Recombinant Human CEACAM-18 Fc Chimera Protein, CF Summary
Product Specifications
0.5-5 μg/mL.
| Human CEACAM-18 (Gln31-His317) Accession # A8MTB9 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9869-CM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human Galectin-3 (Human Cell-expressed) (Catalog # 8259-GA) is coated at 1 µg/mL, 100 µL/well, Recombinant Human CEACAM-18 Fc Chimera (Catalog # 9869-CM) binds with an ED50 of 0.5-5 µg/mL.
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2 μg/lane of Recombinant Human CEACAM-18 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 93 -105 kDa and 190- 200 kDa, respectively.
Reconstitution Calculator
Background: CEACAM-18
Carcinoembryonic Antigen-related Cell Adhesion Molecule 18 (CEACAM-18) is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Mature human CEACAM-18 has a 298 amino acid (aa) extracellular domain containing 2 IgC2-like and 1 IgV-like domains, a single transmembrane domain and a short cytoplasmic tail (2). CEACAM-18 is one of only five conserved CEACAMs among mouse, rat, and human (2), but mature human CEACAM-18 has low aa sequence identify with mouse and rat at 60% and 58%, respectively. Originally discovered as a biomarker for colorectal cancer (3), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (4-6). While the exact function of CEACAM-18 has been yet to be elucidated, it may bind pathogen receptors or other immunoregulatory members (6). CEACAM family members were identified as the major Galectin-3 receptor candidates on human neutrophils (7). Binding of carbohydrate ligands to CEACAMs may be important in the release of proinflammatory mediators (8, 9).
- Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
- Zebhauser R. et al. (2005) Genomics 86:566.
- Gold P and Freedman SO, 1965) J Exp Med 122:467.
- Obrink, B. (1997) Curr Opin Cell Biol 9:616.
- Horst, A.K. and Wagener, C. (2004) Handb Exp Pharmacol 283.
- Kuespert K. et al. (2006) Curr Opin Cell Biol. 18(5):565.
- Feuk-Lagerstedt E. et al. (1999) J. Immunol. 163:5592.
- Yoon, J. et al. (2007) J. Immunol. 179:8454.
- Schröder, A.K. et al. (2006) Hum Immunol. 67:676.
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