Recombinant Human Corneodesmosin Protein, CF Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
Lys33-Pro528, with a C-terminal 6-His tag (Asp526Asn)
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5725-CN
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Corneodesmosin
Corneodesmosin, also known as CDSN and the S gene product, is a highly polymorphic secreted glycoprotein that plays an important structural role in the skin (1). It is expressed by differentiated keratinocytes in the corneal layer of the skin and is a major component of corneodesmosomes (2 - 4). It is also expressed in the inner root sheath of hair follicles (5, 6). Corneodesmosome has a high content of glycine, serine, and proline residues that promote its folding into a series of Gly-loop domains (2, 7). Corneodesmosin forms oligomers and associates homophilically to strengthen the adhesion between corneocytes (8, 9). Corneodesmosin-deficient mice exhibit a detachment of the corneal layer of the skin as well as hypotrichosis of the scalp and baldness (6, 10). Corneodesmosin is secreted by keratinocytes as a 52 - 56 kDa molecule which is then subjected to repeated sequential N- and C-terminal proteolysis (11). Species of 46, 43, 36, and 15 kDa are present in corneocytes (7, 11). Cleavage of the N-terminal Gly-loop diminishes Corneodesmosin’s ability to mediate adhesion, and this is a prerequisite for normal desquamation of the skin (8, 9). Reduced proteolysis of Corneodesmosin in psoriasis lesions is associated with the persistence of corneodesmosomes and scale retention (12). Premature truncation of Corneodesmosin is associated with hypotrichosis of the scalp (13).
- Pierard, G.E. et al. (2000) Int. J. Mol. Med. 6:217.
- Zhou, Y. and D.D. Chaplin (1993) Proc. Natl. Acad. Sci. 90:9470.
- Haftek, M. et al. (1997) Br. J. Dermatol. 137:864.
- Simon, M. et al. (1997) J. Biol. Chem. 272:31770.
- Mils, V. et al. (1992) J. Histochem. Cytochem. 40:1329.
- Matsumoto, M. et al. (2008) Proc. Natl. Acad. Sci. 105:6720.
- Guerrin, M. et al. (1998) J. Biol. Chem. 273:22640.
- Jonca, N. et al. (2002) J. Biol. Chem. 277:5024.
- Caubet, C. et al. (2004) J. Invest. Dermatol. 122:747.
- Leclerc, E.A. et al. (2009) J. Cell Sci. 122:2699.
- Simon, M. et al. (2001) J. Biol. Chem. 276:20292.
- Simon, M. et al. (2008) Br. J. Dermatol. 159:77.
- Levy-Nissenbaum, E. et al. (2003) Nat. Genet. 34:151.
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