Recombinant Human CUL4A/RBX1 Neddylated Complex Protein, CF
Recombinant Human CUL4A/RBX1 Neddylated Complex Protein, CF Summary
Product Specifications
Met1 - Ala759 with a N-terminal 10-His Sumo tag (CUL4A), Met1 - His108 (RBX1)
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E3-441
| Formulation | Supplied as a solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: CUL4A/RBX1 Complex
Cullin-4A (CUL4A) is a core component of multiple cullin-RING type E3
Ubiquitin ligase complexes that mediate the ubiquitination of proteins
involved in cell cycle progression, DNA repair and other processes. In
the DCX complex (DDB1-CUL4-X-box) CUL4A serves as a scaffold that
organizes the DDB1-X-box recognition subunits with the RBX1 subunit and
contributes to catalysis through positioning of the substrate and an E2
ubiquitin-conjugating enzyme. In vivo, the E3 ubiquitin ligase activity
of the DCX complex is dependent on neddylation of the cullin subunit,
though neddylation may be dispensable for some in vitro reactions.
- Baek K., et al. (2020) Nature 578: 461.
- Fischer E.S., et al. (2014) Nature 512: 49.
- He Y.J., et al. (2006) Genes Dev. 20: 2949.
- Ito T., et al. (2010) Science 327: 1345.
- Wang H., et al. (2006) Mol. Cell 22: 383.
Citation for Recombinant Human CUL4A/RBX1 Neddylated Complex Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Targeted Protein Degradation through Recruitment of the CUL4A Complex Adaptor Protein DDB1
Authors: Meyers, M;Cismoski, S;Panidapu, A;Chie-Leon, B;Nomura, DK;
bioRxiv : the preprint server for biology
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay
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