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Recombinant Human CD35 Protein, CF

Catalog #: 5748-CD
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5748-CD-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (6)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD35 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When rhCD35 is coated at 5 μg/mL, complement component C3b binds with an apparent KD <10 nM.
Source
Mouse myeloma cell line, NS0-derived human CD35 protein
Gln42-Asp1971, with a C-terminal 10-His tag and a substitution of His1208Arg
Accession #
CAA68755
N-terminal Sequence
Analysis
No results obtained: Gln42 predicted
Predicted Molecular Mass
213 kDa
SDS-PAGE
200-220 kDa, reducing conditions

Product Datasheets

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5748-CD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5748-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD35

CD35, also known as complement receptor 1 (CR1), is an N-glycosylated member of the RCA (regulators of complement activation) family of proteins. The complement cascade plays an important role in the innate immune system through the recognition and clearance of immune complexes and foreign particles. There are four major allotypes of human CD35 which range from approximately 220 - 300 kDa (1, 2). Mature human CD35 contains a 1930 amino acid (aa) extracellular domain (ECD) with 30 tandem SCR/SUSHI repeats, a 25 aa transmembrane segment, and a 43 aa cytoplasmic tail (3). A mouse ortholog of human CD35 has not been described, although alternate splicing of mouse CD21/CR2 generates a protein with homology to some SCR repeats of human CD35 (4). A soluble form of the CD35 ECD circulates in the serum (5). Cell surface CD35 is widely expressed on hematopoietic cells (1). It cooperates with both CD21 and CR3/MAC-1 in the binding and internalization of particles and immune complexes that are opsonized with complement components C3b, C3i, and C4b (1, 6, 7). It also binds and internalizes particles opsonized with complement factor C1q or mannose binding lectin (MBL) (8, 9). CD35 protects the cell from complement-mediated lysis by serving as a cofactor for Factor I and inhibiting the C3 and C5 convertases (10). Erythrocyte-expressed CD35 mediates the hepatic clearance of complement-opsonized immune complexes (11). On lymphocytes, CD35 inhibits the activation-induced proliferation of T cells and B cells (12, 13). Mice deficient in both CD21 and CD35 exhibit normal B cell development but severely compromised germinal center development, antibody production, and establishment of protective microbial immunity (14). In mice, CD21/CD35 must additionally be present on follicular dendritic cells to mount effective humoral responses and establishment of B cell memory (15).

References
  1. Roozendaal, R. and M.C. Carroll (2007) Immunol. Rev. 219:157.
  2. Wong, W.W. et al. (1989) J. Exp. Med. 169:847.
  3. Klickstein, L.B. et al. (1987) J. Exp. Med. 165:1095.
  4. Kurtz, C.B. et al. (1990) J. Immunol. 144:3581.
  5. Yoon, S.H. and D.T. Fearon (1985) J. Immunol. 134:3332.
  6. Tuveson, D.A. et al. (1991) J. Exp. Med. 173:1083.
  7. Sutterwala, F.S. et al. (1996) J. Leukoc. Biol. 59:883.
  8. Klickstein, L.B. et al. (1997) Immunity 7:345.
  9. Ghiran, I. et al. (2000) J. Exp. Med. 192:1797.
  10. Iida, K. and V. Nussenzweig (1981) J. Exp. Med. 153:1138.
  11. Cornacoff, J.B. et al. (1983) J. Clin. Invest. 71:236.
  12. Wagner, C. et al. (2006) Mol. Immunol. 43:643.
  13. Jozsi, M. et al. (2002) J. Immunol. 168:2782.
  14. Haas, K.M. et al. (2002) Immunity 17:713.
  15. Rossbacher, J. et al. (2006) Eur. J. Immunol. 36:2384.
Entrez Gene IDs
1378 (Human)
Alternate Names
C3BR; CD35; complement component (3b/4b) receptor 1 (Knops blood group); CR1; KN

Citations for Recombinant Human CD35 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. An Immunoreceptor-Targeting Strategy with Minimalistic C3b Peptide Fusion Enhances SARS-CoV-2 RBD mRNA Vaccine Immunogenicity
    Authors: Chiu, CT;Tsai, HH;Chen, JY;Hu, CJ;Chen, HW;
    International journal of nanomedicine
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Complement receptor 1 is the human erythrocyte receptor for Plasmodium vivax erythrocyte binding protein
    Authors: Lee, SK;Crosnier, C;Valenzuela-Leon, PC;Dizon, BLP;Atkinson, JP;Mu, J;Wright, GJ;Calvo, E;Gunalan, K;Miller, LH;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: N/A, Human
    Sample Types: Recombinant Protein, Whole Cells
    Applications: ELISA Capture, Bioassay
  3. Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes
    Authors: SK Prajapati, C Borlon, E Rovira-Val, J Gruszczyk, S Menant, WH Tham, JH Kattenberg, E Villasis, K De Meulena, D Gamboa, J Vinetz, R Fujita, XN Xuan, M Urbano Fer, CH Niño, MA Patarroyo, G Spanakos, L Kestens, JVD Abbeele, A Rosanas-Ur
    Sci Rep, 2019-06-20;9(1):8943.
    Species: P. vivax
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Tissue plasminogen activator mediates deleterious complement cascade activation in stroke
    Authors: XJ Zhao, TM Larkin, MA Lauver, S Ahmad, AF Ducruet
    PLoS ONE, 2017-07-10;12(7):e0180822.
    Applications: Bioassay
  5. Three pentraxins C-reactive protein, serum amyloid p component and pentraxin 3 mediate complement activation using Collectin CL-P1
    Authors: Nobutaka Wakamiya
    Biochim. Biophys. Acta, 2016-11-15;0(0):.
    Applications: Bioassay
  6. Complement factor H-related hybrid protein deregulates complement in dense deposit disease.
    Authors: Chen, Qian, Wiesener, Michael, Eberhardt, Hannes U, Hartmann, Andrea, Uzonyi, Barbara, Kirschfink, Michael, Amann, Kerstin, Buettner, Maike, Goodship, Tim, Hugo, Christia, Skerka, Christin, Zipfel, Peter F
    J Clin Invest, 2013-12-16;124(1):145-55.
    Species: Human
    Sample Types: Serum
    Applications: Bioassay

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