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Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF

Catalog #: 724-ES
41 Citations 3 Reviews Datasheet / COA / SDS
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724-ES-100
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Citations (41)
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Reviews (3)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human E-Selectin/Fc Chimera coated plates (2 µg/mL with 100 µL/well), approximately 90-100% will adhere after 1 hour incubation at RT.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human E-Selectin/CD62E protein
Human E-Selectin
(Trp22 - Pro556)
Accession # P16581		 IEGRMD Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus
Accession #
P16581
N-terminal Sequence
Analysis
Trp22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
86 kDa (monomer)
SDS-PAGE
116 - 128 kDa, reducing conditions

Product Datasheets

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724-ES

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

724-ES

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 4 weeks, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E-Selectin/CD62E

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E-Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Entrez Gene IDs
6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)
Alternate Names
CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

Citations for Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. An isoform of the giant protein titin is a master regulator of human T lymphocyte trafficking
    Authors: Toffali, L;D'Ulivo, B;Giagulli, C;Montresor, A;Zenaro, E;Delledonne, M;Rossato, M;Iadarola, B;Sbarbati, A;Bernardi, P;Angelini, G;Rossi, B;Lopez, N;Linke, WA;Unger, A;Di Silvestre, D;Benazzi, L;De Palma, A;Motta, S;Constantin, G;Mauri, P;Laudanna, C;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. T helper 1 effector memory CD4+ T cells protect the skin from poxvirus infection
    Authors: JC Harbour, M Abdelbary, JB Schell, SP Fancher, JJ McLean, TJ Nappi, S Liu, TJ Nice, Z Xia, K Früh, JC Nolz
    Cell Reports, 2023-04-21;42(5):112407.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. A20 and the non-canonical NF-kappaB pathway are key regulators of neutrophil recruitment during fetal ontogeny
    Authors: I Rohwedder, LM Wackerbart, K Heinig, A Ballweg, J Altstätter, M Ripphahn, C Nussbaum, M Salvermose, S Bierschenk, T Straub, M Gunzer, M Schmidt-Su, T Kolben, C Schulz, A Ma, B Walzog, M Heinig, M Sperandio
    JCI Insight, 2023-02-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Sialyltransferase Inhibitor Ac53FaxNeu5Ac Reverts the Malignant Phenotype of Pancreatic Cancer Cells, and Reduces Tumor Volume and Favors T-Cell Infiltrates in Mice
    Authors: L Miró, J López, PE Guerrero, N Martínez-B, N Manero-Rup, M Moreno, MR Ortiz, E Llop, P Navarro, R Peracaula
    Cancers, 2022-12-12;14(24):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  5. Lung-Derived Selectins Enhance Metastatic Behavior of Triple Negative Breast Cancer Cells
    Authors: SU Khan, Y Xia, D Goodale, G Schoettle, AL Allan
    Biomedicines, 2021-10-30;9(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Interferon Gamma Mediates Hematopoietic Stem Cell Activation and Niche Relocalization through BST2
    Authors: MA Florez, KA Matatall, Y Jeong, L Ortinau, PW Shafer, AM Lynch, R Jaksik, M Kimmel, D Park, KY King
    Cell Reports, 2020-12-22;33(12):108530.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. L1CAM as an E-selectin Ligand in Colon Cancer
    Authors: FM Deschepper, R Zoppi, M Pirro, PJ Hensbergen, F Dall'Olio, M Kotsias, RA Gardner, DIR Spencer, PA Videira
    Int J Mol Sci, 2020-11-05;21(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Enhanced sugar chain detection by oriented immobilization of Fc-fused lectins
    Authors: M Iijima, Y Yamada, T Nakayama, S Kuroda
    Biosci. Biotechnol. Biochem., 2020-05-30;0(0):1-5.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  9. Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma
    Authors: U Valentiner, J Knips, R Pries, T Clauditz, A Münscher, G Sauter, B Wollenberg, U Schumacher
    Cancers (Basel), 2019-10-28;11(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay, Flow Cytometry
  10. Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing
    Authors: A Kelkar, Y Zhu, T Groth, G Stolfa, AB Stablewski, N Singhi, M Nemeth, S Neelamegha
    Mol. Ther., 2019-09-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Minimal dosing of leukocyte targeting TRAIL decreases triple-negative breast cancer metastasis following tumor resection
    Authors: N Jyotsana, Z Zhang, LE Himmel, F Yu, MR King
    Sci Adv, 2019-07-24;5(7):eaaw4197.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  12. Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
    Authors: EW Martin, R Malgor, VA Resto, DJ Goetz, MM Burdick
    Sci Rep, 2019-06-11;9(1):8511.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  13. Central memory CD8+ T cells become CD69+ tissue-residents during viral skin infection independent of CD62L-mediated lymph node surveillance
    Authors: JF Osborn, SJ Hobbs, JL Mooster, TN Khan, AM Kilgore, JC Harbour, JC Nolz
    PLoS Pathog., 2019-03-15;15(3):e1007633.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  14. Aberrant O-glycosylation modulates aggressiveness in neuroblastoma
    Authors: HA Cuello, VI Segatori, M Albertó, CA Gulino, R Aschero, S Camarero, LG Mutti, K Madauss, DF Alonso, F Lubienieck, MR Gabri
    Oncotarget, 2018-09-25;9(75):34176-34188.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. P-Selectin Glycoprotein Ligand-1 (PSGL-1) Expressing CD4 T Cells Contribute Plaque Instability in Acute Coronary Syndrome
    Authors: K Kitamura, K Sato, M Sawabe, M Yoshida, N Hagiwara
    Circ. J., 2018-06-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates
    Authors: NM Cordina, N Sayyadi, LM Parker, A Everest-Da, LJ Brown, NH Packer
    Sci Rep, 2018-03-14;8(1):4521.
    Applications: Bioassay
  17. C/EBP? drives interactions between human MAIT cells and endothelial cells that are important for extravasation
    Authors: CH Lee, HH Zhang, SP Singh, L Koo, J Kabat, H Tsang, TP Singh, JM Farber
    Elife, 2018-02-22;7(0):.
    Species: Human
    Sample Types:
    Applications: Bioassay
  18. Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium
    Authors: FW Lam, Q Da, B Guillory, MA Cruz
    J. Immunol., 2018-01-15;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  19. A Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells
    Stem Cells, 2017-02-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. SOS1, ARHGEF1, and DOCK2 rho-GEFs Mediate JAK-Dependent LFA-1 Activation by Chemokines
    Authors: Lara Toffali
    J. Immunol, 2016-12-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. E-selectin Targeting PEGylated-thioaptamer Prevents Breast Cancer Metastases
    Authors: Yoshihiro Morita
    Mol Ther Nucleic Acids, 2016-12-13;5(12):e399.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  22. Development of Polymer Microcapsules Functionalized with Fucoidan to Target P-Selectin Overexpressed in Cardiovascular Diseases
    Authors: Bo Li
    Adv Healthc Mater, 2016-12-12;0(0):.
    Applications: Bioassay
  23. Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study
    Authors: Benjamin Malard
    Artif Organs, 2016-12-02;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Hemofiltration Procedure
  24. Non-fucosylated CB CD34(+) cells represent a good target for enforced fucosylation to improve engraftment following cord blood transplantation
    Authors: Simon N Robinson
    Cytotherapy, 2016-12-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion
    Sci Rep, 2016-07-26;6(0):30392.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo.
    Authors: Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E
    Nat Commun, 2015-03-31;6(0):6387.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Blocking the adhesion cascade at the premetastatic niche for prevention of breast cancer metastasis.
    Authors: Kang S, Hasan N, Mann A, Zheng W, Zhao L, Morris L, Zhu W, Zhao Y, Suh K, Dooley W, Volk D, Gorenstein D, Cristofanilli M, Rui H, Tanaka T
    Mol Ther, 2015-03-27;23(6):1044-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  28. Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes.
    Authors: Ayala-Lujan, Jorge Lu, Vijayakumar, Vidhya, Gong, Mei, Smith, Rachel, Santiago, Araceli, Ruiz-Perez, Fernando
    PLoS ONE, 2014-09-24;9(9):e107920.
    Species: Bacteria, Human
    Sample Types: Protein, Whole Cells
    Applications: Enzyme Assay, Enzyme Assay Substrate
  29. Selectins mediate small cell lung cancer systemic metastasis.
    Authors: Heidemann F, Schildt A, Schmid K, Bruns O, Riecken K, Jung C, Ittrich H, Wicklein D, Reimer R, Fehse B, Heeren J, Luers G, Schumacher U, Heine M
    PLoS ONE, 2014-04-03;9(4):e92327.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. SDA, a DNA aptamer inhibiting E- and P-selectin mediated adhesion of cancer and leukemia cells, the first and pivotal step in transendothelial migration during metastasis formation.
    Authors: Faryammanesh R, Lange T, Magbanua E, Haas S, Meyer C, Wicklein D, Schumacher U, Hahn U
    PLoS ONE, 2014-04-03;9(4):e93173.
    Species: Human
    Sample Types: Beads
    Applications: Bioassay
  31. IL-15 regulates memory CD8+ T cell O-glycan synthesis and affects trafficking.
    Authors: Nolz J, Harty J
    J Clin Invest, 2014-02-10;124(3):1013-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Heterotropic modulation of selectin affinity by allosteric antibodies affects leukocyte rolling.
    Authors: Riese S, Kuehne C, Tedder T, Hallmann R, Hohenester E, Buscher K
    J Immunol, 2014-01-15;192(4):1862-9.
    Species: Human
    Sample Types: Protein, Whole Cells
    Applications: Bioassay, Flow Cytometry
  33. Circulating tumor cells from prostate cancer patients interact with E-selectin under physiologic blood flow.
    Authors: Gakhar, Gunjan, Navarro, Vicente, Jurish, Madelyn, Lee, Guang Yu, Tagawa, Scott T, Akhtar, Naveed H, Seandel, Marco, Geng, Yue, Liu, He, Bander, Neil H, Giannakakou, Paraskev, Christos, Paul J, King, Michael, Nanus, David M
    PLoS ONE, 2013-12-27;8(12):e85143.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice.
    Authors: Domingos-Pereira S, Decrausaz L, Derre L, Bobst M, Romero P, Schiller J, Jichlinski P, Nardelli-Haefliger D
    Mucosal Immunol, 2012-09-12;6(2):393-404.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Survival advantages conferred to colon cancer cells by E-selectin-induced activation of the PI3K-NFkappaB survival axis downstream of Death receptor-3.
    Authors: Porquet N, Poirier A, Houle F, Pin AL, Gout S, Tremblay PL, Paquet ER, Klinck R, Auger FA, Huot J
    BMC Cancer, 2011-07-01;11(0):285.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    Authors: Kato N, Yuzawa Y, Kosugi T
    J. Am. Soc. Nephrol., 2009-05-14;20(7):1565-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Binding Assay
  37. CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1.
    Authors: Nonomura C, Kikuchi J, Kiyokawa N, Ozaki H, Mitsunaga K, Ando H, Kanamori A, Kannagi R, Fujimoto J, Muroi K, Furukawa Y, Nakamura M
    Cancer Res., 2008-02-01;68(3):790-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  38. Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)(+) CD4(+) T cells.
    Authors: Ni</LastName><ForeName>Zh Z</Initial, Ni Z, Walcheck B
    Immunol. Lett., 2007-01-03;108(2):179-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. The monoclonal antibody CHO-131 identifies a subset of cutaneous lymphocyte-associated antigen T cells enriched in P-selectin-binding cells.
    Authors: Ni Z, Campbell JJ, Niehans G, Walcheck B
    J. Immunol., 2006-10-01;177(7):4742-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  40. The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors.
    Authors: Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS
    J. Immunol., 2006-10-01;177(7):4488-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. In vitro differentiation from naive to mature E-selectin binding CD4 T cells: acquisition of skin-homing properties occurs independently of cutaneous lymphocyte antigen expression.
    Authors: Takahashi R, Mizukawa Y, Yamazaki Y, Hayakawa K, Hayakawa J, Kudo A, Shiohara T
    J. Immunol., 2003-12-01;171(11):5769-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF

Average Rating: 4.3 (Based on 3 Reviews)

5 Star
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Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF
By JIWON LEE on 03/06/2020
Application: Immunoassay Standard
Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF
By Anonymous on 10/27/2018
Application: Binding assay/Protein-protein interaction
Reason for Rating: This product does not produce as strong a signal as 575-ES (mouse E-selectin). I am not sure if this relates to preparation or binding kinetics, however the mouse E-selectin is more broadly used in the literature, even when binding to human cell lines.

Used both mouse and human at 10 ug/mL for the flow cytometry binding assay, with Biolegend anti-human IgG-PE used as a secondary probe.

Binding assay/Protein-protein interaction Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF 724-ES
Recombinant Human E-Selectin/CD62E Fc Chimera Protein, CF
By Anonymous on 12/15/2016
Application: Binding assay/Protein-protein interaction

We treat differentiated iPSC and use this product to measure the ability of the treated cells to bind to E-Selectin.