Recombinant Human Common beta Chain His-tag Protein, CF

Recombinant Human Common beta Chain His-tag Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Common beta Chain

The common beta chain (beta c) is also known as GM-CSF R beta (granulocyte-macrophage colony stimulating factor receptor beta subunit), IL-3 R beta, and IL-5 R beta (gene name CSF2RB and designated CD131) (1, 2). It is a 58-61 kDa type I transmembrane protein that associates with the ligand-specific IL-3 R alpha on T cells and other cells, IL-5 R alpha on eosinophils or GM-CSF R alpha on myeloid cells, to form high affinity receptor complexes (1, 2). The 897 amino acid (aa) human beta c contains a 16 aa signal sequence, a 427 aa extracellular domain (ECD) with two fibronectin type III domains, a transmembrane sequence, and a 437 aa cytoplasmic domain (2, 3). Within the ECD, human beta c shares 57-68% aa sequence identity with mouse, rat, equine, porcine, bovine and canine beta c. Complexes of GM-CSF with its specific alpha subunit then bind preformed beta c dimers, creating 2:2:2 hexamers that can combine to a dodecamer (4:4:4) (3). Beta c association and dimerization via the dimerization of the hexamer to the dodecamer is essential for JAK2 activation via GM-CSF signaling which imparts growth and survival signals (3). Except for eosinophils, beta c is primarily involved when rapid production of leukocytes is needed, rather than for developmental or steady-state cell production (1). Beta c also associates with other receptors, forming heteroreceptor complexes that allow beta c complexes to influence the signaling pathways activated by the associated receptor (1). Beta c thus enhances angiogenesis (when associated with VEGF R2/KDR/Flt-1 or beta 1 integrins), cell protection (with Erythropoietin R), and synergistic growth of stem cells (with SCF R/c- kit) (4-9). Defective production of beta c in humans is a cause of pulmonary alveolar proteinosis (10).

Related Research Areas

• Common beta Chain Receptor Family
• Cytokines and Receptors in Allergy and Asthma
• Cytokines and Receptors in Tfh Cell Differentiation and Function
• Monocyte Markers
• Receptors in the Jak/STAT Pathway