Recombinant Human DNAM-1/CD226 His-tag Protein, CF Summary
Product Specifications
Glu19-Asn247, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9298-DN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human DNAM-1/CD226 (Catalog # 9298-ND) is coated at 0.5 µg/mL, 100 µL/well, Recombinant Nectin-2/ CD112 Fc Chimera (Catalog # 9317-N2) binds with an ED50 of 0.4-2 µg/mL.
Reconstitution Calculator
Background: DNAM-1/CD226
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2-6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7-10). Platelet-endothelium interactions mediated by DNAM-1 can enable the metastasis of tumor cells to the lung (11). CD96 competes with DNAM-1 for binding to CD155 and blocks DNAM-1 mediated NK cell activation (12). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 13-15).
- Zingoni, A. et al. (2013) Front. Immunol. 3:408.
- Shibuya, A. et al. (1996) Immunity 4:573.
- Bottino, C. et al. (2003) J. Exp. Med. 198:557.
- Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
- Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
- Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
- Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
- Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
- Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
- Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
- Morimoto, K. et al. (2007) Oncogene 27:264.
- Chan, C.J. et al. (2014) Nat. Immunol. 15:431.
- Shibuya, K. et al. (1999) Immunity 11:615.
- Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
- Shibuya, A. et al. (1998) J. Immunol. 166:1671.
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