Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF

Catalog #: 142-DC
11 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
142-DC-100

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Citations (11)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759. The ED₅₀ for this effect is 0.03‑0.15 µg/mL in the presence of 20 ng/mL rhFas Ligand.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DcR3/TNFRSF6B protein

Human DcR3 (Val 24-His300) Accession # O95407	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

O95407

N-terminal Sequence
Analysis

Val24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

57 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Product Datasheets

142-DC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

142-DC

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IAP Inhibitors

Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNFRII (29%) and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2 and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Overexpression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.

References

Pitti, R.M. et al. (1998) Nature 396:699.
Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733.
Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA. 97:1230.

Alternate Names

IAP Inhibitors

Related Research Areas

Citations for Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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A stable, engineered TL1A ligand co-stimulates T cells via specific binding to DR3
Authors: A Zwolak, SR Chan, P Harvilla, S Mahady, AA Armstrong, L Luistro, N Tamot, D Yamada, M Derebe, S Pomerantz, M Chiu, R Ganesan, P Chowdhury
Scientific Reports, 2022-11-29;12(1):20538.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Decoy Receptor 3 Promotes Preosteoclast Cell Death via Reactive Oxygen Species-Induced Fas Ligand Expression and the IL-1alpha/IL-1 Receptor Antagonist Pathway
Authors: YJ Peng, CT Peng, YH Lin, GJ Lin, SH Huang, SJ Chen, HK Sytwu, CP Cheng
Mediators Inflamm., 2020-06-10;2020(0):1237281.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Immunological Properties of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells
Authors: M Idelson, R Alper, A Obolensky, N Yachimovic, J Rachmilewi, A Ejzenberg, E Beider, E Banin, B Reubinoff
Stem Cell Reports, 2018-08-16;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
MAbs, 2018-03-05;0(0):1-43.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Capture
DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1.
BMC Cancer, 2012-05-14;12(0):176.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
Authors: Tso GH, Law HK, Tu W
Stem Cells, 2010-05-01;28(5):939-54.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Creation of a LIGHT mutant with the capacity to evade the decoy receptor for cancer therapy.
Authors: Morishige T, Yoshioka Y, Inakura H, Tanabe A, Yao X, Tsunoda S, Tsutsumi Y, Mukai Y, Okada N, Nakagawa S
Biomaterials, 2010-02-01;31(12):3357-63.
Applications: Surface Plasmon Resonance
Soluble decoy receptor 3 modulates the survival and formation of osteoclasts from multiple myeloma bone disease patients.
Authors: Colucci S, Brunetti G, Mori G, Oranger A, Centonze M, Mori C, Cantatore FP, Tamma R, Rizzi R, Liso V, Zallone A, Grano M
Leukemia, 2009-07-09;23(11):2139-46.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
The Decoy Receptor 3 (DcR3, TNFRSF6B) suppresses Th17 immune responses and is abundant in human cerebrospinal fluid.
Authors: Mueller AM, Pedre X, Killian S, David M, Steinbrecher A
J. Neuroimmunol., 2009-03-06;209(1):57-64.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts.
Authors: Nakamichi Y, Udagawa N, Kobayashi Y, Nakamura M, Yamamoto Y, Yamashita T, Mizoguchi T, Sato M, Mogi M, Penninger JM, Takahashi N
J. Immunol., 2007-01-01;178(1):192-200.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis.
Authors: Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A
Arthritis Rheum., 2006-05-01;54(5):1451-62.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay