Recombinant Human CD90/Thy1 His-tag Protein, CF

Catalog #: 10805-TH Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10805-TH-050

Recombinant Human CD90/Thy1 His-tag Protein SDS-PAGE.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD90/Thy1 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD90/Thy-1 His-tag is immobilized at 5.00 µg/mL (100 µL/well), Recombinant Human Galectin-1 (Catalog # 1152-GA) binds with an ED₅₀ of 0.400-2.00 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CD90/Thy1 protein
Gln20-Ser141, with a C-terminal 6-His tag. Accession # P04216.2

Accession #

P04216.2

N-terminal Sequence
Analysis

Gln20 inferred from enzymatic pyroglutamate treatment revealing Lys21.

Predicted Molecular Mass

14 kDa

SDS-PAGE

19-33 kDa, under reducing conditions.

Product Datasheets

10805-TH

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10805-TH

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human CD90/Thy1 His-tag Protein (Catalog # 10805-TH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19-33 kDa.

Reconstitution Calculator

Background: CD90/Thy1

CD90, also known as Thy1, is an approximately 25-35 kDa glycoprotein that mediates cellular adhesion and exerts wide ranging effects in different tissues (1, 2). CD90 is expressed on hematopoietic progenitor cells, neurons, and activated vascular endothelial cells (3-5). Its species-specific expression in lymphoid cells allows its use as a thymocyte and pan T cell marker in mouse but not in human (1). CD90 can form dimers and higher order multimers (6). It binds to heparin, the proteoglycan Syndecan-4, and Integrins alpha M beta 2, alpha V beta 3, and alpha V beta 5 (5, 7-10). Through these interactions, CD90 inhibits neurite outgrowth (4), promotes astrocyte adhesion (7), mediates the adhesion and extravasation of leukocytes and melanoma cells (5, 9), supports the Thrombospondin-1 induced disassembly of fibroblast focal adhesions (11), and inhibits the activation of latent TGF-beta 1, myofibroblast differentiation, and lung fibrosis (10). Human Thy-1 shares 63% and 66% amino acids similarity with mouse and rat homolog.

References