Recombinant Human CD81 LEL Fc Chimera Protein, CF Summary
Product Specifications
| MD | Human IgG1 (Pro100-Lys330) |
IEGR | Human CD81 LEL |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9144-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human CD81 LEL Fc Chimera (Catalog # 9144-CD) is used at 2 µg/mL, Recombinant Viral HCV E2 (Catalog # 9146-HC) binds with an ED50 of 0.2-1.2 µg/mL.
Reconstitution Calculator
Background: CD81
CD81, also known as TAPA-1 and Tetraspanin-28, is an approximately 25 kDa palmitoylated component of plasma membrane lipid rafts (1). It contains four transmembrane segments, two extracellular loops of 30 and 90 amino acids (aa), and three short cytoplasmic regions (2, 3). Within the large extracellular loop, human CD81 shares 90% and 84% aa sequence identity with mouse and rat CD81, respectively. CD81 associates with a wide range of membrane proteins including CD151, TfR2, LDL R, PCSK9, Glypican 3, IFITM1, IGSF8/CD316, FPRP, and complexes of CD19-CD21 (4-11). It is required for the development of CD4+CD8+ DP thymocytes (12) and hepatocyte infection by Plasmodium sporozoites (13). It also supports B cell receptor signaling (11), Hepcidin expression (5), monocyte and B cell tethering to the vascular endothelium (14), and the immunosuppressive function of Treg and MDSC (15). CD81 additionally functions as a receptor for the E2 glycoprotein of hepatitis C virus (16). The CD81-E2 interaction inhibits NK cell cytolytic activity, provides a co-stimulatory signal to T cells, and inhibits the maturation of plasmacytoid dendritic cells (17-19).
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- Oren, R. et al. (1990) Mol. Cell. Biol. 10:4007.
- Levy, S. et al. (1991) J. Biol. Chem. 266:14597.
- Zhu, Y.-Z. et al. (2012) Virology 429:112.
- Chen, J. and C.A. Enns (2015) J. Biol. Chem. 290:7841.
- Le, Q.-T. et al. (2015) J. Biol. Chem. 290:23385.
- Takahashi, S. et al. (1990) J. Immunol. 145:2207.
- Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
- Stipp, C.S. et al. (2001) J. Biol. Chem. 276:4853.
- Liu, B. et al. (2009) Am. J. Pathol. 175:717.
- Cherukuri, A. et al. (2004) J. Immunol. 279:31973.
- Boismenu, R. et al. (1996) Science 271:198.
- Silvie, O. et al. (2003) Nat. Med. 9:93.
- Feigelson, S.W. et al. (2003) J. Biol. Chem. 278:51203.
- Vences-Catalan, F. et al. (2015) Cancer Res. 75:4517.
- Pileri, P. et al. (1998) Science 282:938.
- Crotta, S. et al. (2002) J. Exp. Med. 195:35.
- Tseng, C.-T. and G.R. Klimpel (2002) J. Exp. Med. 195:43.
- Tu, Z. et al. (2013) Cell. Immunol. 284:98.
Citation for Recombinant Human CD81 LEL Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Induction of cross-neutralizing antibodies by a permuted hepatitis C virus glycoprotein nanoparticle vaccine candidate
Authors: K Sliepen, L Radi?, J Capella-Pu, Y Watanabe, I Zon, A Chumbe, WH Lee, M de Gast, J Koopsen, S Koekkoek, I Del Moral-, PJM Brouwer, R Ravichandr, G Ozorowski, NP King, AB Ward, MJ van Gils, M Crispin, J Schinkel, RW Sanders
Nature Communications, 2022-11-25;13(1):7271.
Species: Human
Sample Types: Serum
Applications: ELISA Capture
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