Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein, CF

Catalog #: AVI10380 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AVI10380-050

Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD40 Ligand/TNFSF5 Fc (HEK293-expressed, (Catalog # 6420-CL) mobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag (Catalog # AVI10380) that produces 50% optimal binding is 0.0400-0.320 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CD40/TNFRSF5 protein

Human CD40/TNFRSF5
(Glu21-Arg193)
Accession # P25942.1

IEGRMD

Human IgG₁
(Pro100-Lys330)

Avi-tag

Accession #

P25942.1

N-terminal Sequence
Analysis

Glu21

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Predicted Molecular Mass

48 kDa

SDS-PAGE

61-66 kDa, under reducing conditions

Product Datasheets

AVI10380

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10380

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed) Protein (Catalog # 6420-CL) is immobilized at 2 µg/mL, 100 µL/well, the concentration of Biotinylated Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag (Catalog # AVI10380) that produces 50% optimal binding is 0.0400-0.320 μg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag (Catalog # AVI10380) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-66 kDa and 120-135 kDa, respectively.

Reconstitution Calculator

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a 45-50 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily (1). Mature human CD40 consists of a 173 amino acid (aa) extracellular domain, a transmembrane domain, and a 62 aa cytoplasmic domain (2). The extracellular domain of human CD40 shares 58% and 56% aa sequence identity with mouse and rat CD40, respectively. An antagonistic soluble human CD40 splice variant contains an alternate sequence within the extracellular and transmembrane domains and lacks a cytoplasmic domain (3). CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (4, 5). Interaction of CD40 with its ligand, CD40 Ligand, leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40 Ligand expressing cells (6). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (7, 8). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (9, 10). Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (11, 12). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (13, 14).

References

Banchereau, J. et al. (1994) Annu. Rev. Immunol. 12:881.
Stamenkovic, I. et al. (1989) EMBO J. 8:1403.
Eshel, D. et al. (2008) Mol. Immunol. 46:250.
van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
Pamukcu, B. et al. (2011) Ann. Med. 43:331.
Hassan, G.S. et al. (2012) Immunobiology 217:521.

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)

Alternate Names

B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5