Home / Decorin / Recombinant Human Decorin Protein, CF

Recombinant Human Decorin Protein, CF

Catalog #: 143-DE
16 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
143-DE-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (16)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Decorin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to modulate collagen fibrillogenesis. Ge, G. et al. (2004) J. Biol. Chem. 279:41626. At 5 µg/mL, Recombinant Human Decorin can significantly delay the rate of type I collagen fibrillogenesis.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Decorin protein
Gly17-Lys359
Accession #
P07585
N-terminal Sequence
Analysis
Gly17
Predicted Molecular Mass
38 kDa
SDS-PAGE
40-42 kDa, reducing conditions

Product Datasheets

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143-DE

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

143-DE

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Decorin

Decorin, also known as PG40 and PGS2, is a secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine‑rich proteoglycans (SLRPs). SLRP family members are characterized by N‑terminal and C‑terminal cysteine‑rich regions which flank the central region containing 10 ‑ 12 tandem leucine‑rich repeats (LRR) (1). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide (2). Mature human Decorin contains twelve tandem LRR and shares 80% and 78% aa sequence identity with mouse and rat Decorin, respectively. Alternate splicing of human Decorin generates five isoforms with variable length deletions. Decorin is an N‑glycosylated protein that also carries a variably‑sized hybrid chondroitin/dermatan sulfate chain at Ser34 (3, 4). Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa (5). Decorin regulates assembly of the extracellular collagen matrix and the bioactivity of the matrix associated growth factors FGF‑2, GDF‑8/Myostatin, TGF‑ beta, and WISP‑1 (4, 6 ‑ 9). It also binds and activates EGF R, ErbB4, and IGF‑I R (10 ‑ 12). In vivo, Decorin promotes myoblast differentiation, supports angiogenesis, and inhibits tumor progression (13 ‑ 16). Decorin is cleared from the extracellular space by LRP‑mediated endocytosis (17).

References
  1. Schaefer, L. and R.V. Iozzo (2008) J. Biol. Chem. 283:21305.
  2. Krusius, T. and E. Ruoslahti (1986) Proc. Natl. Acad. Sci. 83:7683.
  3. Scholzen, T. et al. (1994) J. Biol. Chem. 269:28270.
  4. Zamfir, A. et al. (2003) Glycobiology 13:733.
  5. Roughley, P.J. and R.J. White (1989) Biochem. J. 262:823.
  6. Ferdous, Z. et al. (2007) J. Biol. Chem. 282:35887.
  7. Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
  8. Cabello-Verrugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842.
  9. Desnoyers, L. et al. (2001) J. Biol. Chem. 276:47599.
  10. Zhu, J.-X. et al. (2005) J. Biol. Chem. 280:32468.
  11. Santra, M. et al. (2000) J. Biol. Chem. 275:35153.
  12. Schonherr, E. et al. (2005) J. Biol. Chem. 280:15767.
  13. Olguin, H.C. et al. (2003) Dev. Biol. 259:209.
  14. Schonherr, E. et al. (2004) J. Vasc. Res. 41:499.
  15. Seidler, D.G. et al. (2006) J. Biol. Chem. 281:26408.
  16. Reed, C.C. et al. (2005) Oncogene 24:1104.
  17. Brandan, E. et al. (2006) J. Biol. Chem. 281:31562.
    Entrez Gene IDs
    1634 (Human); 13179 (Mouse)
    Alternate Names
    Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B

    Citations for Recombinant Human Decorin Protein, CF

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    16 Citations: Showing 1 - 10
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    1. Cystine-knot peptide inhibitors of HTRA1 bind to a cryptic pocket within the active site region
      Authors: Li, Y;Wei, Y;Ultsch, M;Li, W;Tang, W;Tombling, B;Gao, X;Dimitrova, Y;Gampe, C;Fuhrmann, J;Zhang, Y;Hannoush, RN;Kirchhofer, D;
      Nature communications
      Species: N/A
      Sample Types: Peptide, Recombinant Protein
      Applications: Bioassay
    2. Decorin: a potential therapeutic candidate for ligamentum flavum hypertrophy by antagonizing TGF-beta 1
      Authors: Wang, S;Qu, Y;Fang, X;Ding, Q;Zhao, H;Yu, X;Xu, T;Lu, R;Jing, S;Liu, C;Wu, H;Liu, Y;
      Experimental & molecular medicine
      Species: Human, Rat
      Sample Types: In Vivo, Whole Cells
      Applications: Bioassay, In Vivo
    3. Allosteric inhibition of HTRA1 activity by a conformational lock mechanism to treat age-related macular degeneration
      Authors: S Gerhardy, M Ultsch, W Tang, E Green, JK Holden, W Li, A Estevez, C Arthur, I Tom, A Rohou, D Kirchhofer
      Nature Communications, 2022-09-05;13(1):5222.
      Species: Human
      Sample Types: Recombinant Protein
      Applications: Bioassay
    4. The Preventive Effect of Decorin on Epidural Fibrosis and Epidural Adhesions After Laminectomy
      Authors: Q Ding, Q Wei, G Sheng, S Wang, S Jing, T Ma, R Zhang, T Wang, W Li, X Tang, H Wu, C Liu
      Frontiers in Pharmacology, 2021-12-16;12(0):774316.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    5. Decorin inhibits glucose-induced lens epithelial cell apoptosis via suppressing p22phox-p38 MAPK signaling pathway
      Authors: S Du, J Shao, D Xie, F Zhang
      PLoS ONE, 2020-04-27;15(4):e0224251.
      Species: Human
      Sample Types: Whole Cellls
      Applications: Cell Culture
    6. Anti-fibrotic effect of decorin in peritoneal dialysis and PD-associated peritonitis
      Authors: N Jiang, Q Zhang, MK Chau, MS Yip, SL Lui, S Liu, KM Chu, HY Ngan, TM Chan, S Yung
      EBioMedicine, 2020-02-12;52(0):102661.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    7. Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment
      Authors: AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard
      Breast Cancer Res., 2019-02-27;21(1):31.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    8. Topical small molecule�granzyme B�inhibitor improves remodeling in a�murine model of impaired burn wound healing
      Authors: Y Shen, MR Zeglinski, CT Turner, SA Raithatha, Z Wu, V Russo, C Oram, S Hiroyasu, L Nabai, H Zhao, T Bozin, K Westendorf, I Kopko, R Huang, S Arns, J Tan, H Zeng, A Boey, R Liggins, J Jaquith, DR Cameron, A Papp, DJ Granville
      Exp. Mol. Med., 2018-05-30;50(5):68.
      Species: Mouse
      Sample Types: In Vivo, Recombinant Protein
      Applications: Bioassay, In Vivo
    9. Investigating Colorimetric Protein Array Assay Schemes for Detection of Recurrence of Bladder Cancer
      Authors: S Gogalic, U Sauer, S Doppler, C Preininger
      Biosensors (Basel), 2018-01-24;8(1):.
      Species: Human
      Sample Types: Urine
      Applications: Array Development
    10. Heat shock protein 47 and 65 KDa FK506 binding protein weakly but synergistically interact during collagen folding in the endoplasmic reticulum
      Authors: Y Ishikawa, P Holden, HP Bachinger
      J. Biol. Chem., 2017-08-31;0(0):.
      Applications: Control
    11. Platelet-rich plasma, especially when combined with a TGF-beta inhibitor promotes proliferation, viability and myogenic differentiation of myoblasts in vitro.
      Authors: Kelc R, Trapecar M, Gradisnik L, Rupnik M, Vogrin M
      PLoS ONE, 2015-02-13;10(2):e0117302.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    12. Lytic and mechanical stability of clots composed of fibrin and blood vessel wall components.
      Authors: Rottenberger Z, Komorowicz E, Szabo L, Bota A, Varga Z, Machovich R, Longstaff C, Kolev K
      J Thromb Haemost, 2013-03-01;11(3):529-38.
      Species: Bovine
      Sample Types: Whole Tissue
      Applications: Bioassay
    13. Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking.
      Authors: Zhang Y, Conrad AH, Conrad GW
      J. Biol. Chem., 2011-02-18;286(15):13011-22.
      Species: Bovine
      Sample Types: Whole Cells
      Applications: Bioassay
    14. Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium.
      Authors: Kitaya K, Yasuo T
      J. Leukoc. Biol., 2008-12-16;85(3):391-400.
      Applications: Antibody Absorption
    15. Decorin is a secreted protein associated with obesity and type 2 diabetes.
      Authors: Bolton K, Segal D, McMillan J, Jowett J, Heilbronn L, Abberton K, Zimmet P, Chisholm D, Collier G, Walder K
      Int J Obes (Lond), 2008-04-15;32(7):1113-21.
      Applications: ELISA (Standard)
    16. Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
      Authors: Barkho BZ, Song H, Aimone JB
      Stem Cells Dev., 2006-06-01;15(3):407-21.
      Species: Rat
      Sample Types: Whole Cells
      Applications: Bioassay

    FAQs

    1. What isoform is your Recombinant Human Decorin Protein (catalog #: 143-DE)?

      • The sequence of this protein reflects that of the canonical isoform A, accession P07585-1.

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