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Recombinant Human DDB1 Protein, CF

Catalog #: E3-501 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
E3-501-050

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Recombinant Human DDB1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DDB1 protein
Met1 - His1140
Accession #
Q16531.1
Predicted Molecular Mass
127 kDa

Product Datasheets

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E3-501

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-501

Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Background: DDB1

DNA damage-binding protein 1 (DDB1) is a component of the UV-damaged DNA binding (UV-DDB) complex which minimally consists of DDB1 and DDB2.  At 1,140 amino acids in length and a predicted molecular weight of 127 kDa, human DDB1 is 99% identical to both its mouse and rat orthologues.  DDB1 is also a component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin Ligase complexes where it serves to associate the Cullin-4 subunit to substrate recognition subunits such as Cereblon (CRBN), and various DCAF (DDB1-CUL4 Associate Factor) proteins.  

References
  1. Fischer E.S. et al. (2014) Nature 512: 49.
  2. He Y.J., et al. (2006) Genes Dev.  20: 2949.
  3. Ito T., et al. (2010) Science 327: 1345.
  4. Wang H., et al. (2006) Mol. Cell  22: 383.

Long Name
Damage-specific DNA Binding Protein 1
Entrez Gene IDs
1642 (Human); 13194 (Mouse); 64470 (Rat)
Alternate Names
damage-specific DNA binding protein 1 (127kD); damage-specific DNA binding protein 1, 127kDa; Damage-specific DNA-binding protein 1; DDB p127 Subunit; DDB1; DDBA; DNA damage-binding protein 1; DNA damage-binding protein a; HBV X-associated protein 1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; UV-DDB1; XAP1; XAP-1; Xeroderma pigmentosum group E-complementing protein; XPCE; XPE; XPE-BF; XPE-binding factor

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