Recombinant Human CUL5/RBX2 Complex Protein, CF

Recombinant Human CUL5/RBX2 Complex Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CUL5/RBX2 Complex

Cullin-5 (CUL5) is a core component of multiple ECS (Elongin-CUL5-SOCS-box protein) E3 ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cytokine receptor signal transduction.  In the ECS complex, CUL5 serves as a scaffold that organizes the Elongin B/Elongin C/SOCS (Suppressor of Cytokine Signaling) recognition subunits with the RBX subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the ECS complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  This complex consists of an N-terminal 10-His tagged CUL5 (UniProt Q93034) and untagged RBX2 (UniProt Q9UBF6).

Related Research Areas

• Cell Cycle
• Deubiquitinating Enzymes (DUBs)
• Search Ubiquitin-related Enzymes
• Toll-Like Receptors