ Christy, JL Ong, GA Halff
      Cell Transplant, 2017-12-01;26(12):1840-1854.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    43. Matrix degradability controls multicellularity of 3D cell migration
      Authors: B Trappmann, BM Baker, WJ Polacheck, CK Choi, JA Burdick, CS Chen
      Nat Commun, 2017-08-29;8(1):371.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    44. miRNA-1246 induces pro-inflammatory responses in mesenchymal stem/stromal cells by regulating PKA and PP2A
      Authors: A Bott, N Erdem, S Lerrer, A Hotz-Wagen, C Breunig, K Abnaof, A Wörner, H Wilhelm, E Münsterman, A Ben-Baruch, S Wiemann
      Oncotarget, 2017-07-04;8(27):43897-43914.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    45. p115 RhoGEF activates the Rac1 GTPase signaling cascade in MCP1-induced vascular smooth muscle cell migration and proliferation
      Authors: NK Singh, J Janjanam, GN Rao
      J. Biol. Chem., 2017-06-27;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    46. Antibody blockade of CLEC12A delays EAE onset and attenuates disease severity by impairing myeloid cell CNS infiltration and restoring positive immunity
      Authors: D Sagar, NP Singh, R Ginwala, X Huang, R Philip, M Nagarkatti, P Nagarkatti, K Neumann, J Ruland, AM Andrews, SH Ramirez, ZK Khan, P Jain
      Sci Rep, 2017-06-02;7(1):2707.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    47. Hyperglycemia-induced Renal P2X7 Receptor Activation Enhances Diabetes-related Injury
      Authors: RI Menzies, JWR Booth, JJ Mullins, MA Bailey, FWK Tam, JT Norman, RJ Unwin
      EBioMedicine, 2017-04-20;0(0):.
      Species: Human
      Sample Types: Cell Culture Supernates
      Applications: ELISA (Standard)
    48. Secreted Ectodomain of SIGLEC-9 and MCP-1 Synergistically Improve Acute Liver Failure in Rats by Altering Macrophage Polarity
      Authors: T Ito, M Ishigami, Y Matsushita, M Hirata, K Matsubara, T Ishikawa, H Hibi, M Ueda, Y Hirooka, H Goto, A Yamamoto
      Sci Rep, 2017-03-08;7(0):44043.
      Species: Rat
      Sample Types: In Vivo
      Applications: In Vivo
    49. Aberrant SYK kinase signaling is essential for tumorigenesis induced by TSC2 inactivation
      Authors: Y Cui, WK Steagall, A Lamattina, G Pacheco-Ro, M Stylianou, P Kidambi, B Stump, F Golzarri, IO Rosas, C Priolo, EP Henske, J Moss, S El-Chemaly
      Cancer Res, 2017-02-15;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    50. Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases
      Cell Death Dis, 2016-12-29;7(12):e2565.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    51. Macrophages Enhance Migration in Inflammatory Breast Cancer Cells via RhoC GTPase Signaling
      Sci Rep, 2016-12-19;6(0):39190.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    52. Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study
      Authors: Benjamin Malard
      Artif Organs, 2016-12-02;0(0):.
      Species: Human
      Sample Types: Plasma
      Applications: Hemofiltration Procedure
    53. Cytokine-Like 1 Regulates Cardiac Fibrosis via Modulation of TGF-? Signaling
      PLoS ONE, 2016-11-11;11(11):e0166480.
      Species: Rat
      Sample Types: Whole Cells
      Applications: Bioassay
    54. PARP inhibition in leukocytes diminishes inflammation via effects on integrins/cytoskeleton and protects the blood-brain barrier
      J Neuroinflammation, 2016-09-27;13(1):254.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    55. The Effect of MCP-1/CCR2 on the Proliferation and Senescence of Epidermal Constituent Cells in Solar Lentigo
      Authors: Woo Jin Lee
      Int J Mol Sci, 2016-06-15;17(6):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    56. Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression
      Authors: RG de Bruin, L Shiue, J Prins, HC de Boer, A Singh, WS Fagg, JM van Gils, JM Duijs, S Katzman, AO Kraaijevel, S Böhringer, WY Leung, SM Kielbasa, JP Donahue, PH van der Za, R Sijbom, CM van Alem, I Bot, C van Kooten, JW Jukema, H Van Esch, T Rabelink
      Nat Commun, 2016-03-31;7(0):10846.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    57. Serum chemokine (CC motif) ligand 2 level as a diagnostic, predictive, and prognostic biomarker for prostate cancer.
      Authors: Kouji Izumi, Atsushi Mizokami, Hsiu-Ping Lin, Hui-Min Ho, Hiroaki Iwamoto, Aerken Maolake, Ariunbold Natsagdor, Yasuhide Kitagawa, Yoshifumi Kadono, Hiroshi Miyamoto, Chiung-Kuei Huang, Mikio Namiki, Wen-Jye Lin
      Oncotarget, 2016-02-16;0(0):1949-2553.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    58. Long-acting progestin-only contraceptives impair endometrial vasculature by inhibiting uterine vascular smooth muscle cell survival.
      Authors: Kayisli U, Basar M, Guzeloglu-Kayisli O, Semerci N, Atkinson H, Shapiro J, Summerfield T, Huang S, Prelle K, Schatz F, Lockwood C
      Proc Natl Acad Sci U S A, 2015-04-06;112(16):5153-8.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    59. Mesenchymal stem/stromal cells protect against autoimmunity via CCL2-dependent recruitment of myeloid-derived suppressor cells.
      Authors: Lee H, Ko J, Jeong H, Ko A, Kim M, Wee W, Yoon S, Oh J
      J Immunol, 2015-03-13;194(8):3634-45.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Bioassay
    60. Buprenorphine decreases the CCL2-mediated chemotactic response of monocytes.
      Authors: Carvallo L, Lopez L, Che F, Lim J, Eugenin E, Williams D, Nieves E, Calderon T, Madrid-Aliste C, Fiser A, Weiss L, Angeletti R, Berman J
      J Immunol, 2015-02-25;194(7):3246-58.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    61. S100A9 induced inflammatory responses are mediated by distinct damage associated molecular patterns (DAMP) receptors in vitro and in vivo.
      Authors: Chen, Bo, Miller, Allison, Rebelatto, Marlon, Brewah, Yambasu, Rowe, Daniel C, Clarke, Lori, Czapiga, Meggan, Rosenthal, Kim, Imamichi, Tomozumi, Chen, Yan, Chang, Chew-Shu, Chowdhury, Partha S, Naiman, Brian, Wang, Yue, Yang, De, Humbles, Alison A, Herbst, Ronald, Sims, Gary P
      PLoS ONE, 2015-02-23;10(2):e0115828.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    62. Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells.
      Authors: Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M
      Invest Ophthalmol Vis Sci, 2015-01-20;56(2):1051-62.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    63. Chemokine-coupled beta2 integrin-induced macrophage Rac2-Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis.
      Authors: Morrison A, Yarovinsky T, Young B, Moraes F, Ross T, Ceneri N, Zhang J, Zhuang Z, Sinusas A, Pardi R, Schwartz M, Simons M, Bender J
      J Exp Med, 2014-09-01;211(10):1957-68.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    64. Technical advance: live-imaging analysis of human dendritic cell migrating behavior under the influence of immune-stimulating reagents in an organotypic model of lung.
      Authors: Nguyen Hoang A, Chen P, Bjornfot S, Hogstrand K, Lock J, Grandien A, Coles M, Svensson M
      J Leukoc Biol, 2014-06-04;96(3):481-9.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    65. Characterization of platelet-monocyte complexes in HIV-1-infected individuals: possible role in HIV-associated neuroinflammation.
      Authors: Singh, Meera V, Davidson, Donna C, Jackson, Joseph W, Singh, Vir B, Silva, Jharon, Ramirez, Servio H, Maggirwar, Sanjay B
      J Immunol, 2014-04-11;192(10):4674-84.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    66. Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.
      Authors: Italiani, Paola, Mazza, Emilia M, Lucchesi, Davide, Cifola, Ingrid, Gemelli, Claudia, Grande, Alexis, Battaglia, Cristina, Bicciato, Silvio, Boraschi, Diana
      PLoS ONE, 2014-02-03;9(2):e87680.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    67. CD14(hi)HLA-DR(dim) macrophages, with a resemblance to classical blood monocytes, dominate inflamed mucosa in Crohn's disease.
      Authors: Thiesen S, Janciauskiene S, Uronen-Hansson H, Agace W, Hogerkorp C, Spee P, Hakansson K, Grip O
      J Leukoc Biol, 2013-11-08;95(3):531-41.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    68. Novel role for p21-activated kinase 2 in thrombin-induced monocyte migration.
      Authors: Gadepalli R, Kotla S, Heckle M, Verma S, Singh N, Rao G
      J Biol Chem, 2013-09-11;288(43):30815-31.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    69. Immunogenic calreticulin exposure occurs through a phylogenetically conserved stress pathway involving the chemokine CXCL8.
      Authors: Sukkurwala A, Martins I, Wang Y, Schlemmer F, Ruckenstuhl C, Durchschlag M, Michaud M, Senovilla L, Sistigu A, Ma Y, Vacchelli E, Sulpice E, Gidrol X, Zitvogel L, Madeo F, Galluzzi L, Kepp O, Kroemer G
      Cell Death Differ, 2013-06-21;21(1):59-68.
    70. Nuclear factor of activated T cells c1 mediates p21-activated kinase 1 activation in the modulation of chemokine-induced human aortic smooth muscle cell F-actin stress fiber formation, migration, and proliferation and injury-induced vascular wall remodeling.
      Authors: Kundumani-Sridharan, Venkates, Singh, Nikhlesh, Kumar, Sanjay, Gadepalli, Ravisekh, Rao, Gadipart
      J Biol Chem, 2013-06-04;288(30):22150-62.
      Species: Human
      Sample Types: Whole Cells
      Applications: Cell Culture
    71. The novel role of IL-7 ligation to IL-7 receptor in myeloid cells of rheumatoid arthritis and collagen-induced arthritis.
      Authors: Chen Z, Kim S, Chamberlain N, Pickens S, Volin M, Volkov S, Arami S, Christman J, Prabhakar B, Swedler W, Mehta A, Sweiss N, Shahrara S
      J Immunol, 2013-04-19;190(10):5256-66.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    72. Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
      Authors: van Gils, Janine M, Ramkhelawon, Bhama, Fernandes, Luciana, Stewart, Merran C, Guo, Liang, Seibert, Tara, Menezes, Gustavo, Cara, Denise C, Chow, Camille, Kinane, T Bernar, Fisher, Edward A, Balcells, Mercedes, Alvarez-Leite, Jacqueli, Lacy-Hulbert, Adam, Moore, Kathryn
      Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):911-9.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    73. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
      Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
      Nature, 2012-07-26;487(7408):505-9.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    74. Xanthine oxidoreductase promotes the inflammatory state of mononuclear phagocytes through effects on chemokine expression, peroxisome proliferator-activated receptor-gamma sumoylation, and HIF-1alpha.
      Authors: Gibbings S, Elkins ND, Fitzgerald H, Tiao J, Weyman ME, Shibao G, Fini MA, Wright RM
      J. Biol. Chem., 2010-11-08;286(2):961-75.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    75. Enhanced tumor trafficking of GD2 chimeric antigen receptor T cells by expression of the chemokine receptor CCR2b.
      Authors: Craddock J, Lu A, Bear A, Pule M, Brenner M, Rooney C, Foster A
      J Immunother, 2010-10-01;33(8):780-8.
      Species: Human
      Sample Types: Whole Cells
      Applications: CAR-T (Bioassay)
    76. A multiplex immunoassay for human adipokine profiling.
      Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
      Clin. Chem., 2010-06-08;56(0):1320.
      Applications: ELISA (Standard)
    77. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
      Authors: Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
      J. Immunol., 2009-12-21;184(3):1642-52.
      Species: Human
      Sample Types: Carbohydrates
      Applications: Bioassay
    78. CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B.
      Authors: Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M
      Immunology, 2009-12-02;129(4):536-46.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    79. IL-17 induces monocyte migration in rheumatoid arthritis.
      Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
      J. Immunol., 2009-03-15;182(6):3884-91.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    80. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
      Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
      J. Immunol., 2008-12-01;181(11):8053-67.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    81. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
      Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
      J. Leukoc. Biol., 2008-06-30;84(4):1109-19.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    82. Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
      Authors: Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
      Blood, 2008-05-14;112(3):493-503.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    83. The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes.
      Authors: Burt D, Salvidio G, Tarabra E, Barutta F, Pinach S, Dentelli P, Camussi G, Perin PC, Gruden G
      Am. J. Pathol., 2007-11-30;171(6):1789-99.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    84. TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC.
      Authors: Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T
      Arterioscler. Thromb. Vasc. Biol., 2007-11-08;28(1):135-41.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    85. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
      Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
      Clin. Chem., 2007-11-01;53(11):2010-2.
      Applications: ELISA (Standard)
    86. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
      Authors: Pfleger C, Schloot N, ter Veld F
      J. Immunol. Methods, 2007-10-22;329(1):214-8.
      Applications: ELISA (Standard)
    87. Ultrasensitive flow-based immunoassays using single-molecule counting.
      Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
      Clin. Chem., 2007-09-21;53(11):1990-5.
      Applications: ELISA (Standard)
    88. Tumor-derived chemokine MCP-1/CCL2 is sufficient for mediating tumor tropism of adoptively transferred T cells.
      Authors: Brown CE, Vishwanath RP, Aguilar B, Starr R, Najbauer J, Aboody KS, Jensen MC
      J. Immunol., 2007-09-01;179(5):3332-41.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    89. Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
      Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
      Biochem. Biophys. Res. Commun., 2007-05-02;358(2):528-33.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    90. CD16+ monocyte-derived macrophages activate resting T cells for HIV infection by producing CCR3 and CCR4 ligands.
      Authors: Ancuta P, Autissier P, Wurcel A, Zaman T, Stone D, Gabuzda D
      J. Immunol., 2006-05-15;176(10):5760-71.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    91. Novel antiviral activity of chemokines.
      Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
      Virology, 2006-04-17;350(2):484-92.
      Species: Virus
      Sample Types: Virus, Whole Cells
      Applications: Binding Assay, Bioassay
    92. Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation.
      Authors: Nguyen AN, Stebbins EG, Henson M, O&amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS
      Exp. Cell Res., 2006-04-04;312(10):1909-23.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    93. HIV-1 tat protein induces a migratory phenotype in human fetal microglia by a CCL2 (MCP-1)-dependent mechanism: possible role in NeuroAIDS.
      Authors: Eugenin EA, Dyer G, Calderon TM, Berman JW
      Glia, 2005-03-01;49(4):501-10.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    94. trans fatty acids and systemic inflammation in heart failure.
      Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
      Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
      Applications: ELISA (Standard)
    95. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
      Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
      J. Immunol., 2004-12-01;173(11):6592-602.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    96. Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2.
      Authors: Traves SL, Smith SJ, Barnes PJ, Donnelly LE
      J. Leukoc. Biol., 2004-05-20;76(2):441-50.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    97. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
      Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
      Blood, 2004-03-25;104(2):314-20.
      Applications: ELISA (Standard)
    98. Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain.
      Authors: Banisadr G, Queraud-Lesaux F, Boutterin MC, Pelaprat D, Zalc B, Rostene W, Haour F, Parsadaniantz SM
      J. Neurochem., 2002-04-01;81(2):257-69.
      Species: Rat
      Sample Types: Whole Tissue
      Applications: Binding Assay

    FAQs

    1. Should I use Recombinant Human CCL2/MCP-1 Protein formulated with carrier BSA (Catalog # 279-MC) or the Recombinant Human CCL2/MCP-1 Protein (Catalog # 279-MC/CF) that is carrier-free (no BSA) for my in vivo experiments?

      • For in vivo aplications, we would recommend using the carrier-free Recombinant Human CCL2/MCP-1 Protein (Catalog # 279-MC/CF). The injection of a carrier protein such as BSA can cause side effects and sometimes death to the experimental animal. For more information regarding use of the protein for in vivo applications, we would recommend consulting the Citations tab on the product-specific page.

    View all Proteins and Enzyme FAQs

    Reviews for Recombinant Human CCL2/MCP-1 Protein

    Average Rating: 4.6 (Based on 10 Reviews)

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    Recombinant Human CCL2/MCP-1 Protein
    By Anonymous on 11/12/2024
    Application: Media additive for protein or antibody production
    Media additive for protein or antibody production Recombinant Human CCL2/MCP-1 Protein 279-MC
    Recombinant Human CCL2/MCP-1 Protein
    By Anonymous on 05/14/2022
    Application: Migration
    Other Recombinant Human CCL2/MCP-1 Protein 279-MC
    Recombinant Human CCL2/MCP-1 Protein
    By Anonymous on 05/14/2022
    Application: Migration
    Other Recombinant Human CCL2/MCP-1 Protein 279-MC
    Recombinant Human CCL2/MCP-1 Protein
    By vishal patel on 03/03/2022
    Application: In vivo study
    Reason for Rating: stable kit

    DSS treated mice serum samples

    In vivo study Recombinant Human CCL2/MCP-1 Protein 279-MC
    Recombinant Human CCL2/MCP-1 Protein
    By Anonymous on 10/21/2021
    Application: Cell migration/motility
    Recombinant Human CCL2/MCP-1 Protein
    By Anonymous on 06/09/2021
    Application: Cell migration/motility
    Cell migration/motility Recombinant Human CCL2/MCP-1 Protein 279-MC
    Recombinant Human CCL2/MCP-1 Protein
    By jayeeta giri on 07/08/2019
    Application: In vitro bioactivity in cell culture
    Recombinant Human CCL2/MCP-1 Protein
    By Zoia Levashova on 11/26/2018
    Application: Immunoassay Standard

    The recombinant human CCL2 protein was used as the calibration reagent in MSD assay with biotinylated MAB279 (capture) and Sulfo-Tag labeled AF-279-NA (detection) antibodies.

    Immunoassay Standard Recombinant Human CCL2/MCP-1 Protein 279-MC
    Recombinant Human CCL2/MCP-1 Protein
    By Balaji Mahender on 02/05/2018
    Application: Immunoassay Standard
    Recombinant Human CCL2/MCP-1 Protein
    By Anonymous on 06/13/2017
    Application: Cell migration/motility
    Reason for Rating: I have been using this protein for cell migration and cell signaling assays and it works very well.

    MCP1-induced smooth muscle cells migration using wound closure assay. I use at 50 ng/ml concentration in serum-free media.

    Cell migration/motility Recombinant Human CCL2/MCP-1 Protein 279-MC