Home / Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

Catalog #: 11265-DC
1 Citation Datasheet / COA / SDS
CHO-Expressed
Catalog # Availability Size / Price Qty
11265-DC-050
Recombinant Human DC-SIGN/CD209 Fc Chimera Protein Bioactivity.
2 Images
Product Details
Citations (1)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED50 for this effect is 0.700-7.00 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human DC-SIGN/CD209 protein
MDHuman IgG1
(Pro100-Lys330)		IEGRHuman DC-SIGN/CD209
(Lys62-Ala404)
Accession # Q9NNX6.1
N-terminusC-terminus
Accession #
Q9NNX6.1
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
65.7 kDa
SDS-PAGE
62-74 kDa, under reducing conditions.

Product Datasheets

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11265-DC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11265-DC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Recombinant Human DC‑SIGN/CD209 Fc Chimera (Catalog # 11265-DC) supports the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED50 for this effect is 0.700-7.00 µg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human DC‑SIGN/CD209 Fc Chimera Protein (Catalog # 11265-DC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-74 kDa and 120-150 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background:

Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (5, 6).

References
  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
  4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
  5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
  6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Citation for Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Recognition of highly branched N-glycans of the porcine whipworm by the immune system
    Authors: Eckmair, B;Gao, C;Mehta, AY;Dutkiewicz, Z;Vanbeselaere, J;Cummings, RD;Paschinger, K;Wilson, IBH;
    bioRxiv : the preprint server for biology
    Species: N/A
    Sample Types: Proteins
    Applications: Bioassay

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