Recombinant Human DSCAM-L1 Protein, CF Summary
Product Specifications
Measured by its ability to bind biotinylated recombinant human DSCAM-L1 in a functional ELISA with an apparent KD <30 nM.
Glu79-Lys1651, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3315-DL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: DSCAM-L1
Down syndrome cell adhesion molecule-like protein 1 (DSCAM-L1; also DSCAM2) is a 224 kDa type I transmembrane glycoprotein and member of the immunoglobulin superfamily (1). Human DSCAM-L1 is a DSCAM paralog located on chromosome 11q23. It is synthesized as a 2053 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1573 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 441 aa cytoplasmic tail. The ECD contains ten Ig-like C2-type domains, six fibronectin type-III domains, and 18 potential sites for N-linked glycosylation. A deletion of aa 34 ‑ 244 produces a second isoform. When compared to DSCAM, DSCAM-L1 shows 64% aa identity to the ECD and 45% aa identity to the cytoplasmic domain (1). Human DSCAM-L1 is 95% aa identical to mouse DSCAM-L1. In the mouse brain, DSCAM-L1 is predominantly expressed in Purkinje cells of the cerebellum, granule cells of the dentate gyrus, and in neurons of the cerebral cortex and olfactory bulb (1). DSCAM-L1 exhibits homophilic binding activity that does not require divalent cations (1). Based on its similarities to DSCAM, it is postulated that DSCAM-L1 is involved in the formation and maintenance of neural networks (1). Because of its chromosomal location, DSCAM-L1 is an ideal candidate for neuronal disorders such as Gilles de la Tourette and Jacobsen syndromes (1). DSCAM-L1 mediates homophilic adhesion and is involved in the formation of lamina-specific synaptic connections in the vertebrate retina (2).
- Agarwala, K.L. et al. (2001) Biochem. Biophys. Res. Commun. 285:760.
- Yamagata M., Sanes JR. (2008) Nature 451(7177):465.
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