Recombinant Human EDA-A1/Ectodysplasin A1 Protein
Recombinant Human EDA-A1/Ectodysplasin A1 Protein Summary
Product Specifications
Ser160-Ser391 & Lys178-Ser391
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3944-ED
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
3944-ED/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: EDA-A1/Ectodysplasin A1
Ectodysplasin is a 45 kDa type II transmembrane TNF superfamily protein that is associated with X-linked hypohidrotic ectodermal dysplasia (HED), a disorder of hair, tooth, and sweat gland development (1 - 4). The human EDA-A1 cDNA encodes a 41 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment, and a 329 aa extracellular region that contains a terminal TNF homology domain, a collagenous domain, and a stalk region (3, 5, 6). Within the collagenous and TNF homology domains, human EDA-A1 shares greater than 97% aa sequence identity with bovine, canine, mouse, and rat EDA-A1. Multiple alternately spliced EDA variants have been described (4, 7). The dominant variant, EDA-A2, has a deletion of two amino acids that changes the receptor binding selectivity from EDAR to XEDAR (4, 7, 8). The collagenous domain of EDA-A1 mediates non-covalent homotrimer formation (5, 6). Shedding of the collagenous and TNF homology domains of EDA-A1 is accomplished by a furin-like protease. The released fragment maintains its trimeric state and ability to bind EDAR (9, 10). Some EDA-A1 polymorphisms found in HED patients alter the protease recognition site and prevent shedding (9). EDA-A1 is expressed in developing hair follicles, epidermis, teeth, sweat glands, salivary glands, and forebrain (6, 8, 11 - 13). It regulates ectodermal appendage formation and is critical to the patterning and morphogenesis of hair follicles, partially through the induction of Lymphotoxin beta (5, 12, 14). Receptor and ligand expression are regulated by factors involved in many aspects of tissue morphogenesis. EDA-A1 expression is induced by Wnt6 (12, 13), while the expression of EDAR is induced by Activin beta A and inhibited by BMP-2, -4, and -7 (13, 15).
- Mikkola, M.L. and I. Thesleff (2003) Cytokine Growth Factor Rev. 14:211.
- Botchkarev, V.A. and M.Y. Fessing (2005) J. Invest. Dermatol. 10:247.
- Kere, J. et al. (1996) Nat. Genet. 13:409.
- Bayes, M. et al. (1998) Hum. Mol. Genet. 7:1661.
- Ezer, S. et al. (1999) Hum. Mol. Genet. 8:2079.
- Mikkola, M.L. et al. (1999) Mech. Dev. 88:133.
- Hashimoto, T. et al. (2006) Gene 371:42.
- Yan, M. et al. (2000) Science 290:523.
- Chen, Y. et al. (2001) Proc. Natl. Acad. Sci. 98:7218.
- Elomaa, O. et al. (2001) Hum. Mol. Genet. 10:953.
- Pispa, J. et al. (2003) Gene Exp. Patterns 3:675.
- Laurikkala, J. et al. (2002) Development 129:2541.
- Laurikkala, J. et al. (2001) Dev. Biol. 229:443.
- Cui, C.Y. et al. (2006) Proc. Natl. Acad. Sci. 103:9142.
- Mou, C. et al. (2006) Proc. Natl. Acad. Sci. 103:9075.
Citations for Recombinant Human EDA-A1/Ectodysplasin A1 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Induction of periodontal ligament-derived mesenchymal stromal cell-like cells from human induced pluripotent stem cells
Authors: Wang, J;Morita, K;Iwata, T;
Regenerative therapy
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
EDA2R-NIK signalling promotes muscle atrophy linked to cancer cachexia
Authors: Bilgic, SN;Domaniku, A;Toledo, B;Agca, S;Weber, BZC;Arabaci, DH;Ozornek, Z;Lause, P;Thissen, JP;Loumaye, A;Kir, S;
Nature
Species: Mouse
Sample Types: Transduced Whole Cells, Whole Cells
Applications: Bioassay -
An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
MAbs, 2018-03-05;0(0):1-43.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
Ectodysplasin A protein promotes corneal epithelial cell proliferation
Authors: S Li, J Zhou, J Bu, K Ning, L Zhang, J Li, Y Guo, X He, H He, X Cai, Y Chen, PS Reinach, Z Liu, W Li
J. Biol. Chem., 2017-06-27;292(32):13391-13401.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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