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Recombinant Human ELFN2 Fc Chimera Protein, CF

Catalog #: 10643-EF Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10643-EF-050
Recombinant Human ELFN2 Fc Chimera Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ELFN2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human mGluR7 Protein (Catalog # 9530-UR) is coated at 1 μg/mL, 100 μL/well, Recombinant Human ELFN2 Fc Chimera binds with an ED50 of 0.15-0.75 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human ELFN2 protein
Human ELFN2
(Asp23-Tyr397)
Accession # Q5R3F8.1		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
Q5R3F8.1
N-terminal Sequence
Analysis
Asp23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
69 kDa
SDS-PAGE
97-112 kDa, under reducing conditions

Product Datasheets

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10643-EF

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10643-EF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human mGluR7 Protein (9530-UR) is coated at 1 μg/mL, 100 μL/well, Recombinant Human ELFN2 Fc Chimera binds with an ED50 of 0.15-0.75 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human ELFN2 Fc Chimera Protein (Catalog # 10643-EF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 97-112 kDa and 194-224 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ELFN2

Extracellular leucine-rich repeat and fibronectin type III domain-containing protein 2 (ELFN2), also known as protein phosphatase 1 regulatory subunit 29 (PPP1R29), is a member of the extracellular leucine-rich repeat (LRR) superfamily (1). Human ELFN2 consists of an extracellular domain (ECD) containing five LRR domains, one LRR C-terminal (LRRCT) domain and an FN3 domain, a transmembrane domain, and a cytoplasmic domain. Within the mature ECD, human ELFN2 shares 98% amino acid sequence identity with mouse and rat ELFN2. ELFN-2 is quite restricted to the nervous system in which it is broadly expressed in the cortex,  presumed glutamatergic neurons, hippocampus, pyramidal and granule cells, and in the striatum. It has been proposed that ELFN-2 could provide the requisite specificity of cellular interactions to mediate a large number of selective connectivity decisions thereby involved in neurite outgrowth, axon guidance, fasciculation, and synapse formation (2). ELFN2 has been identified as a novel postsynaptic adhesion molecule expressed throughout the brain (3). Mutations of ELFN2 have been reported in pancreatic cancer radiation resistance (4) and stress fracture (5).



References
  1. Liu, C. et al. (2018) Mol. Ther. 26:2267.
  2. Dolan, J. et al. (2007) BMC Genomics 8:320.
  3. Dunn, H.A. et al. (2019) Mol. Psychiatry 24:1902.
  4. Friedman E. et al. (2014) Genet. Res. (Camb) 96:e004.
  5. Souchek L.L. et al. (2014) Br. J. Cancer 111:1139.
Long Name
Extracellular Leucine-Rich Repeat And Fibronectin Type III Domain Containing 2
Entrez Gene IDs
114794 (Human); 207393 (Mouse)
Alternate Names
dJ63G5.3 (putative Leucine rich protein); dJ63G5.3; ELFN2; extracellular leucine-rich repeat and fibronectin type III containing 2; extracellular leucine-rich repeat and fibronectin type III domain containing 2; extracellular leucine-rich repeat and fibronectin type III domain-containing protein 2; KIAA1904; leucine rich repeat containing 62; leucine-rich repeat and fibronectin type-III domain-containing protein 6; leucine-rich repeat-containing protein 62; LRRC62; PPP1R29; protein phosphatase 1, regulatory subunit 29

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