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Recombinant Human Contactin-3 Protein, CF

Catalog #: 5539-CN Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5539-CN-050
R&D Systems Recombinant Proteins and Enzymes
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Contactin-3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been pre-coated with 50 µL/well of the recombinant human Contactin-2 solution at 6-24 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Contactin-3 protein
Glu20-Pro992, with a C-terminal 10-His tag
Accession #
Q9P232
N-terminal Sequence
Analysis
Glu20
Predicted Molecular Mass
108.1 kDa
SDS-PAGE
140-150 kDa, reducing conditions

Product Datasheets

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5539-CN

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5539-CN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Contactin-3

Contactin-3, also known as BIG-1 and PANG, is a 150 kDa member of the TAG/F3 subgroup of Contactin neuronal adhesion proteins (1, 2). Mature human Contactin-3 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3, 4). Human Contactin-3 shares 92% aa sequence identity with mouse and rat Contactin-3. Contactin-3 is primarily expressed in the brain in discrete regions including the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (5, 6). Alternative splicing in rat may generate a soluble isoform (4). Mouse plasmacytoma cell lines produce multiple transcripts of Contactin-3 due to the presence of IAP proviruses (3). Immobilized Contactin-3 promotes neurite outgrowth from rat hippocampal neurons (4).

References
  1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
  2. Denisenko-Nehrbass, N. et al. (2002) J. Physiol. 96:99.
  3. Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
  4. Yoshihara, Y. et al. (1994) Neuron 13:415.
  5. Kamei, Y. et al. (2000) Genomics 69:113.
  6. Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.
Entrez Gene IDs
5067 (Human); 18488 (Mouse); 54279 (Rat)
Alternate Names
BIG-1; CNTN3; contactin 3 (plasmacytoma associated); Contactin3; Contactin-3; PANG; PCS

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