Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed)
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Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed) Summary
Product Specifications
| Hemagglutinin tag YPYDVPDYA |
GCN4-IZ |
GGGSGGGSGGGS | Human CD40 Ligand (Met113-Leu261) Accession # P29965.1 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6420-CL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
6420-CL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and EDTA. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CD40 Ligand/TNFSF5
CD40 Ligand, also known as TNFSF, CD154, TRAP, and gp39, is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily (1-3). Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region (4, 5). The extracellular domain of human CD40 Ligand shares 74% and 76% aa sequence identity with mouse and rat CD40 Ligand, respectively. Similar to other TNF superfamily members, CD40 Ligand forms a bioactive homotrimer, both as membrane bound and soluble forms (6-9). The 18 kDa soluble form (aa 113-261) arises from proteolytic processing. Mutation and alternative splicing generate additional forms of CD40 Ligand that are often truncated or non-trimerizable (8). CD40 Ligand is expressed on platelets, as well as on activated T cells and B cells, basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds to CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial, and epithelial cells (10). The interaction of CD40 Ligand with CD40 initiates signaling in both CD40 and CD40 Ligand expressing cells (11). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (12, 13). CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS (14, 15). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (16, 17).
- Zhang, G. (2004) Curr. Opin. Struct. Biol. 14:154.
- Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
- Quezada, S.A. et al. (2004) Annu. Rev. Immunol. 22:307.
- Graf, D. et al. (1992) Eur. J. Immunol. 22:3191.
- Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
- Khandekar, S.S. et al. (2001) Protein Expr. Purif. 23:301.
- Pietravalle, F. et al. (1996) J. Biol. Chem. 271:5965.
- Garber, E. et al. (1999) J. Biol. Chem. 274:33545.
- Vakkalanka, R.K. et al. (1999) Arthritis Rheum. 42:871.
- van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
- Eissner, G. et al. (2004) Cytokine Growth Factor. Rev. 15:353.
- Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
- Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
- Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
- Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
- Pamukcu, B. et al. (2011) Ann. Med. 43:331.
- Hassan, G.S. et al. (2012) Immunobiology 217:521.
Citations for Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed)
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Rapid discovery of monoclonal antibodies by microfluidics-enabled FACS of single pathogen-specific antibody-secreting cells
Authors: Fischer, K;Lulla, A;So, TY;Pereyra-Gerber, P;Raybould, MIJ;Kohler, TN;Yam-Puc, JC;Kaminski, TS;Hughes, R;Pyeatt, GL;Leiss-Maier, F;Brear, P;Matheson, NJ;Deane, CM;Hyvönen, M;Thaventhiran, JED;Hollfelder, F;
Nature biotechnology
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy
Authors: Abu El-Asrar, AM;Nawaz, MI;Ahmad, A;Dillemans, L;Siddiquei, M;Allegaert, E;Gikandi, PW;De Hertogh, G;Opdenakker, G;Struyf, S;
International journal of molecular sciences
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity
Authors: R Leylek, M Alcántara-, JM Granja, M Chavez, K Perez, OR Diaz, R Li, AT Satpathy, HY Chang, J Idoyaga
Cell Rep, 2020-09-22;32(12):108180.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cholesterol metabolism drives regulatory B cell IL-10 through provision of geranylgeranyl pyrophosphate
Authors: JA Bibby, HA Purvis, T Hayday, A Chandra, K Okkenhaug, S Rosenzweig, I Aksentijev, M Wood, HJ Lachmann, C Kemper, AP Cope, E Perucha
Nat Commun, 2020-07-08;11(1):3412.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Aspirin Reduces the Potentiating Effect of CD40L on Platelet Aggregation via Inhibition of Myosin Light Chain
Authors: K Kojok, M Mohsen, AEH El Kadiry, W Mourad, Y Merhi
J Am Heart Assoc, 2020-02-03;9(3):e013396.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
CD24hiCD38hi and CD24hiCD27+ Human Regulatory B Cells Display Common and Distinct Functional Characteristics
Authors: MM Hasan, L Thompson-S, G Klintmalm, AJ Demetris, J O'Leary, S Oh, H Joo
J. Immunol., 2019-09-11;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An essential role for the Zn2+ transporter ZIP7 in B cell development
Authors: C Anzilotti, DJ Swan, B Boisson, M Deobagkar-, C Oliveira, P Chabosseau, KR Engelhardt, X Xu, R Chen, L Alvarez, R Berlinguer, KR Bull, E Cawthorne, AP Cribbs, TL Crockford, TS Dang, A Fearn, EJ Fenech, SJ de Jong, BC Lagerholm, CS Ma, D Sims, B van den Be, Y Xu, AJ Cant, G Kleiner, TR Leahy, MT de la More, JM Puck, RS Shapiro, M van der Bu, JR Chapman, JC Christians, B Davies, JA McGrath, S Przyborski, M Santibanez, SG Tangye, A Werner, GA Rutter, S Padilla-Pa, JL Casanova, RJ Cornall, ME Conley, S Hambleton
Nat. Immunol., 2019-02-04;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Condition-dependent generation of aquaporin-4 antibodies from circulating B cells in neuromyelitis optica
Authors: R Wilson, M Makuch, AK Kienzler, J Varley, J Taylor, M Woodhall, J Palace, MI Leite, P Waters, SR Irani
Brain, 2018-04-01;141(4):1063-1074.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
MAbs, 2018-03-05;0(0):1-43.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
Distinct CD40L receptors mediate inflammasome activation and secretion of IL-1? and MCP-1 in cultured human retinal pigment epithelial cells
Authors: ZM Bian, MG Field, SG Elner, JM Kahlenberg, VM Elner
Exp. Eye Res., 2018-02-16;170(0):29-39.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation
Authors: J Dai, MA Luftig
J. Immunol., 2018-01-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Epstein-Barr Virus Induces Adhesion Receptor CD226 (DNAM-1) Expression during Primary B-Cell Transformation into Lymphoblastoid Cell Lines
Authors: L Grossman, C Chang, J Dai, PA Nikitin, DD Jima, SS Dave, MA Luftig
mSphere, 2017-11-29;2(6):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
EZH2 inhibition by tazemetostat results in altered dependency on B-cell activation signaling�in DLBCL
Authors: D Brach, D Johnston-B, A Drew, T Lingaraj, V Motwani, NM Warholic, I Feldman, C Plescia, JJ Smith, RA Copeland, H Keilhack, E Chan-Peneb, SK Knutson, SA Ribich, A Raimondi, MJ Thomenius
Mol. Cancer Ther., 2017-08-23;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CD40L-Dependent Pathway Is Active at Various Stages of Rheumatoid Arthritis Disease Progression
Authors: Y Guo, AM Walsh, U Fearon, MD Smith, MD Wechalekar, X Yin, S Cole, C Orr, T McGarry, M Canavan, S Kelly, TA Lin, X Liu, SM Proudman, DJ Veale, C Pitzalis, S Nagpal
J. Immunol., 2017-04-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Epstein-Barr virus ensures B cell survival by uniquely modulating apoptosis at early and late times after infection
Authors: AM Price, J Dai, Q Bazot, L Patel, PA Nikitin, R Djavadian, PS Winter, CA Salinas, AP Barry, KC Wood, EC Johannsen, A Letai, MJ Allday, MA Luftig
Elife, 2017-04-20;6(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TLR-mediated STAT3 and ERK activation controls IL-10 secretion by human B cells.
Authors: Liu B, Cao Y, Huizinga T, Hafler D, Toes R
Eur J Immunol, 2014-04-16;44(7):2121-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Mitogen-induced B-cell proliferation activates Chk2-dependent G1/S cell cycle arrest.
Authors: Nikitin, Pavel A, Price, Alexande, McFadden, Karyn, Yan, Christop, Luftig, Micah A
PLoS ONE, 2014-01-30;9(1):e87299.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
B cells as a therapeutic target for IFN-beta in relapsing-remitting multiple sclerosis.
Authors: Ramgolam VS, Sha Y, Marcus KL, Choudhary N, Troiani L, Chopra M, Markovic-Plese S
J. Immunol., 2011-03-02;186(0):4518.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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What is the purpose of the GCN4-IZ part of Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed) (Catalog # 6420-CL)?
CD40 Ligand naturally forms homotrimers, both as membrane bound and soluble forms. GCN4-IZ is a trimerization domain that promotes homotrimerization. We expect #6420-CL to include trimeric species; however, we do not have a structural specification for these products, relying instead on bioassay with a cross-linking antibody such as Anti-Hemagglutinin/HA Peptide antibody (Catalog # MAB060) to confirm consistency in bioactivity.
Reviews for Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed)
Average Rating: 4.5 (Based on 2 Reviews)
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Reason for Rating: Activates Nf-KB signaling in cultured CLL cells
