Home / CD46 / Recombinant Human CD46 His-tag Protein, CF

Recombinant Human CD46 His-tag Protein, CF

Catalog #: 10256-CD Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10256-CD-050
Recombinant Human CD46 His-tag Protein Binding Activity
2 Images
Product Details
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD46 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD46 is immobilized at 5 μg/mL (100 μL/well), the concentration Human C4b that produces 50% of the optimal binding response is 2-12 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human CD46 protein
Cys35-Asp328, with a C-terminal 10-His tag
Accession #
P15529-11
N-terminal Sequence
Analysis
Cys35
Predicted Molecular Mass
34 kDa
SDS-PAGE
40-67 kDa, under reducing conditions

Product Datasheets

You must select a language.

x

10256-CD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10256-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human CD46 His-tag Protein Binding Activity View Larger

When Recombinant Human CD46 (Catalog # 10256-CD) is immobilized at 5 μg/mL (100 μL/well), it binds to Human C4b with an ED50 of 2‑12 μg/mL.

SDS-PAGE Recombinant Human CD46 His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human CD46 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-67 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD46

CD46, also known as MCP (Membrane cofactor protein), is a type I membrane protein that functions as a cofactor for Complement Factor I­mediated inactivation of complement components C3b and C4b (1, 2). Mature human CD46 consists of a 309 amino acid (aa) extracellular domain (ECD) with four short consensus repeats (SCR) and a Ser/Thr/Pro-enriched region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain (1). Within the ECD, human CD46 shares 50% aa sequence identity with mouse and rat CD46. CD46 is commonly expressed in four distinct isoforms, and all isoforms have conserved complement control protein domains which harbor the C3b and C4b binding sites (3). CD46 is expressed on all nucleated cells (4). CD46 participates in the maintenance of immune homeostasis and the induction of protective T cell immunity, and it could extend towards key processes in development and cell metabolism (5).

References
  1. Liszewski, M.K. et al. (1991) Annu. Rev. Immunol. 9:431.
  2. Barilla-LaBarca, M.L. et al. (2002) J. Immunol. 168:6298.
  3. Yamamoto, H. et al. (2013) Intl. J. Biochem. Cell Biol. 45:2808.
  4. Andrews, P.W. et al. (1985) Ann. Hum. Genet. 49:31.
  5. Kolev, M. et al. (2013) Semin. Immunol. 25:12.
Entrez Gene IDs
4179 (Human); 17221 (Mouse)
Alternate Names
AHUS2; CD46 antigen; CD46 molecule, complement regulatory protein; CD46; MCP; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MIC10; TLXcomplement regulatory protein; trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; trophoblast-lymphocyte cross-reactive antigen

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human CD46 His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human CD46 His-tag Protein, CF and earn rewards!

Have you used Recombinant Human CD46 His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review