Recombinant Human Collectrin Fc Chimera Protein, CF

Catalog #: 7580-CL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7580-CL-050

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Recombinant Human Collectrin Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit proliferation of mIMCD‑3 mouse epithelial cells. The ED₅₀ for this effect is 1.0-4.0 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Collectrin protein

Human Collectrin (Glu15-Pro141) Accession # Q9HBJ8	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9HBJ8

N-terminal Sequence
Analysis

Glu15

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

41 kDa (monomer)

SDS-PAGE

54-64 kDa, reducing conditions

Product Datasheets

7580-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7580-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Collectrin

Collectrin, also known as TMEM27 and NX‑17, is a 35‑45 kDa transmembrane protein that shows structural similarity to the C‑terminal region of Angiotensin Converting Enzyme 2 (ACE‑2) but lacks peptidase activity (1, 2). Mature human Collectrin consists of a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 60 aa cytoplasmic domain (3). Within the ECD, human Collectrin shares 87% and 89% aa sequence identity with mouse and rat Collectrin, respectively. Collectrin is expressed as a disulfide‑linked homodimer of variably glycosylated subunits (4‑6). It is highly expressed in the proximal convoluted tubules of renal collecting ducts and inhibits the proliferation of tubule epithelial cells (3, 6‑8). It associates with several amino acid transporters on the luminal surface of these cells, where it is required for the reabsorption of multiple amino acids (7‑9). Collectrin is also expressed in pancreatic islet beta cells and enhances glucose‑stimulated insulin secretion as well as insulin resistance (4, 5, 9, 10). In both the kidney and pancreas, cytoplasmic Collectrin interacts with multiple components of the SNARE complex and other vesicle trafficking proteins (5, 6). A 25 kDa glycosylated fragment of the ECD can be shed from both renal tubule cells and pancreatic beta cells (4, 10). In beta cells, shedding is mediated by BACE2, and the portion left in the membrane can be subsequently cleaved by gamma‑Secretase, releasing the intracellular domain into the cytosol (11). Inhibition of Collectrin shedding from beta cells results in increased beta cell mass and insulin secretion (11).

References