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Recombinant Human CXCL5/ENA-78 Protein

Catalog #: 254-XB
9 Citations 2 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
254-XB-025/CF

With Carrier

Catalog # Availability Size / Price Qty
254-XB-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (9)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CXCL5/ENA-78 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 is 3-15 ng/mL
Source
E. coli-derived human CXCL5/ENA-78 protein
Ala37-Asn114
Accession #
P42830
N-terminal Sequence
Analysis
Ala37
Predicted Molecular Mass
8.4 kDa
SDS-PAGE
7 kDa, reducing conditions

Product Datasheets

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254-XB (with carrier)

Product Datasheet
COA
SDS

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254-XB/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

254-XB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS containing BSA as carrier protein
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

254-XB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL5/ENA-78

CXCL5, also known as epithelial cell-derived neutrophil-activating peptide (ENA-78), is an 8 kDa proinflammatory member of the CXC subfamily of chemokines. Its Glu-Leu-Arg (ELR) motif confers angiogenic properties and distinguishes it from ELR-CXC chemokines which are angiostatic (1-3). Human CXCL5 shares 57% amino acid (aa) sequence identity with mouse and rat CXCL5. Among other human ELR+ chemokines, it shares 77% aa sequence identity with CXCL6/GCP-2 and 35%-51% with CXCL1/GRO alpha, CXCL2/GRO beta, CXCL3/GRO gamma, CXCL7/NAP-2, and CXCL8/IL-8. Inflammatory stimulation up-regulates CXCL5 production in multiple hematopoietic cell types, fibroblasts, endothelial cells, and vascular smooth muscle cells. In vivo, CXCL5 is elevated at sites of inflammation and pulmonary fibrosis where it promotes neutrophil infiltration and activation as well as angiogenesis (3-6). Its up-regulation contributes to increased vascularization, tumor growth, and metastasis in many cancers (6-9). Full length CXCL5 (78 aa) is trimmed at the N-terminus by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70 aa), with the shortened forms showing increased potency relative to full length CXCL5 (10, 11). CXCL5 exerts its effects primarily through interactions with CXCR2 (6, 12). It also binds duffy antigen receptor for chemokines (DARC), which can limit CXCR2-mediated responses (13, 14).

References
  1. Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
  2. Balestrieri, M.L. et al. (2008) Cardiovasc. Res. 78:250.
  3. Walz, A.. et al. (1991) J. Exp. Med. 174:1355.
  4. Strieter, R.M. et al. (1992) Immunol. Invest. 21:549.
  5. Koch, A.E. et al. (1994) J. Clin. Invest. 94:1012.
  6. Begley, L.A. et al. (2008) Neoplasia 10:244.
  7. Vandercappellen, J. et al. (2008) Cancer Lett. 267:226.
  8. Arenberg, D.A. et al. (1998) J. Clin. Invest. 102:465.
  9. Miyazaki, H. et al. (2006) Cancer Res. 66:4279.
  10. Wuyts, A. et al. (1999) Eur. J. Biochem. 260:421.
  11. Nufer, O. et al. (1999) Biochemistry 38:636.
  12. Ahuja, S.K. and P.M. Murphy (1996) J. Biol. Chem. 271:20545.
  13. Kashiwazaki, M. et al. (2003) Int. Immunol. 15:1219.
  14. Horton, L.W. et al. (2007) Cancer Res. 67:9791.
Entrez Gene IDs
6374 (Human)
Alternate Names
chemokine (C-C motif) ligand 5; C-X-C motif chemokine 5; CXCL5; CXCL5/ENA-78; ENA78; ENA-78; ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; SCYB5ENA78

Citations for Recombinant Human CXCL5/ENA-78 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. CXCL5 suppression recovers neovascularization and accelerates wound healing in diabetes mellitus
    Authors: Chen, C;Lin, LY;Chen, JW;Chang, TT;
    Cardiovascular diabetology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. CXCL5 promotes mitomycin C resistance in non-muscle invasive bladder cancer by activating EMT and NF-?B pathway
    Authors: C Wang, A Li, S Yang, R Qiao, X Zhu, J Zhang
    Biochem. Biophys. Res. Commun., 2018-03-17;0(0):.
    Species: Human
    Sample Types: RT4 cells
    Applications: Bioassay
  3. Complex cellular composition of solitary fibrous tumor of the prostate.
    Authors: Gharaee-Kermani M, Mehra R, Robinson D, Wei J, Macoska J
    Am J Pathol, 2014-01-13;184(3):732-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. CXCL5 stimulation of RANK ligand expression in Paget's disease of bone.
    Authors: Sundaram K, Rao D, Ries W, Reddy S
    Lab Invest, 2013-02-25;93(4):472-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Interleukin-33 overexpression is associated with gamma-glutamyl transferase in biliary atresia.
    Cytokine, 2012-11-22;61(2):433-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
    EMBO J., 2012-07-31;31(17):3607-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Overexpression of CXCL5 is associated with poor survival in patients with pancreatic cancer.
    Authors: Li A, King J, Moro A, Sugi MD, Dawson DW, Kaplan J, Li G, Lu X, Strieter RM, Burdick M, Go VL, Reber HA, Eibl G, Hines OJ
    Am. J. Pathol., 2011-03-01;178(3):1340-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Adipose tissue-derived stem cells secrete CXCL5 cytokine with chemoattractant and angiogenic properties.
    Authors: Zhang H, Ning H, Banie L, Wang G, Lin G, Lue TF, Lin CS
    Biochem. Biophys. Res. Commun., 2010-10-27;402(3):560-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human CXCL5/ENA-78 Protein

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Recombinant Human CXCL5/ENA-78 Protein
By Anonymous on 08/23/2018
Application: Immunoassay Standard

Used with MAB654 in an immunoassay.

Immunoassay Standard Recombinant Human CXCL5/ENA-78 Protein 254-XB
Recombinant Human CXCL5/ENA-78 Protein
By Jennifer Taggart on 08/20/2018
Application: In vitro bioactivity in cell culture
Reason for Rating: Easy to follow kit and always high quality.