Recombinant Human Contactin-6 Fc Chimera Protein, CF

Catalog #: 7394-CN Datasheet / COA / SDS

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7394-CN-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Contactin-6 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. The ED₅₀ for this efffect is 1.0-4.0 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source

Chinese Hamster Ovary cell line, CHO-derived human Contactin-6 protein

Human Contactin-6 (Asp20-Ser891) Accession # Q9UQ52	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9UQ52

N-terminal Sequence
Analysis

Asp20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

122.9 kDa

SDS-PAGE

130-170 kDa, reducing conditions

Product Datasheets

7394-CN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7394-CN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Contactin-6

Contactin-6, also known as NB-3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPI‑linked neuronal adhesion proteins (1, 2). Mature human Contactin-6 consists of six immunoglobulin‑like domains (aa 26‑587), a flexible linker region, four fibronectin type III domains (aa 597‑992), and a GPI anchor (3). Within aa 20‑891, human Contactin-6 shares 89% aa sequence identity with mouse and rat Contactin-6. Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3‑7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1, PTPR alpha, and PTPR gamma as well as in trans with Notch-1 on oligodendrocytes (6, 8‑10). Its binding to Notch-1 triggers the release and translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5, 7).

References