Recombinant Human DNER Fc Chimera Protein, CF Summary
Product Specifications
| Human DNER (Arg29 - His637) Accession # NP_620711 | IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3646-DN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: DNER
DNER (Delta/Notch-like EGF-related receptor), also known as BET (brain-specific EGF-like transmembrane protein), is a type I transmembrane glycoprotein of the Notch/Delta family (1, 2). In the mouse, DNER has been detected as 90, 120 and 150 kDa forms which are probably variably glycosylated (1, 2). DNER is specifically expressed on nonaxonal areas of post-mitotic neurons, especially Purkinje cells, but also cortical and hippocampal pyramidal neurons and immature cerebellar granule cells (1 ‑ 5). After expression on the cell surface, DNER is removed from axonal membranes, but remains on somatodendritic membranes (1, 3, 6). A portion of DNER is found within endosomes (1, 3, 6). Human DNER cDNA encodes 737 amino acids (aa) that include a 25 aa signal sequence, a 615 aa extracellular domain (ECD) containing ten distinct Delta/Notch-like EGF‑like repeats, a 21 aa transmembrane sequence, and a 76 aa cytoplasmic domain. The human DNER ECD shares 89%, 89% and 88% aa sequence identity with mouse, rat and bovine DNER, respectively. DNER is a Notch ligand, but is considered a non-classical ligand because it lacks the usual DSL Notch binding motif (5, 7). Instead, Notch interacts with the two EGF‑like repeats closest to the N-terminus of DNER (5). Mice lacking DNER show impaired cerebellar functions and delayed Purkinje cell-mediated maturation of Notch-expressing Bergmann glia during cerebellar development (4, 5). DNER associates with protein tyrosine phosphatase zeta (PTP zeta ), which is the receptor of pleiotrophin (PTN). PTP zeta -PTN-DNER signaling has been implicated in the regulation of neuritogenesis (3). Expression of DNER in glioblastoma stem-like cells inhibits formation of neurospheres in vitro, while in vivo it induces differentiation and inhibits growth of xenografts, thus acting as a tumor suppressor (7). Expression of DNER in adipose‑derived human mesenchymal stem cells and mouse auditory hair cells have also been shown (8, 9).
- Eiraku, M. et al. (2002) J. Biol. Chem. 277:25400.
- Nishizumi, H. et al. (2002) NeuroReport 13:909.
- Fukazawa, N. et al. (2008) Mol. Cell. Biol. 28:4494.
- Tohgo, A. et al. (2005) Mol. Cell. Neurosci. 31:326.
- Eiraku, M. et al. (2005) Nat. Neurosci. 8:873.
- Kurisu, J. et al. (2010) J. Neurochem. 113:1598.
- Sun, P. et al. (2009) Stem Cells 27:1473.
- Park, J-R. et al. (2010) Cell Prolif. 43:19.
- Hartman, B.H. et al. (2010) J. Assoc. Res. Otolaryngol. 11:187.
Citation for Recombinant Human DNER Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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High levels of endothelial ICAM-1 prohibit natalizumab mediated abrogation of CD4+ T cell arrest on the inflamed BBB under flow in vitro
Authors: Soldati, S;B�r, A;Vladymyrov, M;Glavin, D;McGrath, JL;Gosselet, F;Nishihara, H;Goelz, S;Engelhardt, B;
Journal of neuroinflammation
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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