Recombinant Human CD19 Fc Chimera Protein, CF Summary
Why choose R&D Systems CD19 Protein?
- Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 90%.
- Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
- Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
- Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.
NEW! Fluorescent labeled CD19 designed for validating expression of a CD19 chimeric antigen receptor (CAR) Fluorescent CD19 Proteins
Product Specifications
| Human CD19 (Glu21-Lys291) Accession # P15391 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9269-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human CD19 Fc Chimera 9269-CD is immobilized at 2 µg/mL to 100 µL/well, recombinant biotinylated human CD81 Fc Chimera binds with an ED50 of 4-20 μg/mL.
Reconstitution Calculator
Background: CD19
CD19 protein is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses (1, 2). CD19 is expressed throughout B cell development from pre-B cells through mature B cells, and it is commonly used as a B cell lineage marker (1). It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation (4, 5). The CD19 protein associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu13, (6-9). These associations enable CD19 to amplify B cell signaling (7, 9-12) and reduce the threshold for antigen stimulation through the BCR (13). CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production (2). CD19 has emerged as promising therapeutic target for hematologic cancers and solid tumors. Immunotherapy using a chimeric antigen receptor (CAR) targeting CD19 has proven effective in many different cancers. The first CD19 CAR T cell therapies have been granted FDA approval for the treatment of B cell malignancies with several more in clinical trials.
Mature human CD19 protein consists of a 272 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 22 aa transmembrane segment, and a 243 aa cytoplasmic domain (3). Within the ECD, human CD19 shares 57% amino acid sequence identity with mouse and rat CD19.
- Wang, K. et al. (2012) Exp. Hematol. Oncol. 1:36.
- Tedder, T.F. et al. (1997) Immunity 6:107.
- Tedder, T.F. and C.M. Isaacs (1989) J. Immunol. 143:712.
- Rickert, R.C. et al. (1995) Nature 376:352.
- Engel, P. et al. (1995) Immunity 3:39.
- Vences-Catalan, F. et al. (2012) J. Immunol. 137:48.
- Deaglio, S. et al. (2007) Blood 109:5390.
- Bradbury, L. et al. (1992) J. Immunol. 149:2841.
- Krop, I. et al. (1996) J. Immunol. 157:48.
- O'Rourke, L.M. et al. (1998) Immunity 8:635.
- Buhl, A.M. et al. (1997) J. Exp. Med. 186:1897.
- Sato, S. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11558.
- Carter, R.H. and D.T. Fearon (1992) Science 256:105.
Citations for Recombinant Human CD19 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Murine CAR19 Tregs suppress acute graft-versus-host disease and maintain graft-versus-tumor responses
Authors: S Bolivar-Wa, ML Loschi, S Jin, G Thangavelu, JH Larson, CS McDonald-H, EA Aguilar, A Saha, BH Koehn, M Hefazi, MJ Osborn, MC Jensen, JE Wagner, CA Pennell, BR Blazar
JCI Insight, 2022-09-08;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A rational approach to assess off-target reactivity of a dual-signal integrator for T cell therapy
Authors: X Wang, LM Wong, ME McElvain, S Martire, WH Lee, CZ Li, FA Fisher, RL Maheshwari, ML Wu, MC Imun, R Murad, DT Warshaviak, J Yin, A Kamb, H Xu
Toxicology and Applied Pharmacology, 2022-01-25;0(0):115894.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
HLA-independent T cell receptors for targeting tumors with low antigen density
Authors: J Mansilla-S, J Eyquem, S Haubner, M Hamieh, J Feucht, N Paillon, AE Zucchetti, Z Li, M Sjöstrand, PL Lindenberg, M Saetersmoe, A Dobrin, M Maurin, A Iyer, A Garcia Ang, MM Miele, Z Zhao, T Giavridis, SJC van der St, F Tamzalit, I Rivière, M Huse, RC Hendrickso, C Hivroz, M Sadelain
Nature Medicine, 2022-01-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Liposome-based nanocarriers loaded with anthrax lethal factor and armed with anti-CD19 VHH for effectively inhibiting MAPK pathway in B cells
Authors: SR Banihashem, F Rahbarizad, A Zavaran Ho, D Ahmadvand, S Khoshtinat
International immunopharmacology, 2021-09-06;100(0):107927.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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