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Recombinant Human DLL4 His-tag Protein

Catalog #: 1506-D4
22 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
1506-D4-050/CF

With Carrier

Catalog # Availability Size / Price Qty
1506-D4-050
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Recombinant Human DLL4 His-tag Protein Bioactivity
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Product Details
Citations (22)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DLL4 His-tag Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 150-600 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human DLL4 protein
Ser27-Pro524, with a C-terminal 10-His tag
Accession #
Q9NR61
N-terminal Sequence
Analysis
Ser27
Predicted Molecular Mass
55.6 kDa
SDS-PAGE
60-70 kDa, reducing conditions

Product Datasheets

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1506-D4 (with carrier)

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1506-D4/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1506-D4

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and PEG 3350 with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1506-D4/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and PEG 3350.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human DLL4 His-tag Protein Bioactivity View Larger

Recombinant Human DLL4 (Catalog # 1506-D4) enhances Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) induced alkaline phosphatase activity in the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 150-600 ng/mL.

SDS-PAGE Recombinant Human DLL4 His-tag Protein SDS-PAGE View Larger

1 µg/lane of Recombinant Human DLL4 was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing major bands at 60-70 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL4

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands result in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

References
  1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
  2. Iso, Tatsuya, et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
  3. Walker, L., et al. (2001) Stem Cells 19:543.
  4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
  5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.
Long Name
Delta-like 4
Entrez Gene IDs
54567 (Human); 54485 (Mouse); 100152163 (Porcine)
Alternate Names
Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4

Citations for Recombinant Human DLL4 His-tag Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Class II phosphatidylinositol 3-kinase-C2&alpha is essential for Notch signaling by regulating the endocytosis of &gamma-secretase in endothelial cells
    Authors: S Shimizu, K Yoshioka, S Aki, Y Takuwa
    Scientific Reports, 2021-03-04;11(1):5199.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2
    Authors: S Kakuda, RK Lopilato, A Ito, RS Haltiwange
    J. Biol. Chem., 2020-08-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Targeting the Notch and TGF-&beta signaling pathways to prevent retinal fibrosis in vitro and in vivo
    Authors: J Fan, W Shen, SR Lee, AE Mathai, R Zhang, G Xu, MC Gillies
    Theranostics, 2020-06-29;10(18):7956-7973.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway
    Authors: SH Chung, W Shen, KC Davidson, A Pébay, RCB Wong, B Yau, M Gillies
    Front Cell Neurosci, 2019-12-03;13(0):527.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Specific NOTCH1 antibody targets DLL4-induced proliferation, migration, and angiogenesis in NOTCH1-mutated CLL cells
    Authors: M López-Guer, S Xargay-Tor, P Fuentes, J Roldán, B González-F, L Rosich, E Silkensted, MJ García-Leó, E Lee-Vergés, N Giménez, A Giró, M Aymerich, N Villamor, J Delgado, A López-Guil, XS Puente, E Campo, ML Toribio, D Colomer
    Oncogene, 2019-10-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Endothelial tip cells in vitro are less glycolytic and have a more flexible response to metabolic stress than non-tip cells
    Authors: B Yetkin-Ari, IMC Vogels, N Neyazi, V van Duinen, RH Houtkooper, CJF van Noorde, I Klaassen, RO Schlingema
    Sci Rep, 2019-07-18;9(1):10414.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. Combined Notch and PDGF Signaling Enhances Migration and Expression of Stem Cell Markers while Inducing Perivascular Cell Features in Muscle Satellite Cells
    Authors: MFM Gerli, LA Moyle, S Benedetti, G Ferrari, E Ucuncu, M Ragazzi, C Constantin, I Louca, H Sakai, P Ala, P De Coppi, S Tajbakhsh, G Cossu, FS Tedesco
    Stem Cell Reports, 2019-02-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy
    Authors: B Kang, J Shin, HJ Park, C Rhyou, D Kang, SJ Lee, YS Yoon, SW Cho, H Lee
    Nat Commun, 2018-12-20;9(1):5402.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Identification and characterization of a novel Sso7d scaffold-based binder against Notch1
    Authors: T Gocha, BM Rao, R DasGupta
    Sci Rep, 2017-09-20;7(1):12021.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability
    Authors: AV Shah, GM Birdsey, C Peghaire, ME Pitulescu, NP Dufton, Y Yang, I Weinberg, L Osuna Alma, L Payne, JC Mason, H Gerhardt, RH Adams, AM Randi
    Nat Commun, 2017-07-11;8(0):16002.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Selective regulation of Notch ligands during angiogenesis is mediated by vimentin
    Authors: D Antfolk, M Sjöqvist, F Cheng, K Isoniemi, CL Duran, A Rivero-Mul, C Antila, R Niemi, S Landor, CVC Bouten, KJ Bayless, JE Eriksson, CM Sahlgren
    Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  12. EphB4 forward signalling mediates angiogenesis caused by CCM3/PDCD10-ablation
    Authors: C You, K Zhao, P Dammann, K Keyvani, I Kreitschma, U Sure, Y Zhu
    J. Cell. Mol. Med., 2017-04-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
    Authors: Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A
    Nat Med, 2015-04-20;21(5):483-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Fatty acid-binding protein 4, a point of convergence for angiogenic and metabolic signaling pathways in endothelial cells.
    Authors: Harjes U, Bridges E, McIntyre A, Fielding B, Harris A
    J Biol Chem, 2014-06-17;289(33):23168-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Dll4-containing exosomes induce capillary sprout retraction in a 3D microenvironment.
    Authors: Sharghi-Namini S, Tan E, Ong L, Ge R, Asada H
    Sci Rep, 2014-02-07;4(0):4031.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Muller glia cells regulate Notch signaling and retinal angiogenesis via the generation of 19,20-dihydroxydocosapentaenoic acid.
    Authors: Hu J, Popp R, Fromel T, Ehling M, Awwad K, Adams R, Hammes H, Fleming I
    J Exp Med, 2014-01-20;211(2):281-95.
  17. Notch signaling plays a critical role in motility and differentiation of human first-trimester cytotrophoblasts.
    Authors: Haider S, Meinhardt G, Velicky P, Otti G, Whitley G, Fiala C, Pollheimer J, Knofler M
    Endocrinology, 2013-12-20;155(1):263-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Loss of CCM3 impairs DLL4-Notch signalling: implication in endothelial angiogenesis and in inherited cerebral cavernous malformations.
    Authors: You C, Sandalcioglu I, Dammann P, Felbor U, Sure U, Zhu Y
    J Cell Mol Med, 2013-02-07;17(3):407-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. The alarmin IL-33 is a notch target in quiescent endothelial cells.
    Authors: Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Kuchler A, Weiss M, Udalova I, Midwood K, Kasprzycka M, Haraldsen G
    Am J Pathol, 2012-07-16;181(3):1099-111.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Ongoing Dll4-Notch signaling is required for T-cell homeostasis in the adult thymus.
    Authors: Billiard F, Kirshner JR, Tait M, Danave A, Taheri S, Zhang W, Waite JC, Olson K, Chen G, Coetzee S, Hylton D, Murphy AJ, Yancopoulos GD, Thurston G, Skokos D
    Eur. J. Immunol., 2011-07-04;41(8):2207-16.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Capture)
  21. Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells.
    Authors: Williams CK, Segarra M, Sierra Mde L, Sainson RC, Tosato G, Harris AL
    Cancer Res., 2008-03-15;68(6):1889-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage.
    Authors: Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L
    Genes Dev., 2008-01-15;22(2):152-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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