Recombinant Human Desmoglein-4 Fc Chimera Protein, CF Summary
Product Specifications
| Human Desmoglein-4 (Glu24-Ala631) Accession # Q86SJ6 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9230-DM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Immobilized Recombinant Human Desmoglein‑4 Fc Chimera (Catalog # 9230-DM) supports the adhesion of A431 human epithelial carcinoma cells. The ED50 for this effect is 0.4-2.4 μg/mL.
Reconstitution Calculator
Background: Desmoglein-4
Desmoglein-4 is an approximately 140 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. Human Desmoglein-4 is synthesized with a 26 amino acid (aa) propeptide followed by a 582 aa extracellular domain (ECD) with four tandem cadherin-like domains, a 21 aa transmembrane segment, and a 388 aa cytoplasmic domain (1, 2). Within the ECD, human Desmoglein-4 shares 82% aa sequence identity with mouse and rat Desmoglein-4. Alternative splicing generates additional isoform with a substitution in the cytoplasmic domain. Desmoglein-4 is the principal cadherin of desmosomes which form adhesive contacts between epithelial cells (2, 3). It is expressed in hair follicles and differentiated keratinocytes in suprabasal layers of the skin and plays a role in keratinocyte differentiation and adhesion (2, 3). Mutations in human Desmoglein-4 are associated with the development of monilethrix autosomal recessive hypotrichosis (LAH) (2, 4-8). Autoantibodies directed against Desmoglein-3 in the blistering skin disorder pemphigus vulgaris can cross-react with Desmoglein-4 (2, 9, 10).
- Whittock, N.V. and C. Bower (2003) J. Invest. Dermatol. 120:523.
- Kljuic, A. et al. (2003) Cell 113:249.
- Bazzi, H. et al. (2006) Differentiation 74:129.
- Bazzi, H. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:222.
- Schaffer, J.V. et al. (2006) J. Invest. Dermatol. 126:1286.
- Shimomura, Y. et al. (2006) J. Invest. Dermatol. 126:1281.
- Zlotogorski, A. et al. (2006) J. Invest. Dermatol. 126:1292.
- Kato, M. et al. (2015) J. Invest. Dermatol. 135:1253.
- Nagasaka, T. et al. (2004) J. Clin. Invest. 114:1484.
- Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.
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