Recombinant Human Desmocollin-3 Protein, CF Summary
Product Specifications
Arg136-Lys690, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4630-DC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Desmocollin-3
Desmocollin-3 is a 100 - 110 kDa type I transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1 - 3). The human Desmocollin-3 is synthesized with a 108 amino acid (aa) propeptide. The mature protein consists of a 555 aa extracellular domain (ECD) that contains five cadherin-like domains, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain (4, 5). Within the ECD, human Desmocollin-3 shares 78% aa sequence identity with mouse and rat Desmocollin-3. It shares 52% and 64% aa sequence identity with Desmocollin-1 and -2, respectively. A splice variant of human Desmocollin-3 has a truncated and substituted cytoplasmic domain (4, 6). Desmocollin-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (5, 7, 8). Desmocollin-1 and -2, by contrast, are preferentially localized in the more superficial layers (7). In mouse, Desmocollin-3 is expressed throughout embryogenesis and is required for non-desmosomal functions during early development (9, 10). During apoptosis, both isoforms of Desmocollin-3 are proteolytically cleaved by caspases and metalloproteases (11). Desmocollin-3 can be upregulated or downregulated in carcinoma (6, 12, 13).
- Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
- Garrod, D.R. et al. (2002) Mol. Memb. Biol. 19:81.
- Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
- Kawamura, K. et al. (1994) J. Biol. Chem. 269:26295.
- King, I.A. et al. (1995) J. Invest. Dermatol. 105:314.
- Klus, G.T. et al. (2001) Int. J. Oncol. 19:169.
- North, A.J. et al. (1996) Proc. Natl. Acad. Sci. 93:7701.
- Nuber, U.A. et al. (1996) Eur. J. Cell Biol. 71:1.
- Chidgey, M.A.J. et al. (1997) Dev. Dyn. 210:315.
- Den, Z. et al. (2006) J. Cell Sci. 119:482.
- Weiske, J. et al. (2001) J. Biol. Chem. 276:41175.
- Wang, L. et al. (2007) Virchows Arch. Sept. 11 epub.
- Boelens, M.C. et al. (2007) J. Clin. Pathol. 60:608.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human Desmocollin-3 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Desmocollin-3 Protein, CF and earn rewards!
Have you used Recombinant Human Desmocollin-3 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
