Recombinant Human Chordin-Like 2 Protein, CF Summary
Product Specifications
Leu21-Thr429, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9127-CH
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl. |
| Reconstitution | Reconstitute at 500 μg/mL in 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Chordin-like 2/CHRDL2
Chordin-like 2 (CHL2, CHRDL2, or BNF-1), is an approximately 55 kDa secreted glycoprotein that acts as a BMP antagonist. Mature human CHL2 contains three von Willebrand type C repeats and shares 73% amino acid sequence identity with mouse and rat CHL2 (1-3). Complex alternative splicing of human Chordin-like 2 generates multiple short isoforms as well as a nearly full length isoform with a substituted C-terminus (3). CHL2 is expressed in chondrocytes in developing joint cartilage (2, 4), osteoblasts, myotubes, brain, some carcinomas, and the genitourinary system (e.g. uterus, prostate, testis, bladder) (1-3). CHL2 binds to multiple TGF-beta superfamily proteins including BMP-2, -4, -5, -6, -7, GDF-5, Activin A, and TSG (2, 3, 5, 6). It prevents the binding of BMPs to type 1 and type 2 receptors as well as BMP-induced cellular responses (2, 5). CHL2 reduces the rate of matrix deposition by mesenchymal cells, acting as a negative regulator of cartilage formation (2).
- Wu, I. and M.A. Moses (2003) Gene 311:105.
- Nakayama, N. et al. (2004) Development 131:229.
- Oren, A. et al. (2004) Gene 331:17.
- Lorda-Diez, C.I. et al. (2013) PLoS One 8:e60423.
- Fujisawa, T. et al. (2009) Biochem. Biophys. Res. Commun. 385:215.
- Zhang, J.L. et al. (2007) J. Biol. Chem. 282:20002.
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