Recombinant Human CD68/SR-D1 His-tag Protein, CF Summary
Product Specifications
Asn22-Ser319, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10551-SR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Human CD68/SR-D1 His-tag Protein (Catalog # 10551-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing broad diffusive bands centered at 70-100 kDa.
Reconstitution Calculator
Background: CD68/SR-D1
CD68, also called Scavenger Receptor D1 (SR-D1) and LAMP-4, is a heavily glycosylated type I transmembrane glycoprotein that belongs to the LAMP family comprised of five lysosome-specific protein members including LAMP-1, LAMP-2, dendritic cell (DC)-LAMP (also known as CD208 and LAMP-3), and brain and dendritic cell-associated (BAD)-LAMP (also known as LAMP-5) (1, 2). CD68 expression represents the classical immunohistology marker molecule for human monocytes and tissue resident macrophages (3). It is mainly associated with endosomal/lysosomal compartment and serves as a scavenger receptor for oxidized LDL (4). In contrast to other members of the LAMP family of glycoproteins which are ubiquitously expressed in all cell types, CD68 appears to have a more restricted, cell-type specific pattern of expression (5). Human CD68 is expressed as a 354 amino acid (aa) protein, which includes a 21 aa signal peptide, a 298 aa extracellular domain (ECD), a 25 aa transmembrane segment, and a 10 aa cytoplasmic domain. Within the ECD, human CD68 shares 65% and 67% sequence identity with mouse and rat CD68, respectively. CD68 is a biomarker of rheumatoid arthritis and Hodgkin's lymphoma (6,7). In addition, CD68 on macrophages binds tightly to both S100A8 and S100A9 to enhance the cell immunity and influences the functions of cells through NF-kB/focal adhesion kinase pathway (8, 9).
- Chistiakov, D. et al. (2016) Lab. Invest. 97:4.
- Holness, C.L. and Simmons, D.L. (1993) Blood 81:1607.
- O’Reilly D. et al. (2007) Genomics 90:3.
- Ramprasad, M.P. et al. (1996) Proc. Natl. Acad. Sci. U. S. A. 93:14838.
- Gottfried, E. et al. (2008) Scand. J. Immunol. 67:453.
- Bresnihan, B. et al. (2009) J. Rheumatol. 36:1800.
- Touati, M. et al. (2015) Leuk. Lymphoma 56:332.
- Wang, H. et al. (2014) Cancer Lett. 345:91.
- Okada, K. et al. (2016) J. Leukoc. Biol. 100:1093.
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