Recombinant Human COCO Protein Summary
Product Specifications
Arg23-Ala189, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3047-CC
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
3047-CC/CF
| Formulation | Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: COCO
COCO, also known as DAND5, Dante, and CKTSF1B3, is a member of the DAN Domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and Cerberus (DAND4). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1 - 3). BMPs play important roles in tissue morphogenesis and development processes (4, 5, 6). The human COCO cDNA encodes a 189 amino acid (aa) precursor with a 22 aa signal sequence (2, 7). COCO has eight Cys residues in the cysteine-knot which places it in the CAN subfamily of BMP antagonists along with the other DAN family proteins (1). Human COCO shares 60% and 24% aa sequence identity with mouse and Xenopus COCO, respectively. It shares 17%, 20%, 25%, and 22% aa sequence identity with human DAN, Gremlin, PRDC, and Cerberus, respectively. In Xenopus embryonal development, COCO is expressed by pluripotent ectodermal cells. Expression is abruptly downregulated prior to gastrulation, and the loss of ectodermal cell pluripotency is coincident with COCO downregulation (7). COCO binds and inhibits Xnr1, BMP-4, Activin, and Wnt-8 (7). In mouse, COCO expression is elevated on the right side of Henson’s node at the early somite stage, in contrast to the left side expression of Nodal (8). COCO may cooperate with Nodal in gastrulation and embryonic left-right axis formation (5, 8).
- Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
- Katoh, M. and M. Katoh (2004) Oncol. Rep. 12:423.
- Katoh, M. and M. Katoh (2005) Int. J. Mol. Med. 15:885.
- Baron, M.H. (2005) Exp. Hematol. 33:1015.
- Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
- De Robertis, E.M. and H. Koruda (2004) Annu. Rev. Cell Dev. Biol. 20:285.
- Bell, E. et al. (2003) Development 130:1381.
- Pearce, J.J. et al. (1999) Dev. Biol. 209:98.
Citations for Recombinant Human COCO Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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ABCA4 Variant c.5714+5G>A in Trans With Null Alleles Results in Primary RPE Damage
Authors: Sajovic, J;Meglic, A;Corradi, Z;Khan, M;Maver, A;Vidmar, MJ;Hawlina, M;Cremers, FPM;Fakin, A;
Investigative ophthalmology & visual science
Species: Human
Sample Types: Transduced Whole Cells
Applications: Bioassay -
Correction of the Splicing Defect Caused by a Recurrent Variant in ABCA4 (c.769-784C>T) That Underlies Stargardt Disease
Authors: TZ Tomkiewicz, SE Nieuwenhui, FPM Cremers, A Garanto, RWJ Collin
Cells, 2022-12-07;11(24):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
COCO/DAND5 inhibits developmental and pathological ocular angiogenesis
Authors: N Popovic, E Hooker, A Barabino, A Flamier, F Provost, M Buscarlet, G Bernier, B Larrivée
Embo Molecular Medicine, 2021-02-15;0(0):e12005.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Identification and Rescue of Splice Defects Caused by Two Neighboring Deep-Intronic ABCA4 Mutations Underlying Stargardt Disease
Authors: S Albert, A Garanto, R Sangermano, M Khan, NM Bax, CB Hoyng, J Zernant, W Lee, R Allikmets, RWJ Collin, FPM Cremers
Am. J. Hum. Genet., 2018-03-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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COCO promote the formation of organoid
