Recombinant Human DEC-205/CD205 Fc Chimera Protein, CF

Catalog #: 10205-DE Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10205-DE-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DEC-205/CD205 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD302/CLEC13A Fc Chimera (Catalog # 10203-CL) is immobilized at 5 µg/mL (100 µL/well), Recombinant Human DEC-205/CD205 Fc Chimera (Catalog # 10205-DE) binds with an ED₅₀ of 4-28 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human DEC-205/CD205 protein

Human DEC-205/CD205 (Ser28-Asp1666) Accession # O060449.3	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

O60449.3

N-terminal Sequence
Analysis

Ser28

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

215 kDa

SDS-PAGE

200-250 kDa, under reducing conditions

Product Datasheets

10205-DE

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10205-DE

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant CD302/CLEC13A Fc Chimera (10203-CL) is immobilized at 5 µg/mL, 100 µL/well, Recombinant Human DEC-205/CD205 Fc Chimera (Catalog # 10205-DE) binds with an ED₅₀ of 4-28 µg/mL.

Reconstitution Calculator

Background: DEC-205/CD205

DEC-205, also known as CD205 and lymphocyte antigen 75 (Ly75), is a type I transmembrane protein that is part of the macrophage mannose receptor (MMR) family of C-type lectins (1). Both the human and mouse proteins share a similar extracellular (ECD) structure consisting of a cysteine rich N-terminal domain, a fibronectin type II domain, and multiple C-type lectin-like domains (1, 2). The mature ECD of human DEC-205 shares 68% amino acid (aa) identity with mouse DEC‑205. DEC‑205 is primarily expressed on dendritic cells (DC) of lymphoid tissues and at lower levels on macrophages and T cells (3, 4). DEC-205 functions as an endocytic receptor for antigens (3, 4). DEC-205 was shown to be a a cell surface receptor for CpG-oligonucleotides (ODN), as mice deficient in DEC-205 have impaired DC and B-cell maturation (5). DEC-205 expressing Kupffer stellate macrophage cells in the liver responded to CpG-ODN and subsequently released cytokines to promote NKT cell activation, leading to liver damage (6). DEC-205 also recognizes apoptotic and necrotic cells by binding to keratin in a pH-dependent fashion, leading to either immune activation or tolerance in the absence or presence of inflammatory stimuli (7, 8). DEC-205 positive macrophages in the human gastric mucosa were shown to mediate local and systemic immune responses to H. pylori infection, which affects systemic immunity (9). DEC-205 was also found to exist as an intergenic splice variant able to form a fusion protein with CD302 in Hodgkin's lymphoma cell lines (10). The DEC-205/CD302 fusion protein was also found to be expressed by mature dendritic cells which altered endocytic capacity of DEC-205, although the wild-type single gene transcripts were the dominant isoforms expressed (11). DEC-205 has been widely used in immune therapies for vaccine generation. Targeting of antigens to DEC-205 resulted in enhanced antigen presentation by DC. In combination with adjuvants, proteins coupled to antibodies against DEC-205 induced strong pathogen-specific immune responses, while without additional adjuvant tolerance could be induced (12).

References