Recombinant Human CD36/SR-B3 Fc Chimera Protein, CF

Catalog #: 1955-CD
11 Citations Datasheet / COA / SDS

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1955-CD-050

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Citations (11)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD36/SR-B3 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD36/Fc Chimera is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human TSP-2/His that producecs 50% of the optimal binding response is fround to be 15-120 ng/mL.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CD36/SR-B3 protein

Human CD36 (Gly30-Asn439) Accession # P16671	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

P16671

N-terminal Sequence
Analysis

Gly30

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

73 kDa (monomer)

SDS-PAGE

53-64 kDa & 93 kDa, under reducing conditions.

Product Datasheets

1955-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1955-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CD36/SR-B3

CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta -amyloid fibrils (fA beta ), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.

References

Febbraio, M. et al. (2001) J. Clin. Invest. 108:785.
Khoury, J. et al. (2003) J. Exp. Med. 197:1657.
Husemann, J. et al. (2002) Glia 40:195.
Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63.
Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.

Long Name

Scavenger Receptor Class B, Member 3

Entrez Gene IDs

948 (Human); 12491 (Mouse)

Alternate Names

BDPLT10; CD_antigen: CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 antigen; CD36 molecule (thrombospondin receptor); CD36; CHDS7; cluster determinant 36; Collagen R; FAT; Fatty acid translocase; Glycoprotein IIIb; GP3B; GP4; GPIIIb; GPIV; PAS IV; PAS-4 protein; PAS-4; PASIV; Platelet collagen receptor; platelet glycoprotein 4; SCARB3; scavenger receptor class B, member 3; SRB3; SR-B3; Thrombospondin R; Thrombospondin receptor

Related Research Areas

Citations for Recombinant Human CD36/SR-B3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Developing a multivariate prediction model of antibody features associated with protection of malaria-infected pregnant women from placental malaria
Authors: EH Aitken, T Damelang, A Ortega-Paj, A Alemu, W Hasang, S Dini, HW Unger, M Ome-Kaius, MA Nielsen, A Salanti, J Smith, S Kent, PM Hogarth, BD Wines, JA Simpson, A Chung, SJ Rogerson
Elife, 2021-06-29;10(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Hydrophobicity drives receptor-mediated uptake of heat-processed proteins by THP-1 macrophages and dendritic cells, but not cytokine responses
Authors: Y Deng, C Govers, M Teodorowic, I Liobyte, I De Simone, K Hettinga, HJ Wichers
PLoS ONE, 2020-08-14;15(8):e0236212.
Species: Bovine
Sample Types: Protein
Applications: Bioassay
Preclinical immunogenicity and safety of the cGMP-grade placental malaria vaccine PRIMVAC
Authors: A Chêne, S Gangnard, A Guadall, H Ginisty, O Leroy, N Havelange, NK Viebig, B Gamain
EBioMedicine, 2019-03-15;0(0):.
Species: Parasite - Plasmodium falciparum
Sample Types: Whole Cells
Applications: Bioassay
TREM2 Is a Receptor for ?-Amyloid that Mediates Microglial Function
Authors: Y Zhao, X Wu, X Li, LL Jiang, X Gui, Y Liu, Y Sun, B Zhu, JC Piña-Cresp, M Zhang, N Zhang, X Chen, G Bu, Z An, TY Huang, H Xu
Neuron, 2018-03-07;97(5):1023-1031.e7.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Beninese children with cerebral malaria do not develop humoral immunity against the IT4-VAR19-DC8 PfEMP1 variant linked to EPCR and brain endothelial binding.
Authors: Nunes-Silva S, Dechavanne S, Moussiliou A, Pstrag N, Semblat J, Gangnard S, Tuikue-Ndam N, Deloron P, Chene A, Gamain B
Malar J, 2015-12-08;14(1):493.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Dynamics in the cytoadherence phenotypes of Plasmodium falciparum infected erythrocytes isolated during pregnancy.
Authors: Doritchamou J, Sossou-tchatcha S, Cottrell G, Moussiliou A, Hounton Houngbeme C, Massougbodji A, Deloron P, Ndam N
PLoS ONE, 2014-06-06;9(6):e98577.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
A nasal epithelial receptor for Staphylococcus aureus WTA governs adhesion to epithelial cells and modulates nasal colonization.
Authors: Baur S, Rautenberg M, Faulstich M, Grau T, Severin Y, Unger C, Hoffmann W, Rudel T, Autenrieth I, Weidenmaier C
PLoS Pathog, 2014-05-01;10(5):e1004089.
Applications: Bioassay
The C-terminal segment of the cysteine-rich interdomain of Plasmodium falciparum erythrocyte membrane protein 1 determines CD36 binding and elicits antibodies that inhibit adhesion of parasite-infected erythrocytes.
Authors: Mo M, Lee HC, Kotaka M, Niang M, Gao X, Iyer JK, Lescar J, Preiser P
Infect. Immun., 2008-02-25;76(5):1837-47.
Species: Human
Sample Types:
Applications: ELISA Developmet
Investigation of binding proteins for anti-platelet agent K-134 by Drug-Western method.
Authors: Ikenoya M, Doi T, Miura T, Sawanobori K, Nishio M, Hidaka H
Biochem. Biophys. Res. Commun., 2006-12-29;353(4):1111-4.
Species: Human
Sample Types: Complex Sample Type
Applications: Bioassay
The frequency of BDCA3-positive dendritic cells is increased in the peripheral circulation of Kenyan children with severe malaria.
Authors: Urban BC, Cordery D, Shafi MJ, Bull PC, Newbold CI, Williams TN, Marsh K
Infect. Immun., 2006-09-25;74(12):6700-6.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Whole-body imaging of sequestration of Plasmodium falciparum in the rat.
Authors: Pettersson F, Vogt AM, Jonsson C, Mok BW, Shamaei-Tousi A, Bergstrom S, Chen Q, Wahlgren M
Infect. Immun., 2005-11-01;73(11):7736-46.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay