Recombinant Human CD42b/GPIb alpha Protein, CF

Recombinant Human CD42b/GPIb alpha Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CD42b/GPIb alpha

Platelet glycoprotein Ib alpha chain (GPIb alpha ), also known as CD42b alpha, is a 145 kDa type I transmembrane protein that is a member of the leucine‑rich repeat (LRR) family of ligand binding proteins (1‑3). It is expressed by platelets as the ligand‑binding subunit of the platelet GPIb‑IX‑V complex (4). Human GPIb alpha contains a 16 amino acid (aa) signal sequence, a 489 aa extracellular domain (ECD), a 21‑aa transmembrane domain, and a 100 aa cytoplasmic region. The ECD contains 8 LRRs, with # 2, 3 and 4 having been demonstrated to regulate shear‑dependent adhesion to von Willebrand factor (vWF) (5, 6). The LRRs are followed by a thrombin‑binding anionic region that includes three sulfated tyrosines, a sialomucin domain with N‑ and O‑linked carbohydrates, and two cysteines near the membrane that allow dimerization with GP1b alpha beta (1‑6). Four human isoforms with 1 to 4 repeats of aa 398‑411 within the sialomucin domain of mature GPIb  alpha are known to exist but have unknown significance (7). The ECD of human GPIb alpha shares 48‑51% aa identity with mouse, rat, bovine and canine GPIb alpha. The metalloproteinase TACE/ADAM17 constitutively and inducibly cleaves GPIb alpha, between Gly 480 and Val 481. This releases a soluble form called glycocalicin that circulates at ~2 μg/mL (8, 9). GPIb alpha binding to ligands such as thrombin, kininogen, and coagulation factors XI and XII helps to initiate platelet activation and coordinate the coagulation cascade (1, 10‑12). Binding of GPIb alpha to vWF or thrombospondin in the plasma or matrix, vWF or P‑selectin on endothelial cells, or the integrin alpha M beta 2 (MAC‑1) on myeloid cells, controls response to vascular injury (1, 13). Bernard‑Soulier syndrome and platelet‑type von Willebrand disease are platelet function disorders that can be caused by mutations in GPIb alpha (1, 14).

Related Research Areas

• Coagulation
• Myeloid Lineage Markers
• Platelet Adhesion Molecules
• Platelet Markers