Recombinant Human CD3 gamma Fc Chimera Protein, CF Summary
Product Specifications
| Human CD3 gamma (Gln23-Ser116) Accession # P09693.1 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10550-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human CD3 gamma Fc Chimera (Catalog # 10550-CD) is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3E Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-4 μg/mL.
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2 μg/lane of Recombinant Human CD3 gamma Fc Chimera Protein (Catalog # 10550-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42-53 kDa and 80-110 kDa, respectively.
Reconstitution Calculator
Background: CD3 gamma
T-cell surface glycoprotein CD3 gamma chain, also known as CD3G, is a 20 kDa single-pass type I membrane protein that is part of the TCR-CD3 complex. CD3 gamma forms a heterodimer with CD3 epsilon which associates with TCR beta, while CD3δ/CD3 epsilon heterodimer associates with TCR alpha. CD3 gamma /CD3 epsilon /TCR beta joins CD3δ/CD3 epsilon /TCR alpha and recruits a CD3 zeta homodimer to form the complete TCR-CD3 complex (1). CD3 chains contain one cytoplasmic ITAM domain which mediates intracellular signal transduction (2). The extracellular domain of CD3 gamma contains one disulfide bond and 2 N-linked glycosylation sites. The ECD of human CD3 gamma shares 60% and 62% sequence identity with mouse and rat CD3 gamma, respectively. The biologic roles of each CD3 chain and their relative contribution to the signals transduced through the TCR/CD3 complex and to downstream activation events are still uncertain, whether they may be specialized or redundant (3). Deficiencies in CD3 gamma can cause impairments in immune function, particularly in Treg cells which may contribute to autoimmune disorders (4).
- Kuhns, M. et al. (2006). Immunity 24:133.
- Hayes, S.M. et al. (2003). Immunol. Rev. 191:28.
- Pacheco-Castro, A. et al. (1998) J. Immunol. 161:3152.
- Rowe, J. et al. (2018). Blood. 131:2335.
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