Recombinant Human CCL28 Protein Summary
Product Specifications
Ile23-Tyr127
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
717-VC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
717-VC/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant Human CCL28 (Catalog # 717-VC) chemoattracts the BaF3 mouse pro-B cells transfected with mouse CCR10. The ED50 for this effect is 0.4‑2.0 μg/mL.
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1 μg/lane of Recombinant Human CCL28 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Reconstitution Calculator
Background: CCL28
Human CCL28 (CC chemokine ligand 28) is a novel CC chemokine identified by TBLASTN searches of the Human Genome Systems (HGS) and Genbank dbEst database using a human chemokine consensus sequence. Human CCL28 cDNA encodes a 127 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 105 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Human and mouse CCL28 RNA expression was found to be highest in normal and pathologic colon with the protein being expressed by epithelial cells. Human CCL28 RNA was also present in normal and asthmatic lung tissues. The receptor for CCL28 has been identified as the CCR10 (GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.
Citations for Recombinant Human CCL28 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals
Authors: M Nayrac, M Requena, C Loiseau, M Cazabat, B Suc, N Carrere, K Barange, L Alric, G Martin-Blo, J Izopet, P Delobel
Mucosal Immunol, 2020-04-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CCL27/CCL28-CCR10 CHEMOKINE SIGNALING MEDIATES MIGRATION OF LYMPHATIC ENDOTHELIAL CELLS
Authors: T Karnezis, R Farnsworth, NC Harris, SP Williams, C Caesar, DJ Byrne, P Herle, ML Macheda, R Shayan, YF Zhang, S Yazar, SJ Takouridis, C Gerard, S Fox, MG Achen, SA Stacker
Cancer Res., 2019-02-01;0(0):.
Species: Human, Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells
Authors: Guichun Huang
Sci Rep, 2016-06-02;6(0):27152.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Chemokine-enhanced chemotaxis of lymphangioleiomyomatosis cells with mutations in the tumor suppressor TSC2 gene.
Authors: Pacheco-Rodriguez G, Kumaki F, Steagall WK, Zhang Y, Ikeda Y, Lin JP, Billings EM, Moss J
J. Immunol., 2009-02-01;182(3):1270-7.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Novel antiviral activity of chemokines.
Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
Virology, 2006-04-17;350(2):484-92.
Species: Virus
Sample Types: Virus, Whole Cells
Applications: Binding Assay, Bioassay -
CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity.
Authors: Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O
J. Immunol., 2003-02-01;170(3):1452-61.
Species: Human
Sample Types: N/A, Whole Cells
Applications: Bioassay, ELISA (Standard), Western Blot -
Expression of functional CCR and CXCR chemokine receptors in podocytes.
Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
J. Immunol., 2002-06-15;168(12):6244-52.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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