Recombinant Human CD40/TNFRSF5 Fc Chimera Protein, CF

Name: Recombinant Human CD40/TNFRSF5 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1493-CDB
Rating: 5 (1 reviews)

Catalog #: 1493-CDB
5 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1493-CDB-050

Recombinant Human CD40/TNFRSF5 Fc Chimera Protein Bioactivity

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All CD40/TNFRSF5 Proteins and Enzymes

Product Details

Citations (5)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD40/TNFRSF5 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD40/TNFRSF5 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) that produces 50% optimal binding is 4-24 ng/mL. Measured by its ability to inhibit rhCD40 Ligand-induced IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD40. The ED₅₀ for this effect is 0.08-0.8 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human CD40/TNFRSF5 protein

Human CD40 (Glu21-Arg193) Accession # P25942-1	DIEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

P25942-1

N-terminal Sequence
Analysis

Glu21

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

47 kDa

SDS-PAGE

58-66 kDa, reducing conditions

Product Datasheets

1493-CDB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1493-CDB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human CD40/TNFRSF5 Fc Chimera (Catalog # 1493-CDB) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) that produces 50% optimal binding is 4-24 ng/mL.

Reconstitution Calculator

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a 45-50 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily (1). Mature human CD40 consists of a 173 amino acid (aa) extracellular domain, a transmembrane domain, and a 62 aa cytoplasmic domain (2). The extracellular domain of human CD40 shares 58% and 56% aa sequence identity with mouse and rat CD40, respectively. An antagonistic soluble human CD40 splice variant contains an alternate sequence within the extracellular and transmembrane domains and lacks a cytoplasmic domain (3). CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (4, 5). Interaction of CD40 with its ligand, CD40 Ligand, leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40 Ligand expressing cells (6). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (7, 8). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (9, 10). Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (11, 12). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (13, 14).

References

Banchereau, J. et al. (1994) Annu. Rev. Immunol. 12:881.
Stamenkovic, I. et al. (1989) EMBO J. 8:1403.
Eshel, D. et al. (2008) Mol. Immunol. 46:250.
van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
Pamukcu, B. et al. (2011) Ann. Med. 43:331.
Hassan, G.S. et al. (2012) Immunobiology 217:521.

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)

Alternate Names

B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

Related Research Areas

Citations for Recombinant Human CD40/TNFRSF5 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Structural Diversity and Anticancer Activity of Marine-Derived Elastase Inhibitors: Key Features and Mechanisms Mediating the Antimetastatic Effects in Invasive Breast Cancer
Authors: FH Al-Awadhi, VJ Paul, H Luesch
Chembiochem, 2018-03-23;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Complex Interplay between Epitope Specificity and Isotype Dictates the Biological Activity of Anti-human CD40 Antibodies
Authors: X Yu, HTC Chan, CM Orr, O Dadas, SG Booth, LN Dahal, CA Penfold, L O'Brien, CI Mockridge, RR French, P Duriez, LR Douglas, AR Pearson, MS Cragg, I Tews, MJ Glennie, AL White
Cancer Cell, 2018-03-22;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR
Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells.
Authors: Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A
Nat Immunol, 2014-02-23;15(4):354-64.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
Blood, 2012-05-10;119(26):6268-77.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Decreased expression of tumor necrosis factor family receptors involved in humoral immune responses in preterm neonates.
Authors: Kaur K, Chowdhury S, Greenspan NS, Schreiber JR
Blood, 2007-07-18;110(8):2948-54.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay