Recombinant Human CXCL5/ENA-74 Protein Summary
Product Specifications
Ala41-Asn114
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
651-NA
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
651-NA/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CXCL5/ENA-74
CXCL5, also known as epithelial cell-derived neutrophil-activating peptide (ENA-78), is an 8 kDa proinflammatory member of the CXC subfamily of chemokines. Its Glu-Leu-Arg (ELR) motif confers angiogenic properties and distinguishes it from ELR-CXC chemokines which are angiostatic (1-3). Human CXCL5 shares 57% amino acid (aa) sequence identity with mouse and rat CXCL5. Among other human ELR+ chemokines, it shares 77% aa sequence identity with CXCL6/GCP-2 and
35%-51% with CXCL1/GRO alpha, CXCL2/GRO beta, CXCL3/GRO gamma, CXCL7/NAP-2, and CXCL8/IL-8. Inflammatory stimulation up-regulates CXCL5 production in multiple hematopoietic cell types, fibroblasts, endothelial cells, and vascular smooth muscle cells. In vivo, CXCL5 is elevated at sites of inflammation and pulmonary fibrosis where it promotes neutrophil infiltration and activation as well as angiogenesis (3-6). Its up-regulation contributes to increased vascularization, tumor growth, and metastasis in many cancers (6-9). Full length CXCL5 (78 aa) is trimmed at the N-terminal end by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70 aa), with the shortened forms showing increased potency relative to full length CXCL5 (10, 11). CXCL5 exerts its effects primarily through interactions with CXCR2 (6, 12). It also binds DARC, a decoy chemokine receptor which can limit CXCR2-mediated responses (13, 14).
- Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
- Balestrieri, M.L. et al. (2008) Cardiovasc. Res. 78:250.
- Walz, A. et al. (1991) J. Exp. Med. 174:1355.
- Strieter, R.M. et al. (1992) Immunol. Invest. 21:549.
- Koch, A.E. et al. (1994) J. Clin. Invest. 94:1012.
- Begley, L.A. et al. (2008) Neoplasia 10:244.
- Vandercappellen, J. et al. (2008) Cancer Lett. 267:226.
- Arenberg, D.A. et al. (1998) J. Clin. Invest. 102:465.
- Miyazaki, H. et al. (2006) Cancer Res. 66:4279.
- Wuyts, A. et al. (1999) Eur. J. Biochem. 260:421.
- Nufer, O. et al. (1999) Biochemistry 38:636.
- Ahuja, S.K. and P.M. Murphy (1996) J. Biol. Chem. 271:20545.
- Kashiwazaki, M. et al. (2003) Int. Immunol. 15:1219.
- Horton, L.W. et al. (2007) Cancer Res. 67:9791.
Citations for Recombinant Human CXCL5/ENA-74 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8.
Authors: Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW
J. Biol. Chem., 2006-12-29;282(16):11658-66.
Species: Mouse
Sample Types: Complex Sample Type
Applications: Binding Assay
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